Recombinant Human mRNA decay activator protein ZFP36 (ZFP36)
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中文名稱：人ZFP36重組蛋白
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貨號：CSB-EP026448HU-B
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說明書：
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規格：
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來源：E.coli
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共軛：Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他：
產品詳情
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純度：>85% (SDS-PAGE)
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基因名：ZFP36
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Uniprot No.：
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別名：G0/G1 switch regulatory protein 24; G0S24; GOS24; Growth factor-inducible nuclear protein NUP475; NUP475; Protein TIS11A; RNF162A; TIS 11; TIS11; TIS11A; Tristetraprolin; Tristetraproline; TTP; TTP_HUMAN; Zfp-36; ZFP36; Zinc finger protein 36; Zinc finger protein 36 homolog; Zinc finger protein 36, C3H type, homolog (mouse); Zinc finger protein, C3H type, 36 homolog
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種屬：Homo sapiens (Human)
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蛋白長度：Full length protein
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表達區域：1-326
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氨基酸序列MDLTAIYESL LSLSPDVPVP SDHGGTESSP GWGSSGPWSL SPSDSSPSGV TSRLPGRSTS LVEGRSCGWV PPPPGFAPLA PRLGPELSPS PTSPTATSTT PSRYKTELCR TFSESGRCRY GAKCQFAHGL GELRQANRHP KYKTELCHKF YLQGRCPYGS RCHFIHNPSE DLAAPGHPPV LRQSISFSGL PSGRRTSPPP PGLAGPSLSS SSFSPSSSPP PPGDLPLSPS AFSAAPGTPL ARRDPTPVCC PSCRRATPIS VWGPLGGLVR TPSVQSLGSD PDEYASSGSS LGGSDSPVFE AGVFAPPQPV AAPRRLPIFN RISVSE
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蛋白標簽：Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產品提供形式：Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復溶：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件：Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期：The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期：Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項：Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet ：Please contact us to get it.
產品評價
相關產品
靶點詳情
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功能：Zinc-finger RNA-binding protein that destabilizes several cytoplasmic AU-rich element (ARE)-containing mRNA transcripts by promoting their poly(A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis. Acts as an 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery. Recruits deadenylase CNOT7 (and probably the CCR4-NOT complex) via association with CNOT1, and hence promotes ARE-mediated mRNA deadenylation. Functions also by recruiting components of the cytoplasmic RNA decay machinery to the bound ARE-containing mRNAs. Self regulates by destabilizing its own mRNA. Binds to 3'-UTR ARE of numerous mRNAs and of its own mRNA. Plays a role in anti-inflammatory responses; suppresses tumor necrosis factor (TNF)-alpha production by stimulating ARE-mediated TNF-alpha mRNA decay and several other inflammatory ARE-containing mRNAs in interferon (IFN)- and/or lipopolysaccharide (LPS)-induced macrophages. Plays also a role in the regulation of dendritic cell maturation at the post-transcriptional level, and hence operates as part of a negative feedback loop to limit the inflammatory response. Promotes ARE-mediated mRNA decay of hypoxia-inducible factor HIF1A mRNA during the response of endothelial cells to hypoxia. Positively regulates early adipogenesis of preadipocytes by promoting ARE-mediated mRNA decay of immediate early genes (IEGs). Negatively regulates hematopoietic/erythroid cell differentiation by promoting ARE-mediated mRNA decay of the transcription factor STAT5B mRNA. Plays a role in maintaining skeletal muscle satellite cell quiescence by promoting ARE-mediated mRNA decay of the myogenic determination factor MYOD1 mRNA. Associates also with and regulates the expression of non-ARE-containing target mRNAs at the post-transcriptional level, such as MHC class I mRNAs. Participates in association with argonaute RISC catalytic components in the ARE-mediated mRNA decay mechanism; assists microRNA (miRNA) targeting ARE-containing mRNAs. May also play a role in the regulation of cytoplasmic mRNA decapping; enhances decapping of ARE-containing RNAs, in vitro. Involved in the delivery of target ARE-mRNAs to processing bodies (PBs). In addition to its cytosolic mRNA-decay function, affects nuclear pre-mRNA processing. Negatively regulates nuclear poly(A)-binding protein PABPN1-stimulated polyadenylation activity on ARE-containing pre-mRNA during LPS-stimulated macrophages. Also involved in the regulation of stress granule (SG) and P-body (PB) formation and fusion. Plays a role in the regulation of keratinocyte proliferation, differentiation and apoptosis. Plays a role as a tumor suppressor by inhibiting cell proliferation in breast cancer cells.; (Microbial infection) Negatively regulates HTLV-1 TAX-dependent transactivation of viral long terminal repeat (LTR) promoter.
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基因功能參考文獻：
- Overexpression of miRNA-29c in podocytes resulted in an increase in inflammatory cytokines and inhibition of miRNA-29c by using its inhibitor reduced the inflammatory cytokines in podocytes. Finally, miRNA-29c promoted the progression of diabetic nephropathy (DN) by targeting TTP, providing a target for a therapeutic intervention of DN. PMID: 28539664
- Data indicate that the angiopoietin-like protein 4 (ANGPTL4)-mediated upregulation of tristetraprolin expression regulates the stability of chemokines in human colon epithelial cells. PMID: 28287161
- PD-L1 is a critical TTP-regulated factor that contributes to inhibiting antitumor immunity. PMID: 29936792
- The role of TTP in human cancers and immunity.[review] PMID: 29124478
- Findings reveal tristetraprolin, which is known to bind COX-2 mRNA and promote its rapid degradation, as regulator of COX-2 expression in influenza A viruses (IAV) infection. During IAV replication and viral RNA accumulation TTP mRNA synthesis was induced, resulting in reduced COX-2 levels. PMID: 27265729
- our data demonstrate that TTP acts as a regulator of mitochondrial dynamics through enhancing degradation of alpha-Syn mRNA in cancer cells. This finding will increase understanding of the molecular basis of mitochondrial dynamics. PMID: 28410208
- Suggest that TTP is an important prognostic indicator for prostate cancer, and augmenting TTP function would effectively disable the metabolism and proliferation of aggressive prostate tumors. PMID: 27825143
- RAS signaling can upregulate tumor cell PD-L1 expression through a mechanism involving increases in PD-L1 mRNA stability via modulation of the AU-rich element-binding protein tristetraprolin. PMID: 29246442
- Because TTP also represses IL-1beta expression, it is a dual inhibitor of the IL-1beta system, regulating expression of the cytokine and the upstream controller NLRP3 PMID: 28302726
- Data show that the synthetic liver X receptors (LXRs) agonist T0901317 promoted cytokines IL-1beta, IL-6 and TNFalpha mRNA degradation, destabilized TNFalpha mRNA through its 3'-untranslated region, and increased the expression of tristetraprolin (TTP). PMID: 28119310
- TTP was demonstrated to destabilize the VEGF mRNA in ARPE-19 cells under hypoxic conditions. Furthermore, CM from TTP-overexpressing ARPE-19 cells suppressed tube formation in HUVECs. PMID: 27840917
- this study shows that TTP is down-regulated in glioma tissue samples and cell lines, and is associated with diminished survival in glioma patients PMID: 27424080
- TTP inhibited the proliferation, migration, and invasion of gastric cancer cell lines through regulation of IL-33. PMID: 27074834
- Results suggest that ZFP36 proteins might control reepithelialization and angiogenesis in the skin in a multimodal manner. PMID: 27182009
- Our data offer the convincing evidence for the first time that the aberrant expressions of ZFP36 and SOCS3 may be involved into the progression and patients' prognosis of prostate cancer PMID: 26563146
- Low TTP expression is associated with epithelial-mesenchymal transition in cancer. PMID: 26840564
- PP2A activators boost the anti-inflammatory function of TTP and have implications for future pharmacotherapeutic strategies to combat inflammation in respiratory disease. PMID: 26820662
- The mRNA destabilizing activity of TTP was increased when the partially disordered RNA binding domain (RBD) of TTP was replaced with the fully structured RBD of TIS11d, indicating that differences in the folded state of the RBD affect the activity of the proteins in the cell. PMID: 26551835
- Tristetraprolin induces cell cycle arrest in breast tumor cells by targeting AP-1/c-Jun and NF-kappaB/RelA pathway. PMID: 26497679
- TTP-dependent regulatory pathway described herein likely contributes to the role of IL-22 in inflammation and cancer and may evolve as novel target for pharmacological IL-22 modulation. PMID: 26486958
- Results suggest that miR-29a acts as an oncogene by down regulating TTP and suggest the potential of miR-29a and TTP as biomarkers. PMID: 26356262
- TTP might regulate interleukin (IL)-6 and IL-18 expression in diabetes. Diabetes with clinical proteinuria is accompanied by decreased urinary and serum level of TTP mRNA and protein, and increased levels of IL-6 and IL-18 mRNA and protein. PMID: 26517838
- Involvement of LARP4 as a target of TNF-alpha-TTP regulation provides a clue as to how its functional activity may be used in a physiologic pathway. PMID: 26644407
- Modulation of TAFI levels by pro-inflammatory mediators or lipopolysaccharide involves binding of tristetraprolin to the CPB2 3'-UTR, which mediates CPB2 mRNA destabilisation. PMID: 26062599
- DUSP1 overexpression and inhibition of MAPKs prevented IL1B-induced expression of ZFP36, this was associated with increased TNF mRNA expression at 6 h, an effect that was predominantly due to elevated transcription. PMID: 26546680
- ZFP36 preferentially binds to 3' UTRs of mRNAs encoding regulators of gene expression. PMID: 24401661
- the biphasic nature of TNF-alpha-induced IL-6 mRNA expression was regulated temporally by the RNA-destabilizing molecule, TTP. PMID: 25724669
- Logistic regression analyses demonstrated that TNF-alpha and the three ZFP36 gene polymorphisms were not independently associated with obstructive sleep apnea. PMID: 26125882
- Low levels of TTP is associated with cisplatin-resistance in head and neck cancer. PMID: 25604244
- ZFP36 controls RIP1 levels in glioma neural stem cell lines. PMID: 25939870
- In this review we summarize current progress regarding the specific characteristics of sequences and structures in the 3' untranslated regions of mRNAs that are recognized by tristetraproline, Roquins, and Regnase-1. PMID: 25955820
- Tristetraprolin (TTP) recruits eukaryotic initiation factor 4E2 (eIF4E2) to repress target mRNA translation. PMID: 26370510
- TGF-beta decreases the stability of IL-18-induced IFN-gamma mRNA through the expression of TGF-beta-induced tristetraprolin in KG-1 cells PMID: 25832634
- The abnormalities were screened by FISH in 44 epithelioid hemangioma (EH) from different locations with seven additional EH revealing FOSB gene rearrangements, all except one being fused to ZFP36. PMID: 25043949
- TTP mediates the cleavage of the 3' UTRs of stress response mRNAs by recruiting the HSV-1 VHS RNase to the AU-rich elements. PMID: 25762736
- Tristetraprolin is involved in the glucocorticoid-mediated interleukin 8 repression PMID: 25111853
- CNOT7/hCAF1 is involved in ICAM-1 and IL-8 regulation by TTP in HPMEC. PMID: 25038453
- Inactivation of tristetraprolin in chronic hypoxia provokes the expression of cathepsin B PMID: 25452305
- An association with Graves ophthalmopathy was shown with two SNPs in ZFP36. PMID: 24780075
- hnRNP F is a co-factor in a subset of tristetraprolin/BRF1/BRF2-mediated mRNA decay. PMID: 24978456
- Data indicate tristetraprolin (TTP) promotes apoptosis of HeLa cervical cancer cells in the presence of tumor necrosis factor. PMID: 25056949
- These results demonstrate that E2F1 mRNA is a physiological target of TTP and suggests that TTP controls proliferation as well as migration and invasion through the regulation of E2F1 mRNA stability. PMID: 24150491
- TTP acts as a bona fide ERalpha corepressor and suggest that this protein may be a contributing factor in the development of E2-dependent tumors in breast cancer. PMID: 24737323
- although the majority of conserved residues within the TZF domain of TTP are required for productive binding, not all residues at sequence-equivalent positions in the two zinc fingers of the TZF domain of TTP are functionally equivalent. PMID: 24253039
- Reduced tristetraprolin function through phosphorylation is associated with malignant glioma. PMID: 23525947
- ZFP36 gene is not associated with HDL-C in Uygur people from Xinjiang PMID: 23744329
- Loss of TTP promotes invasion in head and neck cancer via transcript stabilization and secretion of MMP9, MMP2, and IL-6. PMID: 23349315
- These results suggested that during LPS stimulation, NF-kappaB signaling were activated to regulate the transcription of TTP mRNA. PMID: 23212617
- Authors identify an evolutionarily conserved C-terminal motif in human TTP that directly binds a central domain of CNOT1, a core subunit of the CCR4-NOT complex. PMID: 23644599
- Tristetraprolin provides an important link between p53 activation induced by DNA damage and let-7 biogenesis. PMID: 23595149
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亞細胞定位：Nucleus. Cytoplasm. Cytoplasmic granule. Cytoplasm, P-body.; Nucleus. Cytoplasm.
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組織特異性：Expressed in both basal and suprabasal epidermal layers. Expressed in epidermal keratinocytes. Expressed strongly in mature dendritic cells. Expressed in immature dendritic cells (at protein level).
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