Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase BRE1-like 1 (HUB1), partial

中文名稱：

擬南芥HUB1重組蛋白
貨號：

CSB-YP823316DOA
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

擬南芥HUB1重組蛋白
貨號：

CSB-EP823316DOA
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

擬南芥HUB1重組蛋白
貨號：

CSB-EP823316DOA-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

擬南芥HUB1重組蛋白
貨號：

CSB-BP823316DOA
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

擬南芥HUB1重組蛋白
貨號：

CSB-MP823316DOA
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

HUB1
Uniprot No.：

Q8RXD6
別名：

HUB1; BRE1A; RDO4; At2g44950/At2g44960; T13E15.24/T13E15.23; T14P1.25/T14P1.24; E3 ubiquitin-protein ligase BRE1-like 1; AtBRE1; EC 2.3.2.27; Protein HISTONE MONOUBIQUITINATION 1; AtHUB1; Protein REDUCED DORMANCY 4; RING-type E3 ubiquitin transferase BRE1-like 1
種屬：

Arabidopsis thaliana (Mouse-ear cress)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

E3 ubiquitin-protein ligase that monoubiquitinates H2B to form H2BK143ub1. H2BK143ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for H3K4me and maybe H3K79me. It thereby plays a central role in histone code and gene regulation. Forms a ubiquitin ligase complex in cooperation with the E2 enzyme UBC2/RAD6. Required for the regulation of flowering time and defense against necrotrophic fungal pathogens. Involved in the control of seed dormancy and germination.
基因功能參考文獻：
1. Two RING E3 ligases, HISTONE MONOUBIQUITINATION 1 and 2 (HUB1 and HUB2), play a role in the selective transcriptional activation of four cuticle biosynthesis genes in Arabidopsis thaliana. PMID: 24319075
2. Data showed that parameters for plant fitness are altered in HUB1 mutant and overexpression lines, suggesting that the histone H2B monoubiquitination status affects plant fitness. PMID: 23073007
3. Double mutants between hub1-1 and elongata (elo) showed an embryo-lethal phenotype, indicating a synergistic genetic interaction. PMID: 22762858
4. role is proposed for chromatin remodeling in seed dormancy by H2B monoubiquitination through HUB1 and HUB2 PMID: 17329563
5. Data show that HUB1 is a functional homolog of the human and yeast BRE1 proteins, adn suggest that it mediates gene activation and cell cycle regulation through chromatin modifications. PMID: 17329565
6. HUB1 upregulates the expression of FLC, and interacts with UBC1 and UBC2 to repress flowering. PMID: 18980658
7. Results describe the role of the Arabidopsis thaliana RING E3 ligase, HISTONE MONOUBIQUITINATION1 (HUB1) in disease resistance. PMID: 19286969
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亞細胞定位：

Nucleus.
蛋白家族：

BRE1 family
組織特異性：

Ubiquitously expressed.
數據庫鏈接：

KEGG: ath:AT2G44950

STRING: 3702.AT2G44950.1

UniGene: At.43266