Recombinant Human cGMP-dependent 3',5'-cyclic phosphodiesterase (PDE2A), partial

中文名稱(chēng)：

人PDE2A重組蛋白
貨號(hào)：

CSB-YP517282HU
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱(chēng)：

人PDE2A重組蛋白
貨號(hào)：

CSB-EP517282HU
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱(chēng)：

人PDE2A重組蛋白
貨號(hào)：

CSB-EP517282HU-B
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱(chēng)：

人PDE2A重組蛋白
貨號(hào)：

CSB-BP517282HU
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱(chēng)：

人PDE2A重組蛋白
貨號(hào)：

CSB-MP517282HU
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

PDE2A
Uniprot No.：

O00408
別名：

5''-cyclic phosphodiesterase; cGMP dependent 3'5' cyclic phosphodiesterase; cGMP stimulated phosphodiesterase 1; cGMP stimulated phosphodiesterase 4; cGMP-dependent 3''; cGMP-stimulated phosphodiesterase 2; CGS PDE; CGS-PDE; cGSPDE; Cyclic GMP stimulated phosphodiesterase; Cyclic GMP-stimulated phosphodiesterase; Human cGMP stimulated 3'5' cyclic nucleotide phosphodiesterase; PDE 2A; PDE 2A1; PDE 2A4; PDE2A; PDE2A_HUMAN; PDE2A1; PDE2A4; phosphodiesterase 2A; Phosphodiesterase 2A cGMP stimulated
種屬：

Homo sapiens (Human)
蛋白長(zhǎng)度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Plays an important role in growth and invasion of malignant melanoma cells (e.g. pseudomyxoma peritonei (PMP) cell line).; Regulates mitochondrial cAMP levels and respiration. Involved in the regulation of mitochondria morphology/dynamics and apoptotic cell death via local modulation of cAMP/PKA signaling in the mitochondrion, including the monitoring of local cAMP levels at the outer mitochondrial membrane and of PKA-dependent phosphorylation of DNM1L.
基因功能參考文獻(xiàn)：
1. the well-known interaction between AIP and 2 different isoforms of phosphodiesterases (PDEs), PDE2A3 and PDE4A5, is of particular interest. While the interaction with over-expressed AIP does not seem to affect PDE2A3 function, the reported effect on PDE4A5 is, in contrast, reduced enzymatic activity. PMID: 28427099
2. Lu AF33241 represents a novel PDE2A/PDE10A inhibitor tool which can penetrate the blood brain barrier. PMID: 25941078
3. Epigenetic regulation of phosphodiesterases 2A and 3A underlies compromised beta-adrenergic Signaling in an induced pluripotent stem cell model of dilated cardiomyopathy. PMID: 26095046
4. curcumin exerts its in vitro anti-angiogenic and in vivo anti-tumour properties through combined PDE2 and PDE4 inhibition PMID: 25230992
5. PDE2A is involved in growth and invasion of human malignant melanoma PMP cells. PMID: 24705027
6. This study reports the X-ray crystal structure of PDE2A in complex with a highly selective, nanomolar inhibitor (BAY60-7550) at 1.9 A resolution, and the structure of apo PDE2 at 2.0 A resolution. PMID: 23899287
7. PDE2 overexpression in healthy cardiomyocytes reduces the rise in cyclic AMP levels and L-type calcium current amplitude, and abolishes the inotropic effect following acute beta-adrenergic receptor stimulation. PMID: 23810893
8. Our results demonstrated that the PDE2A2 variant carrying point mutations is expressed in PMP cells and may affect cell cycle progression by modulating cyclin A expression. PMID: 23381931
9. found in smooth muscle wall of blood vessels transversing the clitoral supepithelial and stromal space PMID: 21697861
10. Active site similarity between human and Plasmodium falciparum phosphodiesterases: considerations for antimalarial drug design PMID: 21766240
11. the phosphodiesterase 2A isoform localized to mitochondria regulates respiration PMID: 21724846
12. Inhibition of PDE2 resulted in higher levels of intracellular cAMP than inhibition of PDE3A suggesting this PDE may be the more important regulator of cAMP in human platelets. PMID: 12038792
13. TNF-alpha-mediated up-regulation of PDE2 may destabilize endothelial barrier function in sepsis PMID: 15650061
14. Phosphodiesterase 2 mediates redox-sensitive endothelial cell proliferation and angiogenesis by thrombin via Rac1 and NADPH oxidase 2. PMID: 19390057
15. report the X-ray crystal structures of a PDE protein that includes both catalytic and regulatory domains, namely, PDE2A containing the N-terminal GAF domains and the catalytic domain. PMID: 19828435
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亞細(xì)胞定位：

[Isoform PDE2A3]: Cell membrane; Lipid-anchor.; [Isoform PDE2A2]: Mitochondrion matrix. Mitochondrion inner membrane. Mitochondrion outer membrane.; [Isoform PDE2A1]: Cytoplasm.; [Isoform 5]: Mitochondrion.
蛋白家族：

Cyclic nucleotide phosphodiesterase family, PDE2 subfamily
組織特異性：

Expressed in brain and to a lesser extent in heart, placenta, lung, skeletal muscle, kidney and pancreas.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 8777

OMIM: 602658

KEGG: hsa:5138

STRING: 9606.ENSP00000334910

UniGene: Hs.503163