Recombinant Mouse Breast cancer anti-estrogen resistance protein 1 (Bcar1), partial

中文名稱：

Recombinant Mouse Breast cancer anti-estrogen resistance protein 1(Bcar1) ,partial
貨號：

CSB-YP733774MO
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Mouse Breast cancer anti-estrogen resistance protein 1(Bcar1) ,partial
貨號：

CSB-EP733774MO
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Mouse Breast cancer anti-estrogen resistance protein 1(Bcar1) ,partial
貨號：

CSB-EP733774MO-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Mouse Breast cancer anti-estrogen resistance protein 1(Bcar1) ,partial
貨號：

CSB-BP733774MO
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Mouse Breast cancer anti-estrogen resistance protein 1(Bcar1) ,partial
貨號：

CSB-MP733774MO
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Bcar1
Uniprot No.：

Q61140
別名：

Bcar1; Cas; CrkasBreast cancer anti-estrogen resistance protein 1; CRK-associated substrate; p130cas
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration and cell branching. Involved in the BCAR3-mediated inhibition of TGFB signaling.
基因功能參考文獻：
1. CAS localizes to the force transduction layer of focal adhesions and mechanical stress stabilizes CAS in focal adhesions. PMID: 28406229
2. Data suggest that the first LD motif (LD1; leucine-rich motif) of paxillin binds to the CCHD (C-terminal region of Cas family homology domain) of p130Cas/Bcar1 (in a 1:1 stoichiometry with Kd approximately 4.2 uM); p130Cas/Bcar1 CCHD is stabilized by forming complex with paxillin LD1; these studies utilized chicken paxillin and mouse p130Cas/Bcar1. (p130Cas/Bcar1 = breast cancer anti-estrogen resistance protein 1) PMID: 28860193
3. reduction of p130Cas levels by siRNA affects process outgrowth, the thickness of cellular processes and migration behavior of Oli-neu cells. long term p130Cas reduction results in increased apoptosis in cultured primary oligodendrocytes. PMID: 24586768
4. CRP2 sequesters p130Cas at focal adhesions, thereby reducing lamellipodia formation and blunting vascular smooth muscle cell migration. PMID: 23975851
5. p130Cas plays pivotal roles in osteoclastic bone resorption. PMID: 23526406
6. 31-kDa caspase-generated cleavage product of p130Cas inhibited MyoD-induced transcriptional activation of muscle-specific genes. PMID: 24472550
7. beta1-Integrin signaling within migrating ganglion cell layer cells requires Cas signaling-adaptor proteins PMID: 24559672
8. Crk-associated substrate -vinculin binding significantly affects the dynamics of Crk-associated substrate and vinculin within focal adhesions as well as the size of focal adhesions. PMID: 23974298
9. we provide evidence that exercise-induced p38 MAPK signaling is not impaired by the muscle-specific deletion of p130Cas. We conclude that p130Cas plays a limited role in mechanical-stress-induced skeletal muscle adaptation. PMID: 23325412
10. study described a molecular complex of PTPalpha-BCAR3-Cas-Src; this complex forms in response to PTPalpha Tyr789 phosphorylation and mediates Cas localization to focal adhesions and Cas downstream signaling to promote cell migration PMID: 22801373
11. p130Cas phosphorylation, mediated by integrin beta3, facilitates cofilin inactivation and promotes myogenic differentiation through modulating actin cytoskeleton remodelling PMID: 22587391
12. Cas is required for apoptosis that is induced by proteasome inhibition, and potentially by other death stimuli. PMID: 22034875
13. results demonstrate that p130Cas is an essential transducer in ErbB2 transformation and highlight its potential use as a novel therapeutic target in ErbB2 positive human breast cancers PMID: 20505116
14. Cas plays pivotal roles in liver development through the reorganization of the actin cytoskeleton and formation of fenestrae in sinusoidal endothelial cells. PMID: 20623582
15. The Cas utilizes Nck2 to activate Cdc42 and induce cell polarization in response to wounding. PMID: 20637038
16. Results describe the role of nitric oxide in regulating the focal adhesion proteins, Src, FAK, p130 Cas, and PTP-alpha. PMID: 20055753
17. regulation of tyrosine phosphorylation by focal adhesion kinase PMID: 11787067
18. Fluoroaluminate stimulates phosphorylation of p130 Cas and Fak and increases attachment and spreading of preosteoblastic MC3T3-E1 cells. PMID: 11792571
19. hyperphosphorylation in STAT3 deficiency in keratinocytes leads to compromised cell migration PMID: 11812786
20. Mouse embryonic fibroblasts (MEFs) derived from FAK(-/-) and p130(cas-/-) mice had severe defects in ephrinA1-induced cell spreading. PMID: 12134157
21. Cas SH3 domain selectively inhibited v-Src-stimulated activations of AKT and JNK, but not ERK and STAT3. PMID: 14741371
22. CAS is a target for phosphorylation both by FAK-dependent and -independent pathways after integrin ligation PMID: 15034138
23. endogenous Sin influences T-lymphocyte signaling by sequestering signaling substrates and regulating their availability PMID: 15121874
24. ELevated CAS-associated tyrosine phosphorylation signaling events occurring at sites of integrin-mediated cell adhesion can have a major role in the development of an invasive cell phenotype. PMID: 15273716
25. FAK may increase the susceptibility of v-Src-transformed cells to anoikis by modulating the level of p130(Cas) PMID: 16132110
26. Cas acts as a key scaffold that links the proteins associated with tyrosine phosphorylation signaling pathways to the granule cell axon elongation. PMID: 16687575
27. Cas phosphorylation sites are dispensable for processive phosphorylation by Src and anchorage-independent cell growth PMID: 16707485
28. S. typhimurium induces formation of focal adhesion-like complexes at sites of bacterial attachment and that both focal adhesion kinase (FAK) and the scaffolding protein p130Cas are required for Salmonella uptake. PMID: 16914515
29. Src utilizes Cas to inhibit gap junctional communication mediated by Cx43 PMID: 17488714
30. These findings suggest that focal adhesion targeting of v-Crk is essential in v-Crk-mediated cellular signaling and that v-Crk must form a complex with p130CAS or a p130CAS substitute to transduce signaling from the extracellular matrix. PMID: 17786957
31. analyzed downstream signaling pathways regulated by Cas exon 2 PMID: 18233957
32. These findings indicate that v-Crk controls cell migration and membrane dynamics by activating Rac in CAS-deficient mouse embryo fibroblasts. PMID: 18319625
33. p130Cas-mediated control of TGF-beta/Smad signaling may provide an additional clue to the mechanism underlying resistance to TGF-beta-induced growth inhibition. PMID: 18321991
34. The FAK-p130Cas signalling axis only provides an anti-apoptotic signal in fibroblasts PMID: 19126677
35. Abl differentially regulates Crk-associated substrate, vinculin, and paxillin in arterial vessels PMID: 19542491
36. p130Cas is required for mammary tumor growth and transforming growth factor-beta-mediated metastasis through regulation of Smad2/3 activity PMID: 19822523
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亞細胞定位：

Cell junction, focal adhesion. Cytoplasm. Cell projection, axon.
蛋白家族：

CAS family
組織特異性：

Expressed in olfactory sensory neurons (at protein level). Expressed abundantly in the liver, lung, brain, and at lower levels in the heart (at protein level).
數據庫鏈接：

KEGG: mmu:12927

STRING: 10090.ENSMUSP00000129584

UniGene: Mm.3758