Recombinant Drosophila melanogaster Protein groucho (gro), partial

中文名稱：

黑腹果蠅gro重組蛋白
貨號：

CSB-YP322280DLU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

黑腹果蠅gro重組蛋白
貨號：

CSB-EP322280DLU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

黑腹果蠅gro重組蛋白
貨號：

CSB-EP322280DLU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

黑腹果蠅gro重組蛋白
貨號：

CSB-BP322280DLU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

黑腹果蠅gro重組蛋白
貨號：

CSB-MP322280DLU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

gro
Uniprot No.：

P16371
別名：

gro; E(spl)m9/m10; CG8384Protein groucho; Enhancer of split m9/10 protein; E(spl)m9/10
種屬：

Drosophila melanogaster (Fruit fly)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Transcriptional corepressor that regulates transcription when recruited to specific target DNA by hairy-related bHLH proteins. Maternally required for neurogenesis; in the segregation of the neuroectoderm. Directly or indirectly interacts with Notch and Delta.
基因功能參考文獻：
1. Groucho-mediated repression revealed by genome-wide analysis of Groucho binding and activity PMID: 28245789
2. Gro central domains mediate multiple interactions required for repression, thus functioning as a regulatory hub PMID: 26483546
3. Our results demonstrate that Gro is recruited to local sites by transcription factors to attenuate rather than silence gene expression by promoting histone deacetylation and polymerase pausing PMID: 25165826
4. Genes activated by Notch signaling at the dorsal-ventral boundary of wing disc fall into classes affected differently by the loss of kinase module subunits. The distinctions do not result from different requirements for mastermind. PMID: 21793099
5. Gro-mediated histone deacetylation results in increased nucleosome density leading to transcriptional repression. PMID: 20405012
6. Reduction of gro function enhances H mutant phenotypes and suppresses N phenotypes in the adult mechanosensory bristle. This activity of gro is surprising, because it is directed oppositely to its traditionally defined role as a neurogenic gene. PMID: 12154126
7. Comparison of gro and Atro/sbb mutant phenotypes suggests that they do not function together, but instead that they may interact with the same transcription factors. PMID: 16624911
8. Here, we identify several missense mutations in Drosophila Gro, which demonstrate peptide binding to the central pore of the WD (WD40) beta propeller domain in vitro and in vivo PMID: 16762837
9. These results show that homo-oligomerization of Gro is not obligatory for its action in vivo, and that Gro represses transcription through more than one molecular mechanism. PMID: 18034187
10. Phosphorylation of Groucho provides a widespread, long-term mechanism by which receptor tyrosine kinase signals control target gene expression in embryogenesis. PMID: 18216172
11. These findings indicate that Groucho sumoylation increases its corepressor activity by enhancing the recruitment of HDAC1 to Groucho corepressor complex. PMID: 19101520
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亞細胞定位：

Nucleus.
蛋白家族：

WD repeat Groucho/TLE family
數據庫鏈接：

KEGG: dme:Dmel_CG8384

STRING: 7227.FBpp0089115

UniGene: Dm.1340