Recombinant Human Vimentin (VIM)

中文名稱：

人VIM重組蛋白
品名簡稱：

Recombinant Human VIM protein
貨號：

CSB-YP025857HU
說明書：

MSDS Datasheet
規格：

￥1500
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

VIM
Uniprot No.：

P08670
別名：

CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; VIM; VIME_HUMAN; Vimentin
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
來源：

Yeast
分子量：

55.5kDa
表達區域：

2-466aa
氨基酸序列

STRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

Recombinant Vimentin/VIM is a full-length of mature protein derived from the yeast cells. The expression region of this protein is the 2-466aa of human VIM protein. It carries an N-terminal 6xHis-tag and has a calculated molecular weight of 55.5 kDa. Its purity is greater than 90% determined by SDS-PAGE analysis. This recombinant VIM protein may be used to produce specific antibodies or in the studies of VIM-related cancer.

VIM is an intermediate filament (IF) helping to stabilize focal adhesion that governs cell migration. It also functions as a signal transducer from the extracellular matrix (ECM) to the nuclei. The VIM network recruits rapid phosphorylation and dephosphorylation that modulate integrin-mediated cell adhesion and facilitate directional cell motility. Pei-Wen Wang etc. demonstrated a new mechanism that VIM controlled the proliferation, differentiation, and movement of hepatic stellate cells (HSCs) through the ERK/AKT and Rho cascades. The study of Longxiang Su etc. revealed that VIM exerts a role in regulating immune cell apoptosis and inflammatory responses in sepsis.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.; Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
基因功能參考文獻：
1. This study measured the effects of vimentin expression on the mechano-elastic and migratory properties of the highly invasive breast carcinoma cell line MDA231. It demonstrated that vimentin stiffens cells and enhances cell migration in dense cultures, but exerts little or no effect on the migration of sparsely plated cells. PMID: 29022351
2. Positive vimentin expression could serve as a poor prognostic marker in gastric cancer[review, meta-analysis] PMID: 30078472
3. High vimentin expression is associated with pancreatic cancer. PMID: 29956814
4. the HDAC inhibitors augmented both Ecadherin and vimentin expression and their effects varied in different cholangiocarcinoma cell lines. Therefore, the clinical use of HDAC inhibitors in biliary cancer should be considered cautiously PMID: 29767267
5. miR-373 suppresses gastric cancer metastasis by downregulating vimentin. PMID: 29257346
6. Vimentin role in the M2BP inhibition of the HIV-1 virion production.M2BP mediates the interaction between HIV-1 Gag and Vimentin. PMID: 27604950
7. Desmin, Glial Fibrillary Acidic Protein, Vimentin, and Peripherin are type III intermediate filaments that have roles in health and disease [review] PMID: 29196434
8. Silencing of Vimentin in CNE2 cells leads to a decrease of microvessel density and VEGF, CD31, MMP2, and MMP9 expressions in pulmonary metastatic tumors. PMID: 28744809
9. Carcinoid-like/labyrinthine pattern of cell arrangement in vimentin/cytokeratin 20 expressing sebaceous neoplasms may represent a morphological phenotype of sebaceous mantles. PMID: 28027080
10. Cell surface vimentin mediates DENV-2 infection of vascular endothelial cells. PMID: 27910934
11. HIF-1alpha expression was upregulated in the vasculogenic mimicry-positive CRC cell line HCT-116 and thereby affected the expression of the EMT-related markers Claudin-4, E-cadherin (E-cd) and Vimentin(VIM). PMID: 27869227
12. our results demonstrated that vimentin in human GC tissues and cell lines was upregulated due to its de-ubiquitination after interactions with USP14 and miR-320a, which could promote the aggressiveness of GC cells. PMID: 27448976
13. Knocking down long pentraxin-3 (PTX3) or vimentin repressed oleate-induced head and neck squamous cell carcinomas (HNSCCs) invasion. PMID: 28489600
14. stromal vimentin expression is a promising indicator for survival prediction and adjuvant chemotherapy response in patients with stage II colorectal cancer with high-risk factors for recurrence. PMID: 28611349
15. Vimentin and its interaction with Shigella flexneri IpaC are dispensable for effector translocation pore formation, but are required for stable docking of Shigella flexneri to cells; moreover, stable docking triggers effector secretion. PMID: 27572444
16. the elongation reaction of vimentin in solution and in situ by time-resolved static and dynamic light scattering, is reported. PMID: 27655889
17. Vimentin induced by exosomes is necessary for lung cancer to induce mesenchymal transition (EMT) in recipient bronchial epithelial cells (HBECs). PMID: 27363026
18. our results demonstrate that vimentin silencing in ovarian cancer cells upregulates proteins of the exocytotic process to decrease cellular cisplatin accumulation PMID: 27322682
19. The combined biomarkers E-cadherin, membranous epidermal growth factor receptor (EGFR) and vimentin show a stronger prognostic value for and disease-free survival than any of the single biomarkers. PMID: 27172790
20. AHR protein-vimentin protein complex is formed in the cytoplasm resulting in proteasome degradation of vimentin. PMID: 27752740
21. TRIM56 is the ubiquitin ligase that is degrading vimentin in ovarian cancer cells, regulation cell migration and neoplasm invasiveness. PMID: 28771721
22. TIS21 attenuated Doxorubicin-induced cancer cell senescence by inhibiting linear actin nucleation via Nox4-ROS-ABI2-DRF signal cascade PMID: 27932314
23. this study indicates that OPN can induce epithelial-mesenchymal transition of hepatocellular carcinoma cells through increasing vimentin stability PMID: 26824421
24. The findings support a mechanism in which miR-375 suppresses RUNX1 levels, resulting in reduced vimentin and L-plastin expression. Knockdown of RUNX1, L-plastin, and vimentin resulted in significant reductions in cell invasion in vitro, indicating the functional significance of miR-375 regulation of specific proteins involved in head and neck squamous cell carcinoma (HNSCC) invasion. PMID: 28499703
25. Vimentin expression was an adverse prognostic factor for DSS in TSCC patients, even after the adjustment for cell differentiation, pathological stage, and expression levels of Snail, Twist, E-cadherin, and N-cadherin. Snail, E-cadherin, N-cadherin, and Vimentin were associated with tumorigenesis and pathological outcomes PMID: 28570699
26. circulating anti-vimentin IgG autoantibody levels are much greater in idiopathic pulmonary fibrosis subjects than in normal controls PMID: 28754682
27. RhoA/ROCK and Raf-1/CK2 pathway are responsible for TNF-alpha-mediated endothelial cytotoxicity via regulation of the vimentin cytoskeleton. PMID: 28743511
28. It was concluded that islet cell expression of vimentin indicates a degree of plasticity and dedifferentiation with potential loss of cellular identity in diabetes. PMID: 28348116
29. findings have identified a role for members of these signaling pathways in the regulation of EGF-induced vimentin expression in the MDA-MB-468 breast cancer cell line PMID: 27163529
30. changes in methylation levels in cfDNA associated with hepatocellular carcinoma and could represent useful plasma-based biomarker PMID: 28333958
31. vimentin regulates the differentiation switch via modulation of K5/K14 expression. Moreover, because there was a significant correlation between high vimentin-K14 expression and recurrence/poor survival in oral cancer patients, vimentin-K14 together may prove to be the novel markers for the prognostication of human oral cancer. PMID: 28225793
32. Data indicate that ellagic aicd (EA) down-regulates the expression of COX-2, NF-kappa B, vimentin and up-regulates the expression of E-cadherin in in pancreatic carcinom PANC-1 cells. PMID: 28135203
33. Bevacizumab treatment was also associated with structural protein abnormalities, with decreased GFAP and vimentin content and upregulated GFAP and vimentin mRNA expression. PMID: 28419863
34. Decreasing cell surface vimentin by small interfering RNA (siRNA) knockdown in HeLa and NIKS cells significantly increased human papillomavirus 16 infectious internalization, while overexpression of vimentin had the opposite effect identifying vimentin as a viral restriction factor. PMID: 28566373
35. From these data, we suggest that filamentous vimentin underneath the plasma membrane is involved in increasing integrin adhesiveness, and thus regulation of the vimentin-integrin interaction might control cell adhesion PMID: 27044755
36. depletion induces phosphorylation of the microtubule-associated GEF-H1 on Ser886, and thereby promotes RhoA activity and actin stress fiber assembly. PMID: 28096473
37. Data show that the filament elongation of both desmin and keratin K8/K18 proceeds very similar to that of vimentin. PMID: 27304995
38. Coexistence of vimentin-positive and Axl-high expression is a poor prognostic factor for primary breast cancer. Vimentin and Axl expression might contribute to the aggressive phenotype in breast cancer. PMID: 27506606
39. our findings suggest that the HIF-1alpha-HDAC1 complex transcriptionally inhibits miR-548an expression during hypoxia, resulting in the upregulation of vimentin that facilitates the pancreatic tumorigenesis. PMID: 27353169
40. Rab7a depletion decreases the amount of active Rac1 but not its abundance and reduces the number of cells with vimentin filaments facing the wound, indicating that Rab7a has a role in the orientation of vimentin filaments during migration PMID: 27888097
41. Over-expression of TNC, SMA, and vimentin were significantly correlated with the lower overall survival in prostate cancer patients. PMID: 28341124
42. Results show that VIM mRNA is regulated in melanoma by UNR protein. PMID: 27908735
43. These findings suggest that Plk1 regulates smooth muscle contraction by modulating vimentin phosphorylation at Ser-56. PMID: 27662907
44. Structural Dynamics of the Vimentin Coiled-coil Contact Regions Involved in Filament Assembly as Revealed by Hydrogen-Deuterium Exchange.( PMID: 27694444
45. These findings are the first to demonstrate that vimentin is critical for influenza viral infection as it facilitates endosomal trafficking and acidification, and mediates viral genome penetration into the cytoplasm to propagate the infection. PMID: 27423069
46. RAP1 promotes colorectal cell migration through the regulation of Vimentin and RAP1 may act as a potential target for the diagnosis and therapy of CRC. PMID: 28381157
47. High vimentin expression is associated with Non Small-cell Lung Cancer. PMID: 28373440
48. data demonstrates that keratinocyte migration requires the interaction between vimentin and keratins at the -YRKLLEGEE- sequence at the helical 2B domain of vimentin PMID: 27072292
49. High vimentin expression is associated with malignant pleural mesothelioma. PMID: 27646775
50. Increased TET1 induced re-expression of vimentin through active DNA demethylation, and cause partial epithelial-to-mesenchymal (EMT) in A2780 cells. PMID: 28150354
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相關疾病：

Cataract 30, multiple types (CTRCT30)
亞細胞定位：

Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix. Cell membrane.
蛋白家族：

Intermediate filament family
組織特異性：

Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
數據庫鏈接：

HGNC: 12692

OMIM: 116300

KEGG: hsa:7431

STRING: 9606.ENSP00000224237

UniGene: Hs.455493