Recombinant Human CD81 antigen (CD81), partial
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中文名稱：Recombinant Human CD81 antigen(CD81),partial
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品名簡稱：Recombinant Human CD81 protein, partial
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貨號：CSB-EP004960HU
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說明書：
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規格：￥1344
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圖片：
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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其他：
產品詳情
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純度：Greater than 90% as determined by SDS-PAGE.
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基因名：
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Uniprot No.：
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別名：26 kDa cell surface protein TAPA 1; 26 kDa cell surface protein TAPA-1; 26 kDa cell surface protein TAPA1; CD 81; CD81; CD81 antigen (target of antiproliferative 1); CD81 antigen; CD81 molecule; CD81_HUMAN; CVID6; S5.7; TAPA 1; TAPA1; Target of the antiproliferative 1; Tetraspanin 28; Tetraspanin-28; Tetraspanin28; Tspan 28; Tspan-28; Tspan28
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種屬：Homo sapiens (Human)
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蛋白長度：Extracellular Domain
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來源：E.coli
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分子量：25.8kDa
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表達區域：113-201aa
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氨基酸序列FVNKDQIAKDVKQFYDQALQQAVVDDDANNAKAVVKTFHETLDCCGSSTLTALTTSVLKNNLCPSGSNIISNLFKEDCHQKIDDLFSGK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering. -
蛋白標簽：N-terminal 6xHis-SUMO-tagged
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產品提供形式：Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
緩沖液：If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose. -
儲存條件：Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期：The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期：3-7 business days
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注意事項：Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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產品描述：
The DNA coding sequence translated into the human CD81 protein sequence (113-201aa) was fused with the N-terminal 6xHis-SUMO tag sequence to form the recombinant DNA, which was inserted into an expression vector. The reconstructed expression vector was transformed into the E.coli for follow-up expression. The product underwent purification to obtain the recombinant human CD81 protein with N-terminal 6xHis-SUMO tag. The SDS-PAGE analysis determined its purity higher than 90%. After electrophoresis, a 25 kDa protein band was observed on the gel.
CD81 is a gene providing instructions for making a protein named CD81 antigen (also known as Tspan-28 and CD81) in human and belongs to Tetraspanin (TM4SF) family. CD81 is a tetraspanin molecule mainly expressed on hematolymphoid, neuroectodermal and mesenchymal tumor cell lines.? CD81 forms complexes with CD19, CD21, Leu 13, and integrins on cell membrane and acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Diseases associated with CD81 include immunodeficiency, common Variable and common variable immunodeficiency (CVID6).
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Datasheet & COA：Please contact us to get it.
產品評價
相關產品
靶點詳情
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功能：Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Essential for trafficking and compartmentalization of CD19 receptor on the surface of activated B cells. Upon initial encounter with microbial pathogens, enables the assembly of CD19-CR2/CD21 and B cell receptor (BCR) complexes at signaling TERMs, lowering the threshold dose of antigen required to trigger B cell clonal expansion and antibody production. In T cells, facilitates the localization of CD247/CD3 zeta at antigen-induced synapses with B cells, providing for costimulation and polarization toward T helper type 2 phenotype. Present in MHC class II compartments, may also play a role in antigen presentation. Can act both as positive and negative regulator of homotypic or heterotypic cell-cell fusion processes. Positively regulates sperm-egg fusion and may be involved in acrosome reaction. In myoblasts, associates with CD9 and PTGFRN and inhibits myotube fusion during muscle regeneration. In macrophages, associates with CD9 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. Also prevents the fusion of mononuclear cell progenitors into osteoclasts in charge of bone resorption. May regulate the compartmentalization of enzymatic activities. In T cells, defines the subcellular localization of dNTPase SAMHD1 and permits its degradation by the proteasome, thereby controlling intracellular dNTP levels. Also involved in cell adhesion and motility. Positively regulates integrin-mediated adhesion of macrophages, particularly relevant for the inflammatory response in the lung.; (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes. Association with CLDN1 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell.; (Microbial infection) Involved in SAMHD1-dependent restriction of HIV-1 replication. May support early replication of both R5- and X4-tropic HIV-1 viruses in T cells, likely via proteasome-dependent degradation of SAMHD1.; (Microbial infection) Specifically required for Plasmodium falciparum infectivity of hepatocytes, controlling sporozoite entry into hepatocytes via the parasitophorous vacuole and subsequent parasite differentiation to exoerythrocytic forms.
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基因功能參考文獻：
- CD81 might be a potential prognostic biomarker associated with poor patient prognosis in breast cancer. PMID: 30117494
- Tetraspanin hCD81 backbone domains are critical to signal for productive Hepatitis C Virus entry. A cholesterol-coordinating glutamate residue in CD81 promotes HCV infection. The backbone domains of hCD81 are additional HCV susceptibility-determining factors. PMID: 29677132
- results demonstrate that the interaction of CD81 with SAMHD1 controls the metabolic rate of HIV-1 replication by tuning the availability of building blocks for reverse transcription, namely dNTPs.Together with its role in HIV-1 entry and budding into host cells, the data indicate that HIV-1 uses CD81 as a rheostat that controls different stages of the infection PMID: 28871089
- CD81 is preferentially expressed in first trimester human placentas and progressively down-regulated with gestation advance in normal physiological conditions. CD81 up-regulation is detected in trophoblasts and cells in the villous core, and maternal sera of patients with early-onset severe preeclampsia. PMID: 28167787
- CD81 cell surface expression had a negative impact on survival in acute myeloid leukemia. PMID: 27566555
- Results from crystallography and molecular dynamics of CD81 long-extracellular loop (LEL) show that its flexibility is an inherent molecular property likely to be tuned by variation in pH and redox conditions. This tuning mechanism would explain the priming role ascribed to CD81LEL in rendering the virus-receptor complex fusogenic during cell entry. PMID: 27916518
- Free energy calculations indicated that the E2/CD81 binding process might follow a two-step model involving (i) the electrostatic interaction-driven initial binding of human-specific E2-site2, followed by (ii) changes in the E2 orientation to facilitate the hydrophobic and van der Waals interaction-driven binding of E2-site1 PMID: 28481946
- A new link between HCV receptor molecules and the hepatocyte glycocalyx, namely, CD81 and Synd-1. PMID: 27930836
- Study used molecular dynamics simulations to gain insights into the role of local conformational flexibility in nanodomain formation in the plasma membrane, using the tetraspanin molecule CD81 as a model; suggest that exposing a flexible domain of CD81 enables binding to interaction partners by circumventing the restriction of orientation and conformational freedom of membrane proteins PMID: 27276264
- Studies have shown that CD81 regulates cell migration and invasion, and has therefore been implicated in tumor growth, cancer progression and metastasis. CD81 is expressed in most types of cancer, and the overexpression or down-regulation of this molecule has been correlated with either good or bad prognosis. [review] PMID: 28408492
- The transmembrane segments of CD81 pack as two largely separated pairs of helices, capped by the large extracellular loop (EC2) at the outer membrane leaflet. The two pairs of helices converge at the inner leaflet to create an intramembrane pocket with additional electron density corresponding to a bound cholesterol molecule within the cavity. PMID: 27881302
- Results suggest that the CD81 antigen (CD81) expressed by B cells has differential effects on B cell proliferation or apoptosis according to Epstein-Barr virus (EBV) infection and the expression level of CD81. PMID: 26498453
- CD81 expression was lower in systemic sclerosis patients compared to controls independent of disease duration. PMID: 26926492
- IFI6 inhibits HCV entry by impairing EGFR mediated CD81/CLDN1 interactions. This may be relevant to other virus entry processes employing EGFR. PMID: 25757571
- Data suggest that the intramolecular 188-196 bond restricts the intrinsic conformational dynamics of D-helix of cluster of differentiation 81 (CD81)-large extracellular loop (LEL), which is essential for hepatitis C virus entry. PMID: 26116703
- LDLR was not required for the degradation of CD81 by PCSK9, but its presence strengthened the PCSK9 effect. PMID: 26195630
- These data revealed the crucial role played by His490 and His621 in hepatitis C virus infection, particularly during CD81 binding in cell entry. PMID: 25701820
- findings suggest that homozygous CD81 rs708564 TT may be a genetic modifier for avoiding HCV infection whether as a sole single nucleotide polymorphism or combined with the CLDN1 rs893051 GG genotype PMID: 25934191
- Hsp70/40 stimulated the association of Hsp104 with aggregates and increased the duration of this association PMID: 25635054
- Data indicate that HIV-1 colocalizes with CD81 antigen-lined vesicle compartments in astrocytes. PMID: 24587404
- Vpu-mediated downregulation of CD81 from the surfaces of infected T cells contributed to preserving the infectiousness of viral particles. PMID: 25568205
- The data suggest an important role played by the W(437)LAGLF(442) helix of the hepatitis C virus E2 protein in the hydrophobic interaction with the D-helix of CD81. PMID: 25339761
- We have developed infectious pseudo particles of local 3a-isolate and concluded that a number of liver-specific surface proteins function along with CD81 and SRBI receptor regarding HCV infectivity PMID: 24549717
- Amino acids Y507, V514, and V515 of hepatitis C virus E2 contribute to interaction with HCV receptor CD81. PMID: 24990994
- CLEC4M and CD81 both are still crucial for hepatitis C virus entry into hepatocytes. PMID: 24965233
- Authors show enhanced hepatoma migration and invasion following expression of CD81 and a reduction in invasive potential upon CD81 silencing. PMID: 24662676
- These results strongly suggest that CD81 stimulates melanoma cell motility by inducing MT1-MMP expression through the Akt-dependent Sp1 activation signaling pathway, leading to increased melanoma invasion and metastasis. PMID: 24733393
- study reported on seven CD81 SNP's present in human populations that all facilitate HCV entry in vitro PMID: 24211330
- The large extracellular loop (LEL) of CD81 is a molecule that is highly conserved and it was hypothesised that variation in the CD81 LEL sequence may modify susceptibility to HCV infection; no differences in nucleotide sequence influencing susceptibility to, or outcome of hepatitis C virus infection or evidence of methylation of the gene were found. PMID: 24122777
- Radiation increases the cellular uptake of exosomes through CD29/CD81 complex formation. PMID: 24667602
- results demonstrate important roles of CD81 in both entry and budding stages of the influenza infection cycle. PMID: 24130495
- A specific association between alpha4beta1 and CD81, CD82 and CD151 was demonstrated and antibodies to CD81 and CD82 augmented adhesion of proerythroblasts to Vascular Cell Adhesion Molecule-1. PMID: 23704882
- Authors demonstrated that EWI-2wint promotes CD81 clustering and confinement in CD81-enriched areas. PMID: 23351194
- Two SNPs in the CD81 gene, that encodes the molecule involved in the signal modulation of B lymphocytes, show a strong association with alloimmunization in sickle cell disease. PMID: 23762099
- CD81 interacts with ICAM-1 and CD3 during conjugation between T cells and antigen-presenting cells PMID: 23858057
- HRas signal transduction promotes hepatitis C virus cell entry by triggering the host CD81-CLDN1 complex formation. PMID: 23498955
- Data indicate frequent down-regulation of CD81 expression in gastric cancer cell lines and primary tumor tissues. PMID: 23264205
- In summary, these data highlight the dynamic nature of CD81 and demonstrate a role for CD81 lateral diffusion to regulate hepatitis C virus infection in a polarization-dependent manner. PMID: 23126643
- Interaction of Rac1 with the C-terminal cytoplasmic domain of CD81 is a novel regulatory mechanism of the GTPase activity turnover. PMID: 23264468
- These findings together indicate that the HCV RNA replication status plays a crucial determinant in HCV growth by modulating the expression and intracellular localization of CD81. PMID: 23349980
- CD81 interacts with the T cell receptor to suppress signaling. PMID: 23226274
- Fluorescent Resonance Energy Transfer studies confirm a role for these CD81 residues in claudin-1 association and Hepatitis C virus infection. PMID: 22897233
- data demonstrate that EGFR internalization is critical for hepatitis C virus entry and identify a hitherto-unknown association between CD81 and EGFR PMID: 22855500
- our results show that CD81 may have a relevant role in MM pathogenesis and represent a novel adverse prognostic marker in myeloma. PMID: 22333880
- A novel membrane binding interface was revealed adjacent to the exposed HCV interaction site in the extracellular loop of CD81. PMID: 22740401
- These results suggest that palmitoylation of CD81 should facilitate hepatitis C virus entry, at least in part, by regulating the association of CD81 with tetraspanin-enriched microdomains. PMID: 22560863
- Soluble serum CD81 is elevated in patients with chronic hepatitis C and correlates with alanine aminotransferase serum activity. PMID: 22355327
- Heptatitis c virus (HCV) specific E2 and host CD81 antibodies reduce HCV pseudoparticle entry. PMID: 22074322
- Hepatitis C virus is primed by CD81 protein for low pH-dependent fusion PMID: 21737455
- CD81 is required for the formation of actin membrane protrusions via RAC1 activation in adhesion-dependent immune cell migration. PMID: 21677313
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相關疾?。?/div>Immunodeficiency, common variable, 6 (CVID6)亞細胞定位：Cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein.蛋白家族：Tetraspanin (TM4SF) family組織特異性：Expressed on B cells (at protein level). Expressed in hepatocytes (at protein level). Expressed in monocytes/macrophages (at protein level). Expressed on both naive and memory CD4-positive T cells (at protein level).數據庫鏈接：
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