Recombinant Escherichia coli Ferrichrome-iron receptor (fhuA), partial

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中文名稱：

大腸桿菌fhuA重組蛋白
品名簡(jiǎn)稱：

Recombinant E.coli fhuA protein, partial
貨號(hào)：

CSB-EP362005ENV1c2
說明書：

MSDS Datasheet
規(guī)格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

fhuA
Uniprot No.：

P06971
別名：

Ferric hydroxamate receptor;Ferric hydroxamate uptake;Ferrichrome-iron receptor
種屬：

Escherichia coli (strain K12)
蛋白長(zhǎng)度：

Partial
來源：

E.coli
分子量：

31.7
表達(dá)區(qū)域：

269-387aa
氨基酸序列

QNEPETGYYGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLRFAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 6xHis-Trx-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Involved in the uptake of iron in complex with ferrichrome, a hydroxamate-type siderophore. Binds and transports ferrichrome-iron across the outer membrane. In addition to its role in ferrichrome-iron transport, transports the antibiotic albomycin, which is a structural analog of ferrichrome, and acts as a receptor for colicin M, microcin J25 and bacteriophages T1, T5, phi80 and UC-1. The energy source, which is required for all FhuA functions except infection by phage T5, is provided by the inner membrane TonB system.
基因功能參考文獻(xiàn)：
1. Cor is an outer membrane (OM) lipoprotein that requires the participation of additional outer membrane proteins (OMPs) to exclude FhuA-dependent phages. PMID: 30043202
2. FhuA-PNIPAAm conjugates will serve as functional nanosized building blocks for applications in targeted drug delivery, self-assembly systems, functional membranes and transmembrane protein gated nanoreactors. PMID: 27614163
3. FhuA plug domain was a mostly unfolded structure that was able to occlude isolated FhuA beta-barrels inserted into the cell membrane. PMID: 22846061
4. In vitro experiments establish that FhuA-TonB interactions require sequential steps of kinetically limiting rearrangements. PMID: 15736954
5. during siderophore transport through FhuA, the cork domain remains within the barrel rather than being displaced PMID: 15994322
6. Results identify specific molecular contacts between TonB and outer membrane receptors such as FhuA that extend beyond the well-characterized Ton box. PMID: 16414071
7. the 3.3 angstrom resolution crystal structure of the TonB carboxyl-terminal domain in complex with FhuA PMID: 16741125
8. TonB binding promotes conformational changes in outer surface-exposed loops of FhuA. PMID: 18653801
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亞細(xì)胞定位：

Cell outer membrane; Multi-pass membrane protein.
蛋白家族：

TonB-dependent receptor family
數(shù)據(jù)庫鏈接：

KEGG: ecj:JW0146

STRING: 316385.ECDH10B_0130