Recombinant Influenza A virus Polymerase basic protein 2 (PB2), partial

中文名稱：

Recombinant Influenza A virus Polymerase basic protein 2(PB2) ,partial
貨號：

CSB-YP868724IEF
規(guī)格：
來源：

Yeast
其他：

中文名稱：

Recombinant Influenza A virus Polymerase basic protein 2(PB2) ,partial
貨號：

CSB-EP868724IEF
規(guī)格：
來源：

E.coli
其他：

中文名稱：

Recombinant Influenza A virus Polymerase basic protein 2(PB2) ,partial
貨號：

CSB-EP868724IEF-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Influenza A virus Polymerase basic protein 2(PB2) ,partial
貨號：

CSB-BP868724IEF
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Influenza A virus Polymerase basic protein 2(PB2) ,partial
貨號：

CSB-MP868724IEF
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

PB2
Uniprot No.：

Q9Q0V1
別名：

PB2Polymerase basic protein 2; RNA-directed RNA polymerase subunit P3
種屬：

Influenza A virus (strain A/Goose/Guangdong/1/1996 H5N1 genotype Gs/Gd)
蛋白長度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Plays an essential role in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. Recognizes and binds the 7-methylguanosine-containing cap of the target pre-RNA which is subsequently cleaved after 10-13 nucleotides by the viral protein PA. Plays a role in the initiation of the viral genome replication and modulates the activity of the ribonucleoprotein (RNP) complex.
基因功能參考文獻(xiàn)：
1. Substitution of the influenza-virus polymerase basic protein 2 (PB2) gene segment of the H5N8 virus by that from the H5N1 virus results in a increased virulence for mice compared with the parental virus. The reassortant virus also induced the highest virus titers in lungs and the highest levels of inflammatory cytokine responses. PMID: 28873012
2. Two miRNAs, miR-584-5p and miR-1249, that matched with the PB2 binding sequence, dramatically down-regulated PB2 expression, and inhibited replication of H5N1 and H1N1 influenza A virus strains in A549 cells. PMID: 28609011
3. Using NMR chemical shift perturbation assays we show that amino acid region 601 to 607 of the PB2 '627' domain interacts with a fragment of NP. Substitutions of these PB2 amino acids resulted in diminished RNP activity and surface plasmon resonance assays showed that amino acids D605 was essential for the interaction with NP and V606 may also play a partial role in the interaction. PMID: 29338047
4. PB2-K526R interacts with nuclear export protein and our results suggest that it contributes to enhance replication for certain influenza virus subtypes, particularly in combination with 627K. PMID: 25409547
5. Novel residues in avian influenza virus PB2 protein affect virulence in mammalian hosts. PMID: 25289523
6. The results show that mutations in PB2 arise during adaptation of an avian influenza A virus to human respiratory cells and that the mutations confer efficient growth. PMID: 25210184
7. PB2 mutation D701N plays an important role in virus adaptation. PMID: 24899203
8. Mutation D701N of PB2 promotes importin-alpha mediated nuclear transport in mammalian cells. [review] PMID: 23732876
9. The PB2 (I63T) and PB1 (T677M) mutations decrease the virulence in mice. PMID: 21367983
10. influenza polymerase PB2 is one of the determinants of pathogenicity of duck H5N1 viruses. PMID: 20211480
11. Exchange of the PB1, PB2, and NP genes impacted replication of the reassortant viruses while also affecting the expression of specific host genes PMID: 18305037
12. PB2 interaction with alpha-importins is required for virus RNA replication. PMID: 19066626
13. PB2 amino acids 627 and 701 are determinants of mammalian inter-host transmission in diverse virus backgrounds PMID: 19119420
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亞細(xì)胞定位：

Virion. Host nucleus.
蛋白家族：

Influenza viruses PB2 family
數(shù)據(jù)庫鏈接：

KEGG: vg:3654615