Recombinant Mouse B-lymphocyte antigen CD20 (Ms4a1), partial

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中文名稱：

小鼠Ms4a1重組蛋白
貨號：

CSB-YP015007MO1
說明書：

MSDS Datasheet
規格：

￥2208
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

The purity of Ms4a1 was greater than 95% as determined by SEC-HPLC
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產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.
基因名：

Ms4a1
Uniprot No.：

P19437
別名：

Ms4a1; Cd20; Ly-44; Ms4a2; B-lymphocyte antigen CD20; B-cell differentiation antigen Ly-44; Lymphocyte antigen 44; Membrane-spanning 4-domains subfamily A member 1; CD antigen CD20
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
來源：

Yeast
分子量：

22.3kDa
表達區域：

111-291aa
氨基酸序列

VIMSSLSLFAAISGIILSIMDILNMTLSHFLKMRRLELIQTSKPYVDIYDCEPSNSSEKNSPSTQYCNSIQSVFLGILSAMLISAFFQKLVTAGIVENEWKRMCTRSKSNVVLLSAGEKNEQTIKMKEEIIELSGVSSQPKNEEEIEIIPVQEEEEEEAEINFPAPPQEQESLPVENEIAP
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

CUSABIO synthesized the recombinant gene by integrating the N-terminal 6xHis tag sequence into the targeted gene encoding the 111-291aa of the mouse Ms4a1. The synthesized gene was subsequently cloned into an expression vector. After cloning, the expression vector was introduced into the yeast for expression. The product was purified to obtain the recombinant mouse Ms4a1 protein carrying N-terminal 6xHis tag. The SDS-PAGE assayed the purity of this recombinant Ms4a1 protein greater than 90%. This Ms4a1 protein migrated along the gel to a band of about 21 kDa molecular weight.

B-lymphocyte antigen CD20 is a protein encoding by a gene named Ms4a1 in mouse and a gene named MS4A1 in human. CD20 is a specific marker on the surface of B lymphocytes. It is expressed on the surface of more than 95% of normal or malignant B lymphocytes. There is no expression of CD20 in hematopoietic stem cells, progenitor cells and other normal tissues. Studies have shown that CD20 has the function of calcium ion channels, which can affect the cell cycle, regulate cell proliferation and differentiation, and even lead to the occurrence of cell apoptosis by regulating the concentration of calcium ions in cells.
Datasheet & COA：

Please contact us to get it.

產品評價

問答及客戶評論

■ 常見問題解答

I am asking about the purpose of the SUMO tag. Is it just for solubilzation? Can you please send the sequence with the tag?

Thanks for your inquiry. CSB-YP015007MO1 is N-terminal 6xHis-tagged, not N-terminal 6xHis-SUMO-tagged. Kindly confirm.The complete sequence is as follows.

EAEAYVHHHHHHEFRT + VIMSSLSLFAAISGIILSIMDILNMTLSHFLKMRRLELIQTSKPYVDIYDCEPSNSSEKNSPSTQYCNSIQSVFLGILSAMLISAFFQKLVTAGIVENEWKRMCTRSKSNVVLLSAGEKNEQTIKMKEEIIELSGVSSQPKNEEEIEIIPVQEEEEEEAEINFPAPPQEQESLPVENEIAP

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靶點詳情

功能：

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR.
基因功能參考文獻：
1. Our findings indicate that anti-CD20-IFNalpha eradicates B-cell lymphoma by employing tumor cells as antigen-presenting cells to reactivate tumor-infiltrating CD8(+) T cells and synergizing with anti-PD-L1 treatment PMID: 28533311
2. Galectin-1 has a role in driving lymphoma CD20 immunotherapy resistance in a mouse model PMID: 26888257
3. Results indicate that an antigen-specific B cell response towards intracellular pathogens can be generated during anti-CD20 depletion therapy. PMID: 26261496
4. this study demonstrates a role for CD20 in B cell activation and T-dependent humoral immunity. PMID: 23966626
5. CD20 is required for store-operated calcium entry in C2C12 myoblasts. PMID: 22982241
6. immunization of mice with the CD20 extracellular domain-6 using Freund's or QS-21 adjuvants was shown to exert significant biological effects in vivo with the pronounced depletion of splenic B cells. PMID: 20189250
7. type I CD20 antibody cytotoxicity critically depends on Fc receptor ITAM signaling PMID: 20354182
8. CD20 deficiency in humans results in impaired T cell-independent antibody responses. PMID: 20038800
9. review of structure, function, tissue and cell distribution PMID: 12144126
10. Anti-CD20 antibodies rapidly depleted the vast majority of circulating and tissue B cells in an isotype-restricted manner dependent on effector cell Fc receptor expression. PMID: 15210744
11. expression in bacterial cells; isolation and biophysical and structural studies PMID: 16285718
12. In mice treated with anti-CD20 mAb, development of arthritis was significantly reduced in comparison to control mAb-treated mice PMID: 18354225
13. involvement of CD20 in calcium influx PMID: 18474602
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亞細胞定位：

Cell membrane; Multi-pass membrane protein. Cell membrane; Lipid-anchor.
蛋白家族：

MS4A family
數據庫鏈接：

KEGG: mmu:12482

STRING: 10090.ENSMUSP00000126422

UniGene: Mm.4046