Recombinant Escherichia coli Ferric uptake regulation protein (fur)

中文名稱：

大腸桿菌fur重組蛋白
貨號：

CSB-BP364302ENV
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

大腸桿菌fur重組蛋白
貨號：

CSB-EP364302ENV-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大腸桿菌fur重組蛋白
貨號：

CSB-MP364302ENV
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

Name:fur Ordered Locus Names:b0683, JW0669
Uniprot No.：

P0A9A9
別名：

fur; b0683; JW0669Ferric uptake regulation protein; Ferric uptake regulator
種屬：

Escherichia coli (strain K12)
蛋白長度：

Full Length of Mature Protein
表達(dá)區(qū)域：

2-148
氨基酸序列

TDNNTALKK AGLKVTLPRL KILEVLQEPD NHHVSAEDLY KRLIDMGEEI GLATVYRVLN QFDDAGIVTR HNFEGGKSVF ELTQQHHHDH LICLDCGKVI EFSDDSIEAR QREIAAKHGI RLTNHSLYLY GHCAEGDCRE DEHAHEGK
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

功能：

Acts as a global negative controlling element, employing Fe(2+) as a cofactor to bind the operator of the repressed genes. Regulates the expression of several outer-membrane proteins including the iron transport operon.
基因功能參考文獻(xiàn)：
1. The influence of oxygen availability on the levels of active Fur adds a previously undescribed layer of regulation in maintaining cellular iron homeostasis in E. coli. PMID: 29087312
2. High iron supply inhibits the synthesis of the genotoxin colibactin by pathogenic Escherichia coli through a non-canonical Fur/RyhB-mediated pathway. PMID: 28637194
3. Finally, Fur derepression and RecA activation in enterohemorrhagic Escherichia coli O157 were shown to participate in the nitric oxide-enhanced Stx1 and Stx2 production, respectively. PMID: 28294553
4. The authors report that O2 availability affects the gene expression programs of two Escherichia coli master regulators that function in iron homeostasis: the transcription factor Fur and the small RNA regulator RyhB. PMID: 26670385
5. The key regulator of the response to serum-active and inactive-is the iron regulator Fur, which controls the expression of more than 80% of the serum-upregulated genes. PMID: 25118243
6. Fur exhibits a comprehensive regulatory role affecting many fundamental cellular processes linked to iron metabolism in order to coordinate the overall response of E. coli to iron availability. PMID: 25222563
7. Authors demonstrated that the EHEC lpf2 operon is regulated in response to temperature, pH, bile salts and iron, during the exponential phase of growth, and is controlled by Fur. PMID: 24966050
8. In a murine model of urinary tract infection (UTI), deletion of fur alone did not attenuate virulence, whereas a DeltaryhB mutant and a Deltafur DeltaryhB double mutant showed significantly reduced bladder colonization. PMID: 25245805
9. There is a transient change in the activity of the iron regulator Fur and of the oxidative stress regulator SoxS after Zn addition. PMID: 22016571
10. results presented here show that ftnA iron induction is independent of RyhB and instead involves direct interaction of Fe(2+)-Fur with an 'extended' Fur binding site (containing five tandem Fur boxes) located upstream (-83) of the ftnA promoter. PMID: 20015147
11. H2O2-inducible antioxidant defense system leading to an increase in catalase; hydroperoxidase HPI activity, is unaltered in Deltafur mutants PMID: 15878120
12. Tyr 55 of Escherichia coli Fur, as well as the two thymines in positions 18 and 19 of the consensus Fur Box, are involved with binding. PMID: 16212958
13. Affinity of Fur for different metals was determined & ranges over several orders of magnitude in the order: Zn(II)>> Co(II)> Fe(II)> Mn(II). Only Fe(II) binds with sufficient affinity to activate Fur significantly at physiological metal levels. PMID: 16216078
14. an activation mechanism for E. coli Fur involves the folding/unfolding of the N-terminal helix PMID: 16690618
15. Fur protein translation is coupled to that of an upstream open reading frame, translation of which is downregulated by the non-coding RNA RyhB. PMID: 17268550
16. Fur function may be antagonized by intracellular H2O2 by oxidizing the Fur:Fe(2+) complex and inactivating repressor function. PMID: 17462026
17. In this study, information theory was used to identify new Fur binding sites, 13 of which we confirmed experimentally. PMID: 17921503
18. In the fur+ strain compared to those in the fur- strain, expression of chromosomal dihydroorotase activity and beta-galactosidase activity of pyrC-lacZ fusion plasmid was repressed to about 30% and 17%, respectively. PMID: 18176545
19. The characteristic metabolism of rpoS mutants required the activity of Fur (ferric uptake regulator) in order to decrease the synthesis of the small RNA RyhB that might otherwise inhibit the synthesis of iron-rich proteins. PMID: 18556786
20. Binding of both zinc and iron ions induced a similar change in Fur conformations, but shifted residues closer to DNA in a different manner. PMID: 19023664
21. transcriptomic data identified the global transcription regulators ArcA, CRP, Fis, FNR, Fur, BaeR, CpxR, and IHF as targets and potential CO sensors PMID: 19091747
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亞細(xì)胞定位：

Cytoplasm.
蛋白家族：

Fur family
數(shù)據(jù)庫鏈接：

KEGG: ecj:JW0669

STRING: 316385.ECDH10B_0748