Recombinant Human DNA-directed RNA polymerases I, II, and III subunit RPABC1 (POLR2E)

中文名稱：

人POLR2E重組蛋白
貨號：

CSB-YP018332HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

人POLR2E重組蛋白
貨號：

CSB-EP018332HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

人POLR2E重組蛋白
貨號：

CSB-EP018332HU-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人POLR2E重組蛋白
貨號：

CSB-BP018332HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

人POLR2E重組蛋白
貨號：

CSB-MP018332HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
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靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

POLR2E
Uniprot No.：

P19388
別名：

and III subunit ABC1; and III subunit RPABC1; DNA directed RNA polymerase II 23 kda polypeptide; DNA directed RNA polymerase II 23 kDa polypeptide; DNA directed RNA polymerase II polypeptide E; DNA directed RNA polymerase II subunit E; DNA directed RNA polymerases I II and III subunit RPABC1; DNA-directed RNA polymerase II 23 kDa polypeptide; DNA-directed RNA polymerase II subunit E; DNA-directed RNA polymerases I; hRPB25; hsRPB5; II; Polr2e; Polymerase (RNA) II (DNA directed) polypeptide E (25kD); Polymerase (RNA) II (DNA directed) polypeptide E, 25kDa; RNA polymerases I; RNA polymerases I, II, and III subunit ABC1; RPAB1_HUMAN; RPABC1; RPB5; RPB5 homolog; XAP4
種屬：

Homo sapiens (Human)
蛋白長度：

Full length protein
表達區(qū)域：

1-210
氨基酸序列

MDDEEETYRL WKIRKTIMQL CHDRGYLVTQ DELDQTLEEF KAQSGDKPSE GRPRRTDLTV LVAHNDDPTD QMFVFFPEEP KVGIKTIKVY CQRMQEENIT RALIVVQQGM TPSAKQSLVD MAPKYILEQF LQQELLINIT EHELVPEHVV MTKEEVTELL ARYKLRENQL PRIQAGDPVA RYFGIKRGQV VKIIRPSETA GRYITYRLVQ
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process.
基因功能參考文獻：
1. POLR2E rs3787016T allele was associated with an increased cancer risk in Chinese population PMID: 29724531
2. POLR2E rs3787016 C/T and HULC rs7763881 were associated with a significantly decreased risk for ESCC 0.005). ANRIL rs2151280 T/C SNP was not associated with risk of ESCC. PMID: 25874495
3. ELL has an early and essential role during rapid high-amplitude gene expression that is required for both Pol II pause site entry and release PMID: 22252557
4. crystal of RPB5 diffracted to 2.8 A resolution and belonged to space group P4(3)2(1)2. PMID: 22102239
5. RPB5-mediating protein is required for the proliferation of hepatocellular carcinoma cells. PMID: 21310960
6. Results pinpoint critical residues for RNA polymerase II subunit 5 binding to RAP30 and/or to HBx, and identify these residues in both mammalian cells and in an in vitro binding assay. PMID: 16169872
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亞細胞定位：

Nucleus.
蛋白家族：

Archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family
數(shù)據(jù)庫鏈接：

HGNC: 9192

OMIM: 180664

KEGG: hsa:5434

STRING: 9606.ENSP00000215587

UniGene: Hs.24301