Recombinant Human ATP-dependent DNA helicase Q5 (RECQL5), partial

^{In
Stock}

中文名稱：

人RECQL5重組蛋白
貨號(hào)：

CSB-EP019539HU
說明書：

MSDS Datasheet
規(guī)格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

RECQL5
Uniprot No.：

O94762
別名：

DNA helicase, RecQ-like type 5;RecQ5;RecQ protein-like 5
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
來源：

E.coli
分子量：

26.1 kDa
表達(dá)區(qū)域：

39-213aa
氨基酸序列

MAVVKGNKDVFVCMPTGAGKSLCYQLPALLAKGITIVVSPLIALIQDQVDHLLTLKVRVSSLNSKLSAQERKELLADLEREKPQTKILYITPEMAASSSFQPTLNSLVSRHLLSYLVVDEAHCVSQWGHDFRPDYLRLGALRSRLGHAPCVALTATATPQVQEDVFAALHLKKPV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

C-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Isoform beta is a DNA helicase that plays an important role in DNA replication, transcription and repair. Inhibits elongation of stalled transcripts at DNA damage sites by binding to the RNA polymerase II subunit POLR2A and blocking the TCEA1 binding site. Required for mitotic chromosome separation after cross-over events and cell cycle progress. Required for efficient DNA repair, including repair of inter-strand cross-links. Stimulates DNA decatenation mediated by TOP2A. Prevents sister chromatid exchange and homologous recombination.
基因功能參考文獻(xiàn)：
1. It has been shown that conjugation of SUMO2, but not SUMO1 or SUMO3, to the essential replication factor PCNA is induced on transcribed chromatin by the RNAPII-bound helicase RECQ5. PMID: 30006506
2. we have shown that increased expression of RECQL5 protein occurs and is likely to contribute to tumourigenesis in UCC and that the pharmacological targeting of the helicase activity of RECQL5 is a strong target for future small molecule inhibitor development. PMID: 27764811
3. RECQ5 removes RAD51 filaments stabilizing stalled replication forks at common fragile sites and hence facilitates CFS cleavage by MUS81-EME1. PMID: 28575661
4. the open and closed forms of RECQL5 were used together with a quantitative comparison of all current RecQ family structures to construct a mechanistic model for RECQL5 helicase activity PMID: 28100692
5. Study characterized the G-quadruplex (GQ) unfolding activity of RECQ5 for different DNA constructs under different salt conditions, showed that RECQ5 is overall a weak GQ destabilizer compared to Bloom and Werner PMID: 27332117
6. interaction between RECQ5 and proliferating cell nuclear antigen (PCNA) promotes RAD18-dependent PCNA ubiquitination and the helicase activity of RECQ5 promotes the processing of replication intermediates. PMID: 27502483
7. RECQL5 has unique strand annealing properties relative to the other human RecQ helicase proteins PMID: 26717024
8. RECQL5 is a critical regulator of genome stability in myeloproliferative neoplasms and demonstrate that replication stress-associated cytotoxicity can be amplified specifically in JAK2V617F mutant cells through RECQL5-targeted synthetic lethality. PMID: 26686625
9. mutation associated with early myocardial infarction PMID: 26844521
10. Expression of RECQL5 in breast cancer can drive proliferation supporting an oncogenic function for RECQL5 in breast cancer. RECQL5 is a promising biomarker in breast cancer. PMID: 26586793
11. RecQ5 prevents transcription associated genome instability by facilitating PIAS1-SRSF1 ligase complex sumoylation of DNA topoisomerase 1. PMID: 25851487
12. results suggested RECQL5 as a tumor suppressor in osteosarcoma and may be a potential therapeutic target for osteosarcoma treatment PMID: 26499077
13. The RECQL5 genetic polymorphism was associated with osteosarcoma in a Chinese population. PMID: 25867335
14. The present study indicated that the RECQL5 genetic polymorphism and haplotypes were associated with breast cancer in a Chinese population. PMID: 25394896
15. RECQL5 as a major determinant for camptothecin resistance in colorectal cancer cells. PMID: 21210765
16. The data presented here provide evidence that RECQL5 plays a general role in the control of transcript elongation in human cells. In its absence, transcript elongation rates increase, the distribution profile of RNAPII is markedly altered across the genome, and higher levels of RNAPII pausing or arrest (i.e., transcription stress) are detected. PMID: 24836610
17. RECQL5 genetic polymorphisms were associated with osteosarcoma in a Chinese population. PMID: 24287950
18. The present study indicated that RECQL5 genetic polymorphism and haplotypes were associated with larynx cancer in a Chinese population. PMID: 24213927
19. The findings suggest that RECQ5 acts during the post-synaptic phase of synthesis-dependent strand annealing to prevent formation of aberrant RAD51 filaments on the extended invading strand, thus limiting its channeling into potentially hazardous crossover pathway of homologous recombination. PMID: 24319145
20. This article reviews the established roles of RECQL5 at the cross roads of DNA replication, recombination and transcription, and propose that human RECQL5 provides important backup functions in the absence of other DNA helicases.[review] PMID: 23627586
21. RECQL5 contacts the Rpb1 jaw domain of Pol II at a site that overlaps with the binding site for the transcription elongation factor TFIIS. Binding of RECQL5 to Pol II interferes with the ability of TFIIS to promote transcriptional read-through in vitro. PMID: 23748380
22. RECQL5 modulates and/or directly participates in base excision repair of endogenous DNA damage. PMID: 22973052
23. The study provides insight into the recruitment and retention dynamics of RECQL5 at DNA double-strand breaks sites and its functional interplay with BLM protein and Werner syndrome protein. PMID: 23180761
24. the recruitment of RECQL5 to laser-induced DSB sites is independent of functional activities of its interacting partners, MRE11 and RNA polymerase II. PMID: 22633600
25. Data indicate taht BRC repeat is a common RAD51 recombinase interaction module that can either promote homologous recombination (HR), as in the case of BRCA2, or to suppress HR, as in RECQL5 helicase. PMID: 22645136
26. study provides novel insights into a mechanism by which RECQ5 regulates the transcription machinery via its dynamic interaction with RNAPII, thereby preventing genome instability PMID: 21402780
27. propose that RECQL5 stabilises the replication fork allowing replication to overcome the effects of thymidine and complete the cell cycle PMID: 20643585
28. Physical interaction of RECQ5 helicase with RAD51 facilitates its anti-recombinase activity. PMID: 20348101
29. Data suggest that RecQL5 promotes genome stabilization by participation in homologous recombination-dependent DNA repair as a RecQ helicase and by regulating the initiation of Pol II to reduce replication impairment and recombination. PMID: 20231364
30. RECQL5beta dramatically stimulates the rate of FEN1 cleavage of flap DNA substrates. PMID: 20081208
31. RECQ5 is a protein with DNA helicase and strand-annealing activities in a single polypeptide. PMID: 15241474
32. RECQ5beta promotes strand exchange between arms of synthetic forked DNA structures resembling a stalled replication fork in a reaction dependent on ATP hydrolysis. PMID: 17003056
33. RECQL5 binds the Rad51 recombinase and inhibits Rad51-mediated D-loop formation. PMID: 18003859
34. The novel intramolecular modulation of RECQ5beta catalytic activity mediated by the zinc-binding motif may represent a universal regulation mode for all RecQ family helicases. PMID: 18290761
35. RecQ5beta has suppressive roles in events associated with RNAP II-dependent transcription PMID: 18419580
36. RECQ5 appears to be the only member of the human RECQ family of helicases that associates with RNAPII. PMID: 18562274
37. RECQL5 inhibits reconstituted, promoter-driven RNAPII transcription in a dose-dependent manner. PMID: 19570979
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亞細(xì)胞定位：

[Isoform Beta]: Nucleus, nucleoplasm.; [Isoform Alpha]: Cytoplasm.; [Isoform Gamma]: Cytoplasm.
蛋白家族：

Helicase family, RecQ subfamily
組織特異性：

Ubiquitous.
數(shù)據(jù)庫鏈接：

HGNC: 9950

OMIM: 603781

KEGG: hsa:9400

STRING: 9606.ENSP00000317636

UniGene: Hs.632229