Recombinant Human E3 ubiquitin-protein ligase BRE1B (RNF40), partial

中文名稱：

人RNF40重組蛋白
貨號：

CSB-YP019890HU
規格：
來源：

Yeast
其他：

中文名稱：

人RNF40重組蛋白
貨號：

CSB-EP019890HU
規格：
來源：

E.coli
其他：

中文名稱：

人RNF40重組蛋白
貨號：

CSB-EP019890HU-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人RNF40重組蛋白
貨號：

CSB-BP019890HU
規格：
來源：

Baculovirus
其他：

中文名稱：

人RNF40重組蛋白
貨號：

CSB-MP019890HU
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

RNF40
Uniprot No.：

O75150
別名：

95 kDa retinoblastoma associated protein; 95 kDa retinoblastoma protein binding protein; 95 kDa retinoblastoma-associated protein; BRE 1B; BRE1 B; BRE1 E3 ubiquitin ligase homolog B; BRE1-B; BRE1B; BRE1B_HUMAN; DKFZp686K191; E3 ubiquitin protein ligase BRE1B; E3 ubiquitin-protein ligase BRE1B; KIAA0661; MGC13051; Rb associated protein; RBP 95; RBP95; RING finger protein 40; Ring finger protein 40 E3 ubiquitin protein ligase; RNF 40; Rnf40; STARING
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes.
基因功能參考文獻：
1. the RNF20/40 complex, a major ubiquitin ligase catalysing histone H2B monoubiquitination, interacts with the motor protein Eg5 during mitosis and participates in spindle assembly. PMID: 27557628
2. The authors also show that the RING domains of RNF20 and RNF40 can form a stable heterodimer that is active. PMID: 27569044
3. Manipulation of key H2Bub1 E3 ubiquitin ligases, RNF20, RNF40 and BRCA1, in ovarian cancer cell line models modulated H2Bub1 levels, indicative of the role of these RING finger ligases in monoubiquitination of H2Bub1 in vitro PMID: 27798111
4. We show that Bre1 (human BRE1A/B (RNF20/40) and mouse Bre1a/b (Rnf20/40)) acts as an important suppressor of chromosomal instability PMID: 22354749
5. our results suggest that RNF20 and RNF40, either via ubiquitylation of H2B or other targets, are coupled to the proliferation of prostate cancer cells. PMID: 22155569
6. RNF40 cooperates with SUPT16H to induce dynamic changes in chromatin structure during DNA double-strand break repair. PMID: 22031019
7. the observed defects in the radiation response of Bre1a/b-deficient cells PMID: 20738173
8. Formation of trimeric complex UbcH6 and RNF20/40 with PAF stimulates histone 2B monoubiquitination activity in vitro PMID: 16307923
9. Functional Analysis of the rat counterpart PMID: 12121982
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亞細胞定位：

Nucleus.
蛋白家族：

BRE1 family
組織特異性：

Ubiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine.
數據庫鏈接：

HGNC: 16867

OMIM: 607700

KEGG: hsa:9810

STRING: 9606.ENSP00000325677

UniGene: Hs.65238