Recombinant Human E3 ubiquitin-protein ligase CHIP (STUB1)

中文名稱：

Recombinant Human E3 ubiquitin-protein ligase CHIP (STUB1)
品名簡稱：

Recombinant Human STUB1 protein
貨號：

CSB-EP892480HU
說明書：

MSDS Datasheet
規(guī)格：

￥1344
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

STUB1
Uniprot No.：

Q9UNE7
別名：

Antigen NY-CO-7;CLL-associated antigen KW-8;Carboxy terminus of Hsp70-interacting protein;RING-type E3 ubiquitin transferase CHIP;STIP1 homology and U box-containing protein 1
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

41.8 kDa
表達(dá)區(qū)域：

1-303aa
氨基酸序列

MKGKEEKEGGARLGAGGGSPEKSPSAQELKEQGNRLFVGRKYPEAAACYGRAITRNPLVAVYYTNRALCYLKMQQHEQALADCRRALELDGQSVKAHFFLGQCQLEMESYDEAIANLQRAYSLAKEQRLNFGDDIPSALRIAKKKRWNSIEERRIHQESELHSYLSRLIAAERERELEECQRNHEGDEDDSHVRAQQACIEAKHDKYMADMDELFSQVDEKRKKRDIPDYLCGKISFELMREPCITPSGITYDRKDIEEHLQRVGHFDPVTRSPLTQEQLIPNLAMKEVIDAFISENGWVEDY
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

C-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Collaborates with ATXN3 in the degradation of misfolded chaperone substrates: ATXN3 restricting the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. Ubiquitinates EPHA2 and may regulate the receptor stability and activity through proteasomal degradation. Acts as a co-chaperone for HSPA1A and HSPA1B chaperone proteins and promotes ubiquitin-mediated protein degradation. Negatively regulates the suppressive function of regulatory T-cells (Treg) during inflammation by mediating the ubiquitination and degradation of FOXP3 in a HSPA1A/B-dependent manner. Likely mediates polyubiquitination and downregulates plasma membrane expression of PD-L1/CD274, an immune inhibitory ligand critical for immune tolerance to self and antitumor immunity. Negatively regulates TGF-beta signaling by modulating the basal level of SMAD3 via ubiquitin-mediated degradation. May regulate myosin assembly in striated muscles together with UBE4B and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation. Mediates ubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation.
基因功能參考文獻(xiàn)：
1. Data suggest that the molecular basis of genetic defects in CHIP that cause SCAR16 is that most mutations observed in patients with SCAR16 result in destabilization of CHIP leading to down-regulation of it's biquitination function. (SCAR16 = spinocerebellar ataxia autosomal recessive type 16) PMID: 29317501
2. these findings suggest that CHIP plays a role in negative regulation of PINK1 stability and may suppress PINK1's cytoprotective effect during staurosporine-induced mammalian cell death. PMID: 29242192
3. Results identified a new role of CHIP in adipocyte differentiation. CHIP interacts with and mediates the ubiquitylation of PPARgamma , which results in negative effects on adipogenesis. PMID: 28059128
4. The overexpression of CHIP significantly increased the migration and invasion of the DU145 cells, which is possible due to activation of the AKT signaling pathway and upregulation of vimentin. The expression level of CHIP was observed to be increased in human prostate cancer tissues compared with the adjacent normal tissue. PMID: 29693147
5. Here the authors show that the mammalian ubiquitin ligase C-terminal Hsp70-interacting protein (CHIP), if freed from chaperones during acute stress, can dock on cellular membranes thus performing a proteostasis sensor function. PMID: 29091030
6. These results suggest that in the early response to stressful stimuli, MLK4beta-MLK3 binding is important for regulating MLK3 activity and MAPK signalling, and after prolonged periods of stress exposure, MLK4beta and MLK3 proteins decline via CHIP-dependent degradation. PMID: 28757353
7. Prostate cancer cells expressing an S273A mutant of CHIP have attenuated AR degradation upon 2-ME treatment compared with cells expressing wild-type CHIP, supporting the idea that CHIP phosphorylation by Aurora A activates its E3 ligase activity for the AR PMID: 28536143
8. CHIP role in lung cancer radioresistance.p21 is a bona fide ubiquitylation substrate for CHIP. PMID: 28232384
9. PC-1 works in conjunction with E3 ligase CHIP to regulate androgen receptor stability and activity. PMID: 27835608
10. Some STUB1 mutations known to cause spinocerebellar ataxia, autosomal recessive 16 have a profound impact on the protein structure, stability, and ability of CHIP to dimerize in vitro. PMID: 28396517
11. These findings provide clinical and imaging support for the notion that CHIP is a crucial converging point of manifold neurodegenerative processes, corresponding with its universal biological function in neurodegeneration and reveal the second STUB1 family with ataxia plus hypogonadism. PMID: 28193273
12. the CHIP/CLEC-2 axis may play an important role in the modulation of immune response. PMID: 27443248
13. The overexpression of CHIP is a potent prognostic factor of a good prognosis in ER-positive breast cancer patients in the postmenopausal phase. PMID: 27334118
14. these findings indicate that the stability of the DDIAS protein is regulated by CHIP/HSP70-mediated proteasomal degradation and that CHIP overexpression stimulates the apoptosis of lung cancer cells in response to DNA-damaging agents PMID: 28079882
15. Study reveals a mechanism that the Warburg effect is regulated by CHIP through its function as an E3 ligase, which mediates the degradation of PKM2 during tumor progression. PMID: 28346425
16. The E3 ubiquitin ligase STUB1 is a negative regulator of both RUNX1 and RUNX1-RUNX1T1. Activation of STUB1 could be a promising therapeutic strategy for RUNX1-RUNX1T1 leukemia. PMID: 28536267
17. Low CHIP expression is associated with metastasis of glioblastoma. PMID: 27546621
18. Data show that carboxyl-terminus of Hsp70-interacting protein (CHIP) promotes polyubiquitination of transglutaminase 2 (TG2) and its subsequent proteasomal degradation. PMID: 26568304
19. Consistent with reduced transcription factor EB (TFEB) activity, accumulation of phosphorylated TFEB in STUB1-deficient cells resulted in reduced autophagy and reduced mitochondrial biogenesis. These studies reveal that the ubiquitin-proteasome pathway participates in regulating autophagy and lysosomal functions by regulating the activity of TFEB. PMID: 28754656
20. we report the identification of an unconventional p14ARF degradation pathway induced by the chaperone HSP90 in association with the E3 ubiquitin ligase C-terminus of HSP70-interacting protein (CHIP). PMID: 27793846
21. C terminus of Hsc70-interacting protein (CHIP) selectively interacted with epidermal growth factor receptor (EGFR) mutants and simultaneously induced their ubiquitination and proteasomal degradation. PMID: 27475501
22. Study reveals an important function of CHIP-mediated proteolysis in insulin and IGF1 signaling; upon proteotoxic stress conditions and during aging, CHIP is recruited toward disposal of misfolded proteins, reducing its capacity to degrade the INSR; identify a degradation pathway that controls the level of active DAF-2/INSR in C. elegans, Drosophila and human cells. PMID: 28431247
23. Overexpression of CHIP decreased intracellular protein levels of both G2385R mutant and wild-type LRRK2, while short interfering RNA CHIP knockdown had the opposite effect PMID: 28320779
24. CHIP directly regulates the stability of CD166 protein through the ubiquitin proteasome system. PMID: 28279658
25. Data show that BAG2 Inhibits CHIP-Mediated HSP72 ubiquitination in aged cells. PMID: 28042827
26. Data show that transcription factor regulatory factor X 1 (RFX1) protein expression can be tightly regulated by polyubiquitination-mediated proteosomal degradation via STIP1 homology and U-box containing protein 1 (STUB1). PMID: 27283392
27. CHIP may serve as a promising prognostic biomarker for non-small cell lung cancer (NSCLC] patients and it may be involved in NSCLC angiogenesis through regulating VEGF secretion and expression of VEGFR2. PMID: 27392029
28. Cdk5-mediated phosphorylation of CHIP negatively regulates its neuroprotective function, thereby contributing to neuronal cell death progression following neurotoxic stimuli. PMID: 26206088
29. CHIP is a bona fide negative regulator of the RIPK1-RIPK3 necrosome formation leading to desensitization of TNF-mediated necroptosis PMID: 26900751
30. Protein-protein interactions modulate the docking-dependent E3-ubiquitin ligase activity of CHIP. PMID: 26330542
31. CHIP is required for protein quality control (PQC) and CHIP knockdown diminished cellular PQC capacity in lens cells. PMID: 26321754
32. Data show that the E3 ubiquitin ligase CHIP interacts with protein arginine methyltransferase-5 (PRMT5) both in vivo and in vitro. PMID: 26658161
33. These results indicate that CHIP decreases the Kv1.5 protein level and functional channel by facilitating its degradation in concert with chaperone Hsc70 PMID: 26232501
34. Data suggest that CHIP plays roles in the regulation of autophagic flux. PMID: 26219223
35. detailed and systematic investigation to characterize if there are significant differences in the CHIP in vitro ubiquitination of human Hsp70 and Hsc70. PMID: 26010904
36. CHIP stabilizes amyloid precursor protein via proteasomal degradation and p53-mediated trans-repression of BACE1. PMID: 25773675
37. Our findings demonstrate for the first time that CHIP may be involved in RCC angiogenesis through regulating VEGF secretion and expression of VEGFR2. PMID: 26021863
38. High expression of CHIP is associated with HBV-related Hepatocellular Carcinoma. PMID: 25987026
39. results indicate that the post-endocytic ubiquitination of CFTR by CHIP is a critical step in the peripheral quality control of cell surface DeltaF508 CFTR PMID: 25879443
40. these observations provide functional evidence for CHIP behaviors as a tumor suppressor in gastric cancer. PMID: 25672477
41. CHIP docks onto Hsp70/Hsc70 and defines a bipartite mode of interaction between TPR domains and their binding partners. PMID: 25684577
42. CHIP masks genetic variations to suppress heterogeneous Bcl-2 expression levels and prevents augmentation of the anticancer drug-resistant population of breast cancer cells. PMID: 25435366
43. miR-1178 acts as an oncomiR in pancreatic cancer cells by inhibiting CHIP expression. PMID: 25635996
44. CHIP/TRAF3/NIK interactions recruit NIK to E3 ligase complexes for ubiquitination and degradation, thus maintaining NIK at low levels PMID: 25792747
45. Whether the clinical heterogeneity seen in STUB1 ARCA can be related to the location of the mutations remains to be understood PMID: 25258038
46. These observations indicate that CHIP serves as a novel tumor suppressor by downregulating EGFR pathway in pancreatic cancer cells, decreased expression of CHIP was associated with poor prognosis in pancreatic cancer. PMID: 24722501
47. CHIP-mediated ubiquitination of IRE1 contributes to the dynamic regulation of the unfolded protein response. PMID: 25225294
48. From our results, we propose that inhibition of CSC properties may be one of the functions of CHIP as a suppressor of cancer progression. PMID: 25234599
49. CHIP interacted with eIF5A and mediated its ubiquitination for degradation. PMID: 24509416
50. Ebp1 p42 isoform regulates the proteasomal degradation of the p85 regulatory subunit of PI3K by recruiting a chaperone-E3 ligase complex HSP70/CHIP. PMID: 24651434
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相關(guān)疾病：

Spinocerebellar ataxia, autosomal recessive, 16 (SCAR16)
亞細(xì)胞定位：

Cytoplasm. Nucleus.
組織特異性：

Expressed in differentiated myotubes (at protein level). Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung.
數(shù)據(jù)庫鏈接：

HGNC: 11427

OMIM: 607207

KEGG: hsa:10273

STRING: 9606.ENSP00000219548

UniGene: Hs.592081