Recombinant Human Endothelin-3 (EDN3)

中文名稱：

人EDN3重組蛋白
品名簡稱：

Recombinant Human EDN3 protein
貨號：

CSB-YP007402HUf3
說明書：

MSDS Datasheet
規格：

￥2208
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

EDN3
Uniprot No.：

P14138
別名：

(ET-3)(Preproendothelin-3)(PPET3)
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
來源：

Yeast
分子量：

7.0 kDa
表達區域：

97-117aa
氨基酸序列

CTCFTYKDKECVYYCHLDIIW
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 6xHis-Myc-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Endothelins are endothelium-derived vasoconstrictor peptides.
基因功能參考文獻：
1. the critical physiological role of the KIT-ET3-NO pathway in fulfilling high demand (exceeding basal level) of endothelium-dependent NO generation for coping with atherosclerosis, pregnancy, and aging, is reported. PMID: 28880927
2. genome-wide association studies in population of women in China: Data suggest that EDN3 (endothelin 3) and EDNRB (endothelin receptor type B) play important roles in the molecular mechanisms underlying cervical cancer. PMID: 27863272
3. EDN3 expression in left internal mammary arteries depends on tissue harvesting technique. PMID: 24647318
4. Down-regulated expression of ET3 attenuates the malignant behaviors of human melanoma cells partially by decreasing the expression of SPARC. PMID: 23904381
5. Waardenburg syndrome type II and mutations of EDNRB, EDN3 and SOX10 genes are responsible for Waardenburg syndrome type IV. (review) PMID: 24379252
6. Our data showed that almost all patients, regardless of individual characteristics such as gender or age, expressed the endothelin receptor genes, but did not express the genes for ET-3. PMID: 23515723
7. These data suggest that autocrine EDN3/EDNRB signaling is essential for maintaining GSCs. Incorporating END3/EDNRB-targeted therapies into conventional cancer treatments may have clinical implication for the prevention of tumor recurrence. PMID: 22013079
8. Finding suggest that mutations in RET and NTRK3 acting together are necessary and sufficient for the appearance of Hirschsprung disease and that the EDN3 mutation acts as a phenotype modifier. PMID: 19556619
9. EDN3 may be considered as a common susceptibility gene for sporadic Hirschsprung disease in a low-penetrance fashion. PMID: 20009762
10. Mutations are found in Hirschsprung's disease in a Chinese population. PMID: 14669347
11. KEL6 red blood cells have endothelin-3-converting enzyme activity PMID: 16423827
12. neither polymorphism nor mutation was observed in EDN3 in Chinese Hirschprung disease patients PMID: 17554617
13. Endothelin-3 molecule is specifically upregulated in metastatic melanoma cells, showing that an abnormal autocrine stimulation pathway involving ET-3 is present in metastatic melanoma cells. PMID: 18346402
14. Endothelin signaling axis activates osteopontin expression through PI3 kinase pathway in A375 melanoma cells. PMID: 18722093
15. ET3 induced activation of IkappaB & MAPK in epithelial cells. ET3 is involved in regulating human colonic epithelial cell proliferation & survival, particularly for goblet cells. PMID: 18832450
16. In conclusion, Multiplex Ligation-dependent Probe Amplification assessment of rearrangements in the RET proto-oncogene and in 3 other associated genes, ZEB2, EDN3 and GDNF did not show any variants in 80 sporadic Hirschsprung disease patients. PMID: 19183406
17. Report on spanish cases of Waardenburg syndrome type 4 with novel mutations in EDN3 and SOX 10 genes. PMID: 19764030
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相關疾病：

Hirschsprung disease 4 (HSCR4); Congenital central hypoventilation syndrome (CCHS); Waardenburg syndrome 4B (WS4B)
亞細胞定位：

Secreted.
蛋白家族：

Endothelin/sarafotoxin family
組織特異性：

Expressed in trophoblasts and placental stem villi vessels, but not in cultured placental smooth muscle cells.
數據庫鏈接：

HGNC: 3178

OMIM: 131242

KEGG: hsa:1908

STRING: 9606.ENSP00000337128

UniGene: Hs.1408