Recombinant Human Ubiquitin-conjugating enzyme E2 O (UBE2O), partial

中文名稱：

人UBE2O重組蛋白
貨號：

CSB-YP866339HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人UBE2O重組蛋白
貨號：

CSB-EP866339HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人UBE2O重組蛋白
貨號：

CSB-EP866339HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人UBE2O重組蛋白
貨號：

CSB-BP866339HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人UBE2O重組蛋白
貨號：

CSB-MP866339HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

UBE2O
Uniprot No.：

Q9C0C9
別名：

E2 230K; EC=6.3.2.19; FLJ12878; KIAA1734; Likely Ortholog of Mouse Ubiquitin Conjugating Enzyme; RP23 193A16.5; UBE2O; UBE2O_HUMAN; Ubiquitin carrier protein O; Ubiquitin conjugating enzyme E2 O; Ubiquitin conjugating enzyme E2 of 230 kDa; ubiquitin conjugating enzyme E2O; Ubiquitin protein ligase O; Ubiquitin-conjugating enzyme E2 O; Ubiquitin-conjugating enzyme E2 of 230 kDa; Ubiquitin-conjugating enzyme E2-230K; Ubiquitin-protein ligase O
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins. Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity. Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis. Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location. Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly.
基因功能參考文獻：
1. UBE2O is a self-contained quality control factor that comprises substrate recognition and ubiquitin transfer activities within a single protein to efficiently target orphans of multiprotein complexes for degradation. PMID: 28774922
2. UBE2O mediates c-Maf polyubiquitination and degradation, induces MM cell apoptosis, and suppresses myeloma tumor growth, which provides a novel insight in understanding myelomagenesis and UBE2O biology. PMID: 28673317
3. UBE2O specifically targets AMPKalpha2 for ubiquitination and degradation, and thereby promotes activation of the mTOR-HIF1alpha pathway. PMID: 28162974
4. UBE2O defines an atypical ubiquitin-signaling pathway that coordinates the function of BAP1. PMID: 24703950
5. UBE2O negatively regulates TRAF6-mediated NF-kappaB activation by inhibiting TRAF6 polyubiquitination. PMID: 23381138
6. monoubiquitinated SMAD6 impairs the binding affinity of non-modified SMAD6 to the BMP type I receptor. Moreover, UBE2O and SMAD6 cooperated in the regulation of BMP7-induced adipogenesis PMID: 23455153
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亞細胞定位：

Cytoplasm. Nucleus. Note=Mainly localizes to the cytoplasm.
蛋白家族：

Ubiquitin-conjugating enzyme family
組織特異性：

Predominantly expressed in skeletal muscle and heart.
數據庫鏈接：

HGNC: 29554

OMIM: 617649

KEGG: hsa:63893

STRING: 9606.ENSP00000323687

UniGene: Hs.16130