Recombinant Human Cysteine protease ATG4D (ATG4D)

中文名稱：

Recombinant Human Cysteine protease ATG4D(ATG4D)
貨號：

CSB-YP774797HU
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Human Cysteine protease ATG4D(ATG4D)
貨號：

CSB-EP774797HU
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Human Cysteine protease ATG4D(ATG4D)
貨號：

CSB-EP774797HU-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Human Cysteine protease ATG4D(ATG4D)
貨號：

CSB-BP774797HU
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Human Cysteine protease ATG4D(ATG4D)
貨號：

CSB-MP774797HU
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

ATG4D
Uniprot No.：

Q86TL0
別名：

9830134P12Rik; APG4 autophagy 4 homolog D; APG4 D; APG4; S. cerevisiae; homolog of; D; APG4D; ATG4 autophagy related 4 homolog D (S. cerevisiae); Atg4d; ATG4D_HUMAN; Atg4dl; AUT like 4 cysteine endopeptidase; AUT-like 4 cysteine endopeptidase; AUTL4; Autophagin 4; Autophagin-4; autophagy 4; S. cerevisiae; homolog of; D; autophagy related 4D; cysteine peptidase; Autophagy related cysteine endopeptidase 4; Autophagy related protein 4 homolog D; Autophagy-related cysteine endopeptidase 4; Autophagy-related protein 4 homolog D; Cysteine protease ATG4D; Cysteine protease involved in autophagy; MGC31226
種屬：

Homo sapiens (Human)
蛋白長度：

full length protein
表達區域：

1-474
氨基酸序列

MNSVSPAAAQ YRSSSPEDAR RRPEARRPRG PRGPDPNGLG PSGASGPALG SPGAGPSEPD EVDKFKAKFL TAWNNVKYGW VVKSRTSFSK ISSIHLCGRR YRFEGEGDIQ RFQRDFVSRL WLTYRRDFPP LPGGCLTSDC GWGCMLRSGQ MMLAQGLLLH FLPRDWTWAE GMGLGPPELS GSASPSRYHG PARWMPPRWA QGAPELEQER RHRQIVSWFA DHPRAPFGLH RLVELGQSSG KKAGDWYGPS LVAHILRKAV ESCSDVTRLV VYVSQDCTVY KADVARLVAR PDPTAEWKSV VILVPVRLGG ETLNPVYVPC VKELLRCELC LGIMGGKPRH SLYFIGYQDD FLLYLDPHYC QPTVDVSQAD FPLESFHCTS PRKMAFAKMD PSCTVGFYAG DRKEFETLCS ELTRVLSSSS ATERYPMFTL AEGHAQDHSL DDLCSQLAQP TLRLPRTGRL LRAKRPSSED FVFL
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.; Plays a role as an autophagy regulator that links mitochondrial dysfunction with apoptosis. The mitochondrial import of ATG4D during cellular stress and differentiation may play important roles in the regulation of mitochondrial physiology, ROS, mitophagy and cell viability.
基因功能參考文獻：
1. study elucidated a novel Malat1-miR-101-STMN1/RAB5A/ATG4D regulatory network that Malat1 activates autophagy and promotes cell proliferation by sponging miR-101 and upregulating STMN1, RAB5A and ATG4D expression in glioma cells PMID: 28834690
2. The actions of ATG4 family members (particularly ATG4B) are required for the control of autophagosome fusion with late, degradative compartments in differentiating human erythroblasts. PMID: 23508006
3. the import of Atg4D during cellular stress and differentiation may play important roles in the regulation of mitochondrial physiology, reactive oxygen species, mitophagy and cell viability. PMID: 22441018
4. Caspase cleavage of Atg4D stimulates GABARAP-L1 processing and triggers mitochondrial targeting and apoptosis. PMID: 19549685
亞細胞定位：

[Cysteine protease ATG4D]: Cytoplasm.; [Cysteine protease ATG4D, mitochondrial]: Cytoplasm. Mitochondrion matrix.
蛋白家族：

Peptidase C54 family
數據庫鏈接：

HGNC: 20789

OMIM: 611340

KEGG: hsa:84971

STRING: 9606.ENSP00000311318

UniGene: Hs.512799