Recombinant Human Tapasin (TAPBP), partial

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中文名稱：

人TAPBP重組蛋白
品名簡稱：

Recombinant Human TAPBP protein, partial
貨號：

CSB-EP023122HU1
說明書：

MSDS Datasheet
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

TAPBP
Uniprot No.：

O15533
別名：

(TPN)(TPSN)(NGS-17)(TAP-associated protein)(TAP-binding protein)
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
來源：

E.coli
分子量：

49.4 kDa
表達區域：

21-414aa
氨基酸序列

GPAVIECWFVEDASGKGLAKRPGALLLRQGPGEPPPRPDLDPELYLSVHDPAGALQAAFRRYPRGAPAPHCEMSRFVPLPASAKWASGLTPAQNCPRALDGAWLMVSISSPVLSLSSLLRPQPEPQQEPVLITMATVVLTVLTHTPAPRVRLGQDALLDLSFAYMPPTSEAASSLAPGPPPFGLEWRRQHLGKGHLLLAATPGLNGQMPAAQEGAVAFAAWDDDEPWGPWTGNGTFWLPTVQPFQEGTYLATIHLPYLQGQVTLELAVYKPPKVSLMPATLARAAPGEAPPELLCLVSHFYPSGGLEVEWELRGGPGGRSQKAEGQRWLSALRHHSDGSVSLSGHLQPPPVTTEQHGARYACRIHHPSLPASGRSAEVTLEVAGLSGPSLEDSV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).
基因功能參考文獻：
1. The results demonstrated that the mRNA expression levels of tapasin were significantly downregulated in patients with chronic hepatitis B (CHB) compared with in healthy controls and patients with acute hepatitis B Furthermore, the apoptotic rate of CD8+ T cells was increased in patients with CHB compared with in the other two groups. PMID: 27510296
2. Fluctuation and entropy analyses show how tapasin chaperones major histocompatibility complex class I by stabilising it in a peptide-receptive conformation. PMID: 26754481
3. reduction in tapasin expression is associated with tumor progression in colorectal cancer PMID: 26310568
4. Data suggest that tapasin (Tsn) binds to MHC I with suboptimal cargo and thereby adjusts the energy landscape in favor of MHC I complexes with immunodominant epitopes. PMID: 26416272
5. Analysis of expression of tapasin and/or HLA-I may be of value as prognostic tool for glioblastoma multiforme patients, especially when considering immunotherapy. PMID: 25175688
6. Modified TAPBP gene function may contribute to the development of refractory chronic rhinosinusitis via reduction of circulating CD8 lymphocytes. PMID: 23640800
7. Targeted re-sequencing identified rs3106189 at the 5' UTR of TAPBP and rs1052918 at the 3' UTR of TCF3 to be associated with the overall survival of colorectal cancer patients. PMID: 23940558
8. the data indicate that TAPBPR and tapasin bind in a similar orientation to the same face of MHC class I. PMID: 24163410
9. TAPBP polymorphisms may play a role in the development of aspirin-exacerbated respiratory disease. PMID: 23736108
10. isoform lacking exon 3 affects MHC class I-peptide binding PMID: 23519916
11. Data indicate that the peptide-loading complex (PLC) consists maximally of 2x tapasin-ERp57/MHC I per TAP complex, but one tapasin-ERp57/MHC I in the PLC is essential and sufficient for antigen processing. PMID: 22923333
12. Tapasin discriminates peptide-human leukocyte antigen-A*02:01 complexes formed with natural ligands. PMID: 21518758
13. On infection with human cytomegalovirus tapasin mRNA levels were continuously downregulated during infection, while tapasin transcripts remained stable and long-lived. PMID: 21248040
14. Downregulation of tapasin is associated with a poor clinical outcome for oral squamous cell carcinoma patients and may serve as a prognostic biomarker PMID: 20532727
15. The interactions of tapasin with both TAP and ERp57 are correlated with strong MHC class I recruitment and assembly enhancement. PMID: 20070606
16. find that in a competitive situation between high- and low-affinity peptides, tapasin mediates the binding of the high-affinity peptide to class I by accelerating the dissociation of the peptide from an unstable intermediate of the binding reaction. PMID: 20017190
17. recruits MHC class I molecules to TAP complex during antigen processing PMID: 11823531
18. tapasin is a modified Mhc class I molecule PMID: 11862402
19. MHC class I molecules can optimize their peptide repertoire over time and that this process is dependent on tapasin. PMID: 11970875
20. tapasin is not required for calreticulin to bind to the alpha1 domain of MHC class I molecules PMID: 11972874
21. results suggest that tapasin deficiency is another cause of type I bare lymphocyte syndrome PMID: 12149238
22. Tapasin enhances the structural stability of TAP1.TAP2 complexes. PMID: 12213826
23. The domain organization of tapasin is an assembly of two core regions of different sizes loosely connected by a linker or loop comprising residues ~85-93. PMID: 12463753
24. tapasin has a essential function of tapasin in quality control of HLA-G molecules PMID: 12582157
25. A major role for tapasin as a stabilizer of the TAP peptide transporter and consequences for MHC class I expression. PMID: 12594855
26. Downregulation of tapasin in advanced stages of human melanoma may reflect accumulation of alterations in antigen-presenting/processing machinery associated with neoplastic progression. May contribute to immune escape phenotype of human melanoma cells. PMID: 12682852
27. The ERp57-tapasin conjugate can also be modified with the oxidizing agent diamide, indicating that within the pool of ERp57-tapasin complexes the free, non-tapasin-linked CXXC motif exists in both oxidized and reduced states PMID: 13678524
28. Defects in tapasin and HLA class I antigen expression in primary maxillary sinus SCC lesions may play a role in the clinical course of the maxillary sinus cancer, because these defects were associated with poor prognosis. PMID: 14519625
29. In its role as peptide facilitator, tapasin stabilizes the peptide-free conformation of class I major histocompatibility (MHC) complex molecules in the endoplasmic reticulum and thus increases the number and variety of peptides bound to class I MHC. PMID: 14607930
30. Mutational analysis of tapasin provides insight into aspects of tapasin structure that are crucial to its ability to assist major histocompatibility complex class I assembly. PMID: 14978101
31. Transfection of tapasin into the Panc02 cells did not quantitatively increase MHC class I surface expression or detectably affect MHC class I association with tumor-specific peptides and beta(2)-microglubulin (beta(2)m). PMID: 15163903
32. Defective tapasin transcription and thus absence of HLA-B44 expression is associateed with colorectal tumors. PMID: 15455354
33. Substitutions at position lysine-408 in tapasin are shown to affect the expression of major histocompatibility complex class I molecules at the cell surface, by down-regulating tapasin stabilization of TAP. PMID: 15634919
34. Tapasin association specifically inhibits the escape pathway required for disulfide-bond isomerization within conventional protein substrates, suggesting a specific structural role for ERp57 within the MHC class I peptide-loading complex. PMID: 16193070
35. Down-regulation of tapasin expression was associated with glioblastoma multiforme PMID: 16322289
36. Besides beta(2)m and tapasin, an extra unidentified component is also critical for the expression of certain human class I alleles. PMID: 17498802
37. A ternary complex between heavy chain, ERp57, and tapasin was observed and shown to be stabilized by a disulfide between both tapasinheavy chain and tapasin-ERp57. PMID: 18039656
38. Transgenic tapasin establishes hierarchical responses in vivo according to peptide-major histocompatibility complex class I stability. PMID: 18196518
39. In this review, interaction of accessory protein tapasin with HLA-DM crucially influences the selection of peptides that bind to major histocompatibility complex (MHC) molecules during antigen presentation. PMID: 18261958
40. the 2.6 A resolution structure of the tapasin-ERp57 core of the peptide-loading complex PMID: 19119025
41. HLA-B27 polymorphism drives the tapasin dependency, rates of intracellular maturation and expressions of homodimers. PMID: 19167761
42. tapasin conjugation with ERp57 is as critical as its integration into the membrane for efficient MHC class I assembly, surface expression, and Ag presentation to CD8+ T cells. PMID: 19701894
43. The N-terminal region of Tapasin (Tpn) can be recombinantly expressed and adopt a structure, which at least partially resembles that of wild-type Tpn. This region of Tpn features chaperone activity facilitating peptide binding of MHC-I. PMID: 19728311
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相關疾病：

Bare lymphocyte syndrome 1 (BLS1)
亞細胞定位：

Endoplasmic reticulum membrane; Single-pass type I membrane protein.
組織特異性：

Neutrophils, mostly in fully differentiated cells.
數據庫鏈接：

HGNC: 11566

OMIM: 601962

KEGG: hsa:6892

STRING: 9606.ENSP00000404833

UniGene: Hs.370937