Recombinant Human Rho GTPase-activating protein 1 (ARHGAP1)

貨號(hào)：

CSB-YP691829HU
規(guī)格：
來(lái)源：

Yeast
其他：

貨號(hào)：

CSB-EP691829HU
規(guī)格：
來(lái)源：

E.coli
其他：

貨號(hào)：

CSB-EP691829HU-B
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

貨號(hào)：

CSB-BP691829HU
規(guī)格：
來(lái)源：

Baculovirus
其他：

貨號(hào)：

CSB-MP691829HU
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

ARHGAP1
Uniprot No.：

Q07960
別名：

Rho GTPase-activating protein 1; CDC42 GTPase-activating protein; GTPase-activating protein rhoGAP; Rho-related small GTPase protein activator; Rho-type GTPase-activating protein 1; p50-RhoGAP; ARHGAP1 CDC42GAP RHOGAP1
種屬：

Homo sapiens (Human)
蛋白長(zhǎng)度：

Full Length
表達(dá)區(qū)域：

1-439
氨基酸序列

MDPLSELQDDLTLDDTSEALNQLKLASIDEKNWPSDEMPDFPKSDDSKSSSPELVTHLKWDDPYYDIARHQIVEVAGDDKYGRKIIVFSACRMPPSHQLDHSKLLGYLKHTLDQYVESDYTLLYLHHGLTSDNKPSLSWLRDAYREFDRKYKKNIKALYIVHPTMFIKTLLILFKPLISFKFGQKIFYVNYLSELSEHVKLEQLGIPRQVLKYDDFLKSTQKSPATAPKPMPPRPPLPNQQFGVSLQHLQEKNPEQEPIPIVLRETVAYLQAHALTTEGIFRRSANTQVVREVQQKYNMGLPVDFDQYNELHLPAVILKTFLRELPEPLLTFDLYPHVVGFLNIDESQRVPATLQVLQTLPEENYQVLRFLTAFLVQISAHSDQNKMTNTNLAVVFGPNLLWAKDAAITLKAINPINTFTKFLLDHQGELFPSPDPSGL
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate.
基因功能參考文獻(xiàn)：
1. identified ARHGAP1, which is a negative regulator of CDC42, as a novel, direct target of miR-130b PMID: 28748534
2. to complete invasion of the endothelium, staphylococci reorient recycling endocytic vesicles to recruit Cdc42GAP. PMID: 27311480
3. we identified novel associations in WLS , ARHGAP1 , and 5' of MEF2C ( P- values < 8x10 - 5 ; false discovery rate (FDR) q-values < 0.01) that were much more strongly associated with BMD compared to the GWAS SNPs. PMID: 27616567
4. study shows that HMHA1 acts as a RhoGAP to regulate GTPase activity, cytoskeletal remodeling and cell spreading, which are crucial functions in normal hematopoietic and cancer cells PMID: 24086303
5. The interaction of RhoGap protein stabilizes the tetrameric conformation of p53 and enhances its DNA-binding activity, thereby inducing cell-cycle arrest and apoptosis. PMID: 23684608
6. In PC3 cells, ARHGAP21 presented GAP activity for RhoA and RhoC and induced changes in cell morphology. Moreover, its silencing altered the expression of genes involved in cell proliferation and cytoskeleton organization PMID: 23200924
7. RhoGAP Stard13 temporally and spatially regulates Rho activity in the developing pancreas to ensure that morphogenesis and establishment of tissue architecture are coordinated with organ growth. PMID: 23175628
8. Rho proteins and ROK play a pivotal role in this signaling cascade PMID: 21051874
9. PA and Rap1 cooperatively regulate RA-RhoGAP activity for promoting neurite outgrowth. PMID: 21169361
10. Mutation of three residues of CDC42GAP involved in specific interactions of CDC42GAP with switch domain residues of Cdc42, as well as individual mutations of each of these residues, yields GAPs that are defective in stimulating GTP hydrolysis. PMID: 12501193
11. phosphorylation of Y1214 on VEGFR2 is required to trigger the sequential activation of Cdc42 and SAPK2/p38 and to drive the SAPK2/p38-mediated actin remodeling in stress fibers in endothelial cells exposed to VEGF PMID: 14724572
12. lipopolysaccharide-induced activation of the Rho family GTPase Cdc42, and we show its activation in the human neutrophil to be mediated by a p38 mitogen-activated protein kinase-dependent mechanism. PMID: 15262974
13. CDC42GAP was identified as a counter-regulatory mediator for tubule formation. PMID: 18060510
14. The NR5A1 gene expression was activated in mesenchymal stem cells by siRNA directed against RhoGAP (ARHGAP26). PMID: 18579725
15. two potential interaction partners of Prx6: the calcium-activated cysteine endopeptidase calpain and the p50RhoGAP protein of the family of Sec14-like proteins. PMID: 18619034
16. Results show that phorbol-ester-induced activation of protein kinases C and D stimulates association of DLC1 with 14-3-3 adaptor proteins, inhibiting RhoGAP activity and blocking nucleocytoplasmic transfer. PMID: 19066281
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亞細(xì)胞定位：

Cytoplasm.
組織特異性：

Ubiquitous.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 673

OMIM: 602732

KEGG: hsa:392

STRING: 9606.ENSP00000310491

UniGene: Hs.138860