Recombinant Human Mitochondrial cardiolipin hydrolase (PLD6)

中文名稱：

人PLD6重組蛋白
品名簡稱：

Recombinant Human PLD6 protein
貨號：

CSB-EP836649HUe1
說明書：

MSDS Datasheet
規(guī)格：

￥2832
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

PLD6
Uniprot No.：

Q8N2A8
別名：

Choline phosphatase 6; Mitochondrial cardiolipin hydrolase; Mitochondrial phospholipase; MitoPLD; Phosphatidylcholine hydrolyzing phospholipase D6; Phosphatidylcholine-hydrolyzing phospholipase D6; Phospholipase D family member 6; Phospholipase D6; PLD 6; pld6; PLD6_HUMAN; Protein zucchini homolog; ZUC; Zucchini, Drosophila, homolog of
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

28.3 kDa
表達區(qū)域：

1-252aa
氨基酸序列

MGRLSWQVAAAAAVGLALTLEALPWVLRWLRSRRRRPRREALFFPSQVTCTEALLRAPGAELAELPEGCPCGLPHGESALSRLLRALLAARASLDLCLFAFSSPQLGRAVQLLHQRGVRVRVVTDCDYMALNGSQIGLLRKAGIQVRHDQDPGYMHHKFAIVDKRVLITGSLNWTTQAIQNNRENVLITEDDEYVRLFLEEFERIWEQFNPTKYTFFPPKKSHGSCAPPVSRAGGRLLSWHRTCGTSSESQT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

Tag-Free
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產(chǎn)品描述：

重組人線粒體心磷脂水解酶（PLD6）是一種在線粒體中表達的酶，其功能與膜脂代謝和線粒體DNA修復等生物過程有關(guān)。下面是重組人PLD6的一般步驟：
設(shè)計和合成基因：基于人PLD6的氨基酸序列，設(shè)計并合成含有PLD6基因的DNA序列。這可以通過基因合成公司或PCR方法完成。

克隆基因：將PLD6基因插入到表達載體中，這可以通過PCR擴增和限制性內(nèi)切酶切割的方法完成。建議選擇一些經(jīng)過驗證的表達載體，如pET系列載體，這些載體中包含了一些促進蛋白表達的元件，如啟動子、增強子、終止子等。

轉(zhuǎn)染宿主細胞：在細胞中轉(zhuǎn)染含有PLD6基因的表達載體。這些宿主細胞可以選擇常見的哺乳動物細胞系，如CHO細胞、HEK293細胞等。

培養(yǎng)和表達蛋白：根據(jù)表達載體中的啟動子和調(diào)節(jié)元件，誘導PLD6蛋白在轉(zhuǎn)染的宿主細胞中表達。在培養(yǎng)期間，需要添加抗生素等來保持表達載體的穩(wěn)定。

蛋白質(zhì)的純化：通過細胞破碎和離心等方法提取含有PLD6蛋白的細胞裂解液。然后使用親和層析、離子交換層析、凝膠過濾層析等方法對蛋白進行純化。

蛋白質(zhì)的鑒定和活性檢測：使用SDS-PAGE、wb等方法對純化后的PLD6蛋白進行鑒定和定量。此外，可以使用酶活性分析等方法來檢測其生物學活性和功能。

需要注意的是，線粒體蛋白的表達和純化需要特殊的條件和方法。因此，在進行PLD6的表達和純化時，需要注意線粒體蛋白的穩(wěn)定性和功能性，避免蛋白質(zhì)的降解和失活。
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Presents phospholipase and nuclease activities, depending on the different physiological conditions. Interaction with Mitoguardin (MIGA1 or MIGA2) affects the dimer conformation, facilitating the lipase activity over the nuclease activity. Plays a key role in mitochondrial fusion and fission via its phospholipase activity. In its phospholipase role, it uses the mitochondrial lipid cardiolipin as substrate to generate phosphatidate (PA or 1,2-diacyl-sn-glycero-3-phosphate), a second messenger signaling lipid. Production of PA facilitates Mitofusin-mediated fusion, whereas the cleavage of PA by the Lipin family of phosphatases produces diacylgycerol (DAG) which promotes mitochondrial fission. Both Lipin and DAG regulate mitochondrial dynamics and membrane fusion/fission, important processes for adapting mitochondrial metabolism to changes in cell physiology. Mitochondrial fusion enables cells to cope with the increased nucleotide demand during DNA synthesis. Mitochondrial function and dynamics are closely associated with biological processes such as cell growth, proliferation, and differentiation. Mediator of MYC activity, promotes mitochondrial fusion and activates AMPK which in turn inhibits YAP/TAZ, thereby inducing cell growth and proliferation. The endonuclease activity plays a critical role in PIWI-interacting RNA (piRNA) biogenesis during spermatogenesis. Implicated in spermatogenesis and sperm fertility in testicular germ cells, its single strand-specific nuclease activity is critical for the biogenesis/maturation of PIWI-interacting RNA (piRNA). MOV10L1 selectively binds to piRNA precursors and funnels them to the endonuclease that catalyzes the first cleavage step of piRNA processing to generate piRNA intermediate fragments that are subsequently loaded to Piwi proteins. Cleaves either DNA or RNA substrates with similar affinity, producing a 5' phosphate end, in this way it participates in the processing of primary piRNA transcripts. piRNAs provide essential protection against the activity of mobile genetic elements. piRNA-mediated transposon silencing is thus critical for maintaining genome stability, in particular in germline cells when transposons are mobilized as a consequence of wide-spread genomic demethylation. PA may act as signaling molecule in the recognition/transport of the precursor RNAs of primary piRNAs. Interacts with tesmin in testes, suggesting a role in spermatogenesis via association with its interacting partner.
基因功能參考文獻：
1. Propose that MIGA proteins promote mitochondrial fusion by regulating mitochondrial phospholipid metabolism via MitoPLD. PMID: 26711011
2. MitoPLD performs a critical function in a pathway that creates a specialized form of RNAi required by developing spermatocytes to suppress transposon mobilization during meiosis. PMID: 22281006
3. Data suggest that mitochondrial-surface phosphatidic acid generated by MitoPLD/Zuc recruits or activates nuage components critical for piRNA production. PMID: 21397848
4. PLD6, aka MitoPLD, encodes an N-terminal mitochondrial localization sequence, localizes to the outer mitochondrial surface, generates phosphatidic acid, a signaling lipid, and triggers trans-mitochondrial association; involved in mitochondrial fusion. PMID: 17028579
亞細胞定位：

Mitochondrion outer membrane; Single-pass membrane protein. Golgi apparatus.
蛋白家族：

Phospholipase D family, MitoPLD/Zucchini subfamily
組織特異性：

Predominantly expressed in testis and ovary, but not limited to gonads (at protein level). It is also found in brain, heart, pituitary gland, prostate, pancreas, thyroid, bone marrow, lung and muscle.
數(shù)據(jù)庫鏈接：

HGNC: 30447

OMIM: 614960

KEGG: hsa:201164

STRING: 9606.ENSP00000317177

UniGene: Hs.729079