Recombinant Human NEDD8-conjugating enzyme Ubc12 (UBE2M)

中文名稱：

人UBE2M重組蛋白
貨號：

CSB-YP025468HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人UBE2M重組蛋白
貨號：

CSB-EP025468HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人UBE2M重組蛋白
貨號：

CSB-EP025468HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人UBE2M重組蛋白
貨號：

CSB-BP025468HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人UBE2M重組蛋白
貨號：

CSB-MP025468HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

UBE2M
Uniprot No.：

P61081
別名：

hUbc12; NEDD8 carrier protein; NEDD8 conjugating enzyme Ubc12; NEDD8 protein ligase; NEDD8-conjugating enzyme Ubc12; UBC-RS2; UBC12; UBC12_HUMAN; ube2m; ubiquitin carrier protein M; Ubiquitin conjugating enzyme E2 M; Ubiquitin-conjugating enzyme E2 M; ubiquitin-protein ligase M; yeast UBC12 homolog
種屬：

Homo sapiens (Human)
蛋白長度：

Full length protein
表達區域：

1-183
氨基酸序列

MIKLFSLKQQ KKEEESAGGT KGSSKKASAA QLRIQKDINE LNLPKTCDIS FSDPDDLLNF KLVICPDEGF YKSGKFVFSF KVGQGYPHDP PKVKCETMVY HPNIDLEGNV CLNILREDWK PVLTINSIIY GLQYLFLEPN PEDPLNKEAA EVLQNNRRLF EQNVQRSMRG GYIGSTYFER CLK
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
基因功能參考文獻：
1. the DCN1-UBC12 interaction is inhibited by DI-591, a high-affinity, cell-permeable small-molecule inhibitor that binds to purified recombinant human DCN1 and DCN2 proteins with K i values of 10-12 nM PMID: 29074978
2. Furthermore, the authors characterized SENP8/DEN1 as the protease that counteracts Ubc12 auto-neddylation, and observed aberrant neddylation of Ubc12 and other NEDD8 conjugation pathway components in SENP8-deficient cells. PMID: 28475037
3. GlyRS preferentially binds and protects Ubc12. PMID: 27348078
4. Polymorphisms in the UBE2M gene are associated with cetuximab efficacy in colorectal cancer. PMID: 26206335
5. UBE2M is required to maintain genome integrity by activating multiple Cullin ligases throughout the cell cycle. PMID: 25025768
6. Distinct preferences of UBC12 and UBE2F peptides for inhibiting different DCNLs, including the oncogenic DCNL1. PMID: 23201271
7. Our results demonstrate a role for UBE2M in mediating cytotoxicity of gemcitabine in human urothelial carcinoma cells. PMID: 21477582
顯示更多

收起更多
蛋白家族：

Ubiquitin-conjugating enzyme family, UBC12 subfamily
數據庫鏈接：

HGNC: 12491

OMIM: 603173

KEGG: hsa:9040

STRING: 9606.ENSP00000253023

UniGene: Hs.406068