Recombinant Human Nexilin (NEXN)

中文名稱：

人NEXN重組蛋白
貨號：

CSB-YP605897HU
規(guī)格：
來源：

Yeast
其他：

中文名稱：

人NEXN重組蛋白
貨號：

CSB-EP605897HU
規(guī)格：
來源：

E.coli
其他：

中文名稱：

人NEXN重組蛋白
貨號：

CSB-EP605897HU-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人NEXN重組蛋白
貨號：

CSB-BP605897HU
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

人NEXN重組蛋白
貨號：

CSB-MP605897HU
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

NEXN
Uniprot No.：

Q0ZGT2
別名：

1110046H09Rik; AA553326; F-actin binding protein ; F-actin-binding protein; MGC104234; MGC138865; MGC138866; Nelin; Nexilin; Nexn; NEXN_HUMAN; nF actin binding protein; OTTHUMP00000038336
種屬：

Homo sapiens (Human)
蛋白長度：

full length protein
表達(dá)區(qū)域：

1-675
氨基酸序列

MNDISQKAEI LLSSSKPVPK TYVPKLGKGD VKDKFEAMQR AREERNQRRS RDEKQRRKEQ YIREREWNRR KQEIKEMLAS DDEEDVSSKV EKAYVPKLTG TVKGRFAEME KQRQEEQRKR TEEERKRRIE QDMLEKRKIQ RELAKRAEQI EDINNTGTES ASEEGDDSLL ITVVPVKSYK TSGKMKKNFE DLEKEREEKE RIKYEEDKRI RYEEQRPSLK EAKCLSLVMD DEIESEAKKE SLSPGKLKLT FEELERQRQE NRKKQAEEEA RKRLEEEKRA FEEARRQMVN EDEENQDTAK IFKGYRPGKL KLSFEEMERQ RREDEKRKAE EEARRRIEEE KKAFAEARRN MVVDDDSPEM YKTISQEFLT PGKLEINFEE LLKQKMEEEK RRTEEERKHK LEMEKQEFEQ LRQEMGEEEE ENETFGLSRE YEELIKLKRS GSIQAKNLKS KFEKIGQLSE KEIQKKIEEE RARRRAIDLE IKEREAENFH EEDDVDVRPA RKSEAPFTHK VNMKARFEQM AKAREEEEQR RIEEQKLLRM QFEQREIDAA LQKKREEEEE EEGSIMNGST AEDEEQTRSG APWFKKPLKN TSVVDSEPVR FTVKVTGEPK PEITWWFEGE ILQDGEDYQY IERGETYCLY LPETFPEDGG EYMCKAVNNK GSAASTCILT IESKN
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產(chǎn)品描述：

人Nexilin（Nexn）是一種參與肌肉收縮的蛋白質(zhì)，在肌肉細(xì)胞的運(yùn)動過程中發(fā)揮著重要的作用。如果想要重組這種蛋白，可以按照以下步驟進(jìn)行：
克隆Nexn基因：可以使用PCR技術(shù)從人的基因組DNA中擴(kuò)增Nexn基因的開放閱讀框（ORF）序列。此外，還需要添加一些啟動子和標(biāo)簽序列，以便在表達(dá)過程中調(diào)節(jié)基因表達(dá)，并且可以方便地檢測到蛋白質(zhì)。

構(gòu)建表達(dá)載體：將Nexn基因插入表達(dá)載體中，可以選擇一些經(jīng)過驗(yàn)證的表達(dá)載體，如pET系列載體。這些載體中包含了一些促進(jìn)蛋白表達(dá)的元件，如啟動子、增強(qiáng)子、終止子等。

選擇表達(dá)宿主細(xì)胞：可以選擇一些常用的表達(dá)宿主細(xì)胞，如哺乳動物細(xì)胞，例如CHO細(xì)胞、HEK293細(xì)胞等，并在細(xì)胞中轉(zhuǎn)染含有Nexn基因的表達(dá)載體。這些宿主細(xì)胞中還需要添加一些輔助蛋白質(zhì)，如轉(zhuǎn)染脂質(zhì)、抗生素等，以幫助蛋白質(zhì)的正確折疊和純化。

蛋白表達(dá)和純化：在表達(dá)宿主細(xì)胞中誘導(dǎo)Nexn蛋白的表達(dá)，并通過細(xì)胞破碎和離心等方法提取蛋白質(zhì)。然后使用親和層析、離子交換層析、凝膠過濾層析等方法對蛋白進(jìn)行純化。

蛋白質(zhì)的鑒定和活性檢測：使用SDS-PAGE、Western blot等方法對純化后的Nexn蛋白進(jìn)行鑒定和定量。此外，還可以使用細(xì)胞實(shí)驗(yàn)等方法對Nexn蛋白的生物活性進(jìn)行檢測，如檢測其對肌肉細(xì)胞的收縮和運(yùn)動的影響。
Datasheet ：

Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

功能：

Involved in regulating cell migration through association with the actin cytoskeleton. Has an essential role in the maintenance of Z line and sarcomere integrity.
基因功能參考文獻(xiàn)：
1. NELINinduced phenotypic transformation of human vascular smooth muscle cell was regulated via the RhoA/SRF signaling pathway. PMID: 26458985
2. NEXN as a novel gene for ASD and its function to inhibit GATA4 established a critical regulation of an F-actin binding protein on a transcription factor in cardiac development PMID: 24866383
3. This is the first study to identify NEXN as a novel coronary artery disease susceptibility gene with both genetic and functional evidence. PMID: 24349201
4. Nexilin is a component of the machinery that drives the formation of Listeria monocytogenes comet tails and enteropathogenic Escherichia coli pedestals. PMID: 22381134
5. the mutations in NEXN that we describe here may further expand the knowledge of Z-disc genes in the pathogenesis of HCM. PMID: 20970104
6. NELIN product is an F-actin associated protein and mediates cell motility PMID: 15823560
7. The findings of thi study on the role of nexilin in maintaining cardiac Z-disk integrity and on the workload dependence to cardiac Z-disk damage in nexilin-linked cardiomyopathy also might have implications for the treatment of NEXN-mutation carriers. PMID: 19881492
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相關(guān)疾病：

Cardiomyopathy, dilated 1CC (CMD1CC); Cardiomyopathy, familial hypertrophic 20 (CMH20)
亞細(xì)胞定位：

Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cytoplasm, myofibril, sarcomere, Z line.
組織特異性：

Abundantly expressed in heart and skeletal muscle, and at lower levels in placenta, lung, liver and pancreas. Also expressed in HeLaS3 and MOLT-4 cell lines.
數(shù)據(jù)庫鏈接：

HGNC: 29557

OMIM: 613121

KEGG: hsa:91624

STRING: 9606.ENSP00000333938

UniGene: Hs.612385