Recombinant Listeria monocytogenes serovar 1/2a Listeriolysin regulatory protein (prfA)

中文名稱：

單核增生李斯特菌血清1/2aprfA重組蛋白
貨號：

CSB-YP325257LPY
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

單核增生李斯特菌血清1/2aprfA重組蛋白
貨號：

CSB-EP325257LPY
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

單核增生李斯特菌血清1/2aprfA重組蛋白
貨號：

CSB-EP325257LPY-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

單核增生李斯特菌血清1/2aprfA重組蛋白
貨號：

CSB-BP325257LPY
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

單核增生李斯特菌血清1/2aprfA重組蛋白
貨號：

CSB-MP325257LPY
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

prfA
Uniprot No.：

P22262
別名：

prfA; lmo0200; Listeriolysin regulatory protein
種屬：

Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
蛋白長度：

full length protein
表達區(qū)域：

1-237
氨基酸序列

MNAQAEEFKK YLETNGIKPK QFHKKELIFN QWDPQEYCIF LYDGITKLTS ISENGTIMNL QYYKGAFVIM SGFIDTETSV GYYNLEVISE QATAYVIKIN ELKELLSKNL THFFYVFQTL QKQVSYSLAK FNDFSINGKL GSICGQLLIL TYVYGKETPD GIKITLDNLT MQELGYSSGI AHSSAVSRII SKLKQEKVIV YKNSCFYVQN LDYLKRYAPK LDEWFYLACP ATWGKLN
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Positively regulates expression of listeriolysin, of 1-phosphadidylinositol phosphodiesterase (PI-PLC) and other virulence factors.
基因功能參考文獻：
1. Spontaneous loss of virulence in natural populations of Listeria monocytogenes due to mutations in hlyA and PrfA has been demonstrated. PMID: 28827366
2. PepP peptidase plays a role in posttranslational activation of the major virulence regulator, PrfA. PepP mediates recruitment of PrfA to the cytoplasmic membrane. PMID: 28396325
3. The truncated C-terminus of prfA considerably contributes to the strongly attenuated phenotype observed in vitro. PMID: 25813546
4. A novel bile exclusion locus bilE, coordinately regulated by sigmaB and PrfA, represents a new and important virulence factor in L. monocytogenes. PMID: 15686563
5. PrfA K220T amino acid substituted mutant gene in an EGDDeltaprfA strain did not restore the haemolytic and phosphatidylcholine phospholipase C activities, in contrast to the wild-type prfA gene. PMID: 17379709
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數(shù)據(jù)庫鏈接：

KEGG: lmo:lmo0200

STRING: 169963.lmo0200