Recombinant Human Cyclic AMP-dependent transcription factor ATF-2 (ATF2)

中文名稱：

Recombinant Human Cyclic AMP-dependent transcription factor ATF-2(ATF2)
貨號：

CSB-EP002270HU
說明書：

MSDS Datasheet
規格：

￥1344
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

ATF2
Uniprot No.：

P15336
別名：

Activating transcription factor 2; Activating transcription factor 2 splice variant ATF2 var2; ATF 2; Atf-2; Atf2; ATF2 protein; ATF2_HUMAN; cAMP Response Element Binding Protein 2; cAMP response element binding protein CRE BP1; cAMP response element-binding protein CRE-BP1; cAMP responsive element binding protein 2; formerly; cAMP-dependent transcription factor ATF-2; cAMP-responsive element-binding protein 2; CRE BP1; CRE-BP; CREB 2; CREB-2; CREB2; CREBP1; Cyclic AMP dependent transcription factor ATF 2; Cyclic AMP-dependent transcription factor ATF-2; Cyclic AMP-responsive element-binding protein 2; D130078H02Rik; D18875; HB 16; HB16; Histone acetyltransferase ATF2; MGC105211; MGC105222; MGC111558; MGC142504; mXBP; MXBP protein; Tg(Gzma-Klra1)7Wum; TREB 7; TREB7
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

70.5kDa
表達區域：

1-505aa
氨基酸序列

MKFKLHVNSARQYKDLWNMSDDKPFLCTAPGCGQRFTNEDHLAVHKHKHEMTLKFGPARNDSVIVADQTPTPTRFLKNCEEVGLFNELASPFENEFKKASEDDIKKMPLDLSPLATPIIRSKIEEPSVVETTHQDSPLPHPESTTSDEKEVPLAQTAQPTSAIVRPASLQVPNVLLTSSDSSVIIQQAVPSPTSSTVITQAPSSNRPIVPVPGPFPLLLHLPNGQTMPVAIPASITSSNVHVPAAVPLVRPVTMVPSVPGIPGPSSPQPVQSEAKMRLKAALTQQHPPVTNGDTVKGHGSGLVRTQSEESRPQSLQQPATSTTETPASPAHTTPQTQSTSGRRRRAANEDPDEKRRKFLERNRAAASRCRQKRKVWVQSLEKKAEDLSSLNGQLQSEVTLLRNEVAQLKQLLLAHKDCPVTAMQKKSGYHTADKDDSSEDISVPSSPHTEAIQHSSVSTSNGVSSTSKAEAVATSVLTQMADQSTEPALSQIVMAPSSQSQPSGS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-SUMO-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

A cDNA sequence coding for full-length human cyclic AMP-dependent transcription factor ATF2 was inserted a 6xHis-SUMO-tag at the N-terminus and then expressed in E.coli. The resulting protein consists of 1-505aa of human ATF2 and has a calculated molecular weight of 70.5 kDa. Its purity is over 90% determined by SDS-PAGE. Under reducing conditions, the gel presented a molecular mass band of about 73 kDa. Glycosylation contributes to the slightly higher molecular mass. This recombinant ATF2 protein may be used for specific antibody production or in the studies of ATF2-related transcription.

Activating transcription factor 2 (ATF2) is a member of the activating protein-1 (AP1) transcription factor family that regulates gene expression through homo- or hetero-dimerization with other AP1 transcription factors. ATF2 is also a vital mediator of mammalian cell responses to various stimuli, including stress. Alteration of ATF2 expression and localization has also been associated with the pathology, progression, and chemoresistance of extramammary Paget's disease, as well as in prostate and head and neck squamous cancers.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro. In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.
基因功能參考文獻：
1. Our study found that miR-451 regulates the drug resistance of renal cell carcinoma by targeting ATF-2 PMID: 28429654
2. deregulation of the miR-144-5p/ATF2 axis plays an important role in non-small-cell lung cancer cell radiosensitivity. PMID: 29850528
3. p38alpha and ATF2 expression play a crucial role in the malignant phenotypes of ovarian tumor cells and are a markers of poor prognosis in patients with ovarian serous adenocarcinomas. PMID: 28916425
4. activation of JNK was found to be dependent on muscarinic acid receptor induced Ca(2+)/CAMKII as well as ROS. JNK dependent phosphorylation of ATF2/c-Jun transcription factors resulted in TGF-beta transcription and its signaling. PMID: 27708346
5. ATF2 regulated by miR-204 might also play an important role in the regulation of malignant behavior of glioblastoma. PMID: 27588402
6. We further demonstrated the suppressive function of lncRNA#32 in hepatitis B virus and hepatitis C virus infection. lncRNA#32 bound to activating transcription factor 2 (ATF2) and regulated ISG expression. Our results reveal a role for lncRNA#32 in host antiviral responses. PMID: 27582466
7. Results show that ATF2 is highly expressed in renal cell carcinoma (RCC) tissues and promotes RCC cell proliferation, migration and invasion. The study suggests that ATF2 exerts an oncogenic role in RCC. PMID: 27377902
8. These findings point to an oncogenic function for ATF2 in melanoma development that appears to be independent of its transcriptional activity. PMID: 27210757
9. this study demonstrates that CPEB2 alternative splicing is a major regulator of key cellular pathways linked to anoikis resistance and metastasis. PMID: 28904175
10. Noxin facilitated the expression of Cyclin D1 and Cyclin E1 through activating P38-activating transcription factor 2 signaling pathway, thus enhanced cell growth of breast cancer PMID: 28618963
11. these observations suggest that CD99 is involved in the regulation of CD1a transcription and expression by increasing ATF-2. PMID: 27094031
12. This review provides an overview of the currently known upstream regulators and downstream targets of ATF2. [review] PMID: 28212892
13. TNF induces the binding of ATF2 to the TNF-responsive element. PMID: 27821620
14. miR-204 may act as a tumor suppressor by directly targeting ATF2 in non-small cell lung cancer PMID: 26935060
15. the variant alleles of TSG101 rs2292179 and ATF2 rs3845744 were associated with a reduced risk of breast cancer, particularly for subjects with BMI <24 (kg/m(2)) and postmenopausal women, respectively PMID: 26729199
16. Results reveal that mitochondrial ATF2 is associated with the induction of apoptosis and BRAF inhibitor resistance through Bim activation. PMID: 26462148
17. Neisseria meningitidis caused a high level of E-selectin expression elicited by the activity of phosphorylated ATF2 transcription factor on the E-selectin promoter. PMID: 26153406
18. increased expression of the gene encoding PKCepsilon and abundance of phosphorylated, transcriptionally active ATF2 were observed in advanced-stage melanomas and correlated with decreased FUK expression PMID: 26645581
19. CARMA1- and MyD88-dependent activation of Jun/ATF-type AP-1 complexes is a hallmark of ABC diffuse large B-cell lymphomas. PMID: 26747248
20. More terminally differentiated human odontoblasts was ATF-2 positive, as compared to pulpal fibroblasts at various stages of differentiation: ATF-2 is more associated with cell survival rather than cell proliferation. PMID: 25417007
21. Study identified a potential target of miR-451, ATF2, and revealed a novel role of miR-451 in the inhibition of the migratory ability of hepatoma cell lines. PMID: 24968707
22. ATF-2 knockdown blocked VEGF-A-stimulated VCAM-1 expression and endothelial-leukocyte interactions. ATF-2 was also required for other endothelial cell outputs, such as cell migration and tubulogenesis. PMID: 24966171
23. Study demonstrates the role of miR-622 in suppressing glioma invasion and migration mediated by ATF2, and shows that miR-622 expression inversely correlates with ATF2 in glioma patients PMID: 25258251
24. suppression of tumorigenesis by JNK requires ATF2. PMID: 25456131
25. study revealed that, autocrine soluble factors regulate dual but differential role of ATF-2 as a transcription factor or DNA repair protein, which collectively culminate in radioresistance of A549 cells. PMID: 25041846
26. While expression of ATF-2 is not associated with outcome. PMID: 25141981
27. The expression of ATF2 in chondrocytes is involved in apoptosis in Kashin-Beck disease. PMID: 23866832
28. in human HCC tissues, SPTBN1 expression correlated negatively with expression levels of STAT3, ATF3, and CREB2; SMAD3 expression correlated negatively with STAT3 expression PMID: 25096061
29. zymosan-induced il23a mRNA expression is best explained through coordinated kappaB- and ATF2-dependent transcription; and (iii) il23a expression relies on complementary phosphorylation of ATF2 on Thr-69 and Thr-71 dependent on PKC and MAPK activities. PMID: 24982422
30. Data show that salvianolic acid B protects endothelial progenitor cells against oxidative stress by modulating Akt/mTOR/4EBP1, p38 MAPK/ATF2, and ERK1/2 signaling pathways. PMID: 24780446
31. There is synergism between developmental stage-specific recruitments of the ATF2 protein complex and expression of gamma-globin during erythropoiesis. PMID: 24223142
32. An association between ATF2 polymorphisms and heavy alcohol consumption is only weakly supported. PMID: 24338393
33. ATF2 knockdown revealed ATF2-triggered p21(WAF1) protein expression, suggesting p21(WAF1) transactivation through ATF2. PMID: 23800081
34. results therefore suggest that c-MYC induces stress-mediated activation of ATF2 and ATF7 and that these transcription factors regulate apoptosis in response to oncogenic transformation of B cells PMID: 23416976
35. we establish that ATF2 family members physically and functionally interact with TCF1/LEF1 factors to promote target gene expression and hematopoietic tumor cell growth PMID: 23966864
36. Cytoplasmic ATF2 expression was less frequently seen than nuclear expression in malignant mesenchymal tumors. Benign mesenchymal tumors mostly showed much lower nuclear and cytoplasmic ATF2 expression. PMID: 24289970
37. Data indicate that small molecules that block the oncogenic addiction to PKCepsilon signaling by promoting ATF2 nuclear export, resulting in mitochondrial membrane leakage and melanoma cell death. PMID: 23589174
38. Increasing of ATF2 expression is mediated via oxidative stress induced by arsenic in SV-HUC-1 cells, and MAPK pathways are involved. PMID: 23591579
39. these studies show that the IL-1beta-induced increase in intestinal tight junction permeability was regulated by p38 kinase activation of ATF-2 and by ATF-2 regulation of MLCK gene activity PMID: 23656735
40. Phosphorylation of ATF2 by PKCepsilon is the master switch that controls its subcellular localization and function. PMID: 22685333
41. ATF2-Jun heterodimers bind IFNb in both orientations alone and in association with IRF3 and HMGI PMID: 22843696
42. we report the kinetic mechanism for JNK1beta1 with transcription factors ATF2 and c-Jun along with interaction kinetics for these substrates. PMID: 22351776
43. ATF2 subcellular localization is probably modulated by multiple mechanisms PMID: 22275354
44. Data concluded that IR-induced up-regulation of ATF2 was coordinately enhanced by suppression of miR-26b in lung cancer cells, which may enhance the effect of IR in the MAPK signaling pathway. PMID: 21901137
45. The ability of ATF2 to reach the mitochondria is determined by PKCepsilon, which directs ATF2 nuclear localization. Genotoxic stress attenuates PKCepsilon effect on ATF2; enables ATF2 nuclear export and localization at the mitochondria. PMID: 22304920
46. Data show that ATF7-4 is an important cytoplasmic negative regulator of ATF7 and ATF2 transcription factors. PMID: 21858082
47. Our data suggest regulatory roles for ATF2 in TNF-related mechanisms of Head and Neck Squamous Cell Carcinoma. Its perturbation and nuclear activation are associated with significant effects on survival and cytokine production. PMID: 21990224
48. Data suggest that competition between GSTpi and active JNK for the substrate ATF2 may be responsible for the inhibition of JNK catalysis by GSTpi. PMID: 21384452
49. ATF2 interacts with beta-cell-enriched transcription factors, MafA, Pdx1, and beta2, and activates insulin gene transcription. PMID: 21278380
50. MITF is downregulated by ATF2 in the skin of Atf2-/- mice, in primary human melanocytes, and in melanoma cell lines. PMID: 21203491
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亞細胞定位：

Nucleus. Cytoplasm. Mitochondrion outer membrane. Note=Shuttles between the cytoplasm and the nucleus and heterodimerization with JUN is essential for the nuclear localization. Localization to the cytoplasm is observed under conditions of cellular stress and in disease states. Localizes at the mitochondrial outer membrane in response to genotoxic stress. Phosphorylation at Thr-52 is required for its nuclear localization and negatively regulates its mitochondrial localization. Co-localizes with the MRN complex in the IR-induced foci (IRIF).
蛋白家族：

BZIP family, ATF subfamily
組織特異性：

Ubiquitously expressed, with more abundant expression in the brain.
數據庫鏈接：

HGNC: 784

OMIM: 123811

KEGG: hsa:1386

STRING: 9606.ENSP00000264110

UniGene: Hs.592510