Recombinant Escherichia coli 10 kDa chaperonin (groS)

中文名稱：

大腸桿菌groS重組蛋白
貨號：

CSB-YP363855ENV
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

大腸桿菌groS重組蛋白
貨號：

CSB-EP363855ENV
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

大腸桿菌groS重組蛋白
貨號：

CSB-EP363855ENV-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大腸桿菌groS重組蛋白
貨號：

CSB-BP363855ENV
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

大腸桿菌groS重組蛋白
貨號：

CSB-MP363855ENV
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

groS
Uniprot No.：

P0A6F9
別名：

groS; groES; mopB; b4142; JW410210 kDa chaperonin; GroES protein; Protein Cpn10
種屬：

Escherichia coli (strain K12)
蛋白長度：

full length protein
表達區(qū)域：

1-97
氨基酸序列

MNIRPLHDRV IVKRKEVETK SAGGIVLTGS AAAKSTRGEV LAVGNGRILE NGEVKPLDVK VGDIVIFNDG YGVKSEKIDN EEVLIMSESD ILAIVEA
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Together with the chaperonin GroEL, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding, probably by preventing aggregation and by entropically destabilizing folding intermediates. GroES binds to the apical surface of the GroEL ring, thereby capping the opening of the GroEL channel. Regulates the ATPase activity of GroEL.; (Microbial infection) Essential for the assembly of several bacteriophages.
基因功能參考文獻：
1. Single-molecule observation of protein folding in symmetric GroEL-(GroES)2 complexes PMID: 23048033
2. Studies indicate that the amount of GroEL and GroES in an E. coli cell under normal conditions is sufficient to assist in the folding of no more than 5% of the proteins within the cell. PMID: 22177460
3. substrate folding activity of a single ring GroEL variant is stimulated by modulating the cochaperonin GroES PMID: 21757689
4. unfolded GroES has covalent structural changes that lead to amyloid fibril formation detected by NMR PMID: 21507961
5. The association of ATP and GroES to a polypeptide-bound ring of GroEL encapsulates the folding proteins in the central cavity of that ring (cis ring) and allows it to fold in a protected environment where the risk of aggregation is reduced. PMID: 20006619
6. structural analysis of GroEL and its binding to GroES PMID: 15475965
7. the two stages of GroES fibril formation prefer different molecular states of the same protein PMID: 16054644
8. a shift in orientation of GroEL apical domains between the apo-protein and stable GroEL.GroES complexes is highly ordered, and transient conformational intermediates permit the association of GroES before the displacement of bound polypeptide PMID: 16684774
9. GroEL holds the nascent chain on the ribosome in a polypeptide length-dependent manner and post-translationally encapsulates the polypeptide using the GroES cap to accomplish the chaperonin-mediated folding process. PMID: 16754671
10. Coexpression of GroEL and GroES enhanced the growth rate of aconitase producing cells by 30%. PMID: 16822698
11. F170L su substitution has a decreased requirement for the chaperones GroEL and GroES as a result of restoring the temperature-sensitive-folding coat proteins to a wild type-like state. PMID: 17441504
12. Data show that structure-forming characteristics of GroES appear to be conserved through the backbone reversal process, although the differential conformational behavior upon heating also indicates differences. PMID: 18359305
13. The GroEL/GroES compartment is essential for protein folding in vivo. PMID: 18418386
14. there is an an intermediate GroEL/substrate/GroES complex in which the substrate and GroES bind to GroEL by sharing seven common binding sites PMID: 18430731
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亞細胞定位：

Cytoplasm. Note=Exclusively localized in foci, usually near 1 cell pole in mid-to-late exponential phase; polar localization depends on the minCDE operon. Foci form near midcell.
蛋白家族：

GroES chaperonin family
數(shù)據(jù)庫鏈接：

KEGG: ecj:JW4102

STRING: 316385.ECDH10B_4335