Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Nef (nef)

中文名稱：

Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Nef(nef)
貨號：

CSB-YP324101HKZ
規(guī)格：
來源：

Yeast
其他：

中文名稱：

Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Nef(nef)
貨號：

CSB-EP324101HKZ
規(guī)格：
來源：

E.coli
其他：

中文名稱：

Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Nef(nef)
貨號：

CSB-EP324101HKZ-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Nef(nef)
貨號：

CSB-BP324101HKZ
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Nef(nef)
貨號：

CSB-MP324101HKZ
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

nef
Uniprot No.：

P20886
別名：

nef; Protein Nef; 3'ORF; Negative factor; F-protein) [Cleaved into: C-terminal core protein]
種屬：

Human immunodeficiency virus type 1 group M subtype B (isolate OYI) (HIV-1)
蛋白長度：

Full Length of Mature Protein
表達區(qū)域：

2-211
氨基酸序列

GGKWSKCSM KGWPTIRERM KRAELQPPEP AAEGVGAASR DLEKHGAITS SNTAATNADC AWLEAQEDEE VGFPVRPQVP LRPMTYKGAL DLSHFLKEKG GLEGLIYSQK RQDILDLWVY HTQGYFPDWQ NYTPGPGIRY PLCFGWCFKL VPMDPDQVEE ANEGENNSLL HPISLHGMDD PEKEVLVWKF DSRLAFRHMA REVHPEYYKD C
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Factor of infectivity and pathogenicity, required for optimal virus replication. Alters numerous pathways of T-lymphocytes function and down-regulates immunity surface molecules in order to evade host defense and increase viral infectivity. Alters the functionality of other immunity cells, like dendritic cells, monocytes/macrophages and NK cells.; In infected CD4(+) T-lymphocytes, down-regulates the surface MHC-I, mature MHC-II, CD4, CD28, CCR5 and CXCR4 molecules. Mediates internalization and degradation of host CD4 through the interaction of with the cytoplasmic tail of CD4, the recruitment of AP-2 (clathrin adapter protein complex 2), internalization through clathrin coated pits, and subsequent transport to endosomes and lysosomes for degradation. Diverts host MHC-I molecules to the trans-Golgi network-associated endosomal compartments by an endocytic pathway to finally target them for degradation. MHC-I down-regulation may involve AP-1 (clathrin adapter protein complex 1) or possibly Src family kinase-ZAP70/Syk-PI3K cascade recruited by PACS2. In consequence infected cells are masked for immune recognition by cytotoxic T-lymphocytes. Decreasing the number of immune receptors also prevents reinfection by more HIV particles (superinfection). Down-regulates host SERINC3 and SERINC5 thereby excluding these proteins from the viral particles. Virion infectivity is drastically higher when SERINC3 or SERINC5 are excluded from the viral envelope, because these host antiviral proteins impare the membrane fusion event necessary for subsequent virion penetration.; Bypasses host T-cell signaling by inducing a transcriptional program nearly identical to that of anti-CD3 cell activation. Interaction with TCR-zeta chain up-regulates the Fas ligand (FasL). Increasing surface FasL molecules and decreasing surface MHC-I molecules on infected CD4(+) cells send attacking cytotoxic CD8+ T-lymphocytes into apoptosis.; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike. Inhibits the Fas and TNFR-mediated death signals by blocking MAP3K5/ASK1. Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis. Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Extracellular Nef protein targets CD4(+) T-lymphocytes for apoptosis by interacting with CXCR4 surface receptors.
亞細胞定位：

Host cell membrane; Lipid-anchor; Cytoplasmic side. Virion. Secreted. Host Golgi apparatus membrane.
蛋白家族：

Lentivirus primate group Nef protein family