Recombinant Escherichia coli Ferric uptake regulation protein (fur)

中文名稱：

大腸桿菌fur重組蛋白
品名簡稱：

Recombinant E.coli fur protein
貨號：

CSB-EP364302ENV
說明書：

MSDS Datasheet
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

fur
Uniprot No.：

P0A9A9
別名：

fur; b0683; JW0669Ferric uptake regulation protein; Ferric uptake regulator
種屬：

Escherichia coli (strain K12)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

20.7kDa
表達區域：

2-148aa
氨基酸序列

TDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLIDMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEFSDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Acts as a global negative controlling element, employing Fe(2+) as a cofactor to bind the operator of the repressed genes. Regulates the expression of several outer-membrane proteins including the iron transport operon.
基因功能參考文獻：
1. The influence of oxygen availability on the levels of active Fur adds a previously undescribed layer of regulation in maintaining cellular iron homeostasis in E. coli. PMID: 29087312
2. High iron supply inhibits the synthesis of the genotoxin colibactin by pathogenic Escherichia coli through a non-canonical Fur/RyhB-mediated pathway. PMID: 28637194
3. Finally, Fur derepression and RecA activation in enterohemorrhagic Escherichia coli O157 were shown to participate in the nitric oxide-enhanced Stx1 and Stx2 production, respectively. PMID: 28294553
4. The authors report that O2 availability affects the gene expression programs of two Escherichia coli master regulators that function in iron homeostasis: the transcription factor Fur and the small RNA regulator RyhB. PMID: 26670385
5. The key regulator of the response to serum-active and inactive-is the iron regulator Fur, which controls the expression of more than 80% of the serum-upregulated genes. PMID: 25118243
6. Fur exhibits a comprehensive regulatory role affecting many fundamental cellular processes linked to iron metabolism in order to coordinate the overall response of E. coli to iron availability. PMID: 25222563
7. Authors demonstrated that the EHEC lpf2 operon is regulated in response to temperature, pH, bile salts and iron, during the exponential phase of growth, and is controlled by Fur. PMID: 24966050
8. In a murine model of urinary tract infection (UTI), deletion of fur alone did not attenuate virulence, whereas a DeltaryhB mutant and a Deltafur DeltaryhB double mutant showed significantly reduced bladder colonization. PMID: 25245805
9. There is a transient change in the activity of the iron regulator Fur and of the oxidative stress regulator SoxS after Zn addition. PMID: 22016571
10. results presented here show that ftnA iron induction is independent of RyhB and instead involves direct interaction of Fe(2+)-Fur with an 'extended' Fur binding site (containing five tandem Fur boxes) located upstream (-83) of the ftnA promoter. PMID: 20015147
11. H2O2-inducible antioxidant defense system leading to an increase in catalase; hydroperoxidase HPI activity, is unaltered in Deltafur mutants PMID: 15878120
12. Tyr 55 of Escherichia coli Fur, as well as the two thymines in positions 18 and 19 of the consensus Fur Box, are involved with binding. PMID: 16212958
13. Affinity of Fur for different metals was determined & ranges over several orders of magnitude in the order: Zn(II)>> Co(II)> Fe(II)> Mn(II). Only Fe(II) binds with sufficient affinity to activate Fur significantly at physiological metal levels. PMID: 16216078
14. an activation mechanism for E. coli Fur involves the folding/unfolding of the N-terminal helix PMID: 16690618
15. Fur protein translation is coupled to that of an upstream open reading frame, translation of which is downregulated by the non-coding RNA RyhB. PMID: 17268550
16. Fur function may be antagonized by intracellular H2O2 by oxidizing the Fur:Fe(2+) complex and inactivating repressor function. PMID: 17462026
17. In this study, information theory was used to identify new Fur binding sites, 13 of which we confirmed experimentally. PMID: 17921503
18. In the fur+ strain compared to those in the fur- strain, expression of chromosomal dihydroorotase activity and beta-galactosidase activity of pyrC-lacZ fusion plasmid was repressed to about 30% and 17%, respectively. PMID: 18176545
19. The characteristic metabolism of rpoS mutants required the activity of Fur (ferric uptake regulator) in order to decrease the synthesis of the small RNA RyhB that might otherwise inhibit the synthesis of iron-rich proteins. PMID: 18556786
20. Binding of both zinc and iron ions induced a similar change in Fur conformations, but shifted residues closer to DNA in a different manner. PMID: 19023664
21. transcriptomic data identified the global transcription regulators ArcA, CRP, Fis, FNR, Fur, BaeR, CpxR, and IHF as targets and potential CO sensors PMID: 19091747
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亞細胞定位：

Cytoplasm.
蛋白家族：

Fur family
數據庫鏈接：

KEGG: ecj:JW0669

STRING: 316385.ECDH10B_0748