Phospho-RPS6KB1 (T421+S424) Recombinant Monoclonal Antibody

說明書

中文名稱：

磷酸化-RPS6KB1 (T421+S424)重組抗體
貨號(hào)：

CSB-RA020470A421phHU
規(guī)格：

￥1320
圖片：
Western Blot
Positive WB detected in 293 whole cell lysate(treated with Calyculin A or not)
All lanes Phospho-RPS6KB1 antibody at 0.93μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 70 KDa
Observed band size: 70 KDa

Immunofluorescence staining of Hela cells with CSB-RA020470A421phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunoprecipitating Phospho-RPS6KB1 in Hela whole cell lysate
Lane 1: Rabbit control IgG(1μg)instead of CSB-RA020470A421phHU in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA020470A421phHU(3μg)+ Hela whole cell lysate(1mg)
Lane 3: Hela whole cell lysate (20μg)
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

本品為針對(duì)磷酸化位點(diǎn)T421和S424的重組單克隆抗體，靶向RPS6KB1（核糖體蛋白S6激酶B1），該蛋白是mTOR信號(hào)通路下游關(guān)鍵效應(yīng)分子，通過調(diào)控核糖體生物合成和翻譯起始參與細(xì)胞生長(zhǎng)、增殖及代謝穩(wěn)態(tài)。T421/S424雙位點(diǎn)磷酸化可解除其自抑制結(jié)構(gòu)域作用，促進(jìn)激酶活性激活，在營(yíng)養(yǎng)感應(yīng)、蛋白質(zhì)合成及細(xì)胞周期進(jìn)程中發(fā)揮核心調(diào)控功能。本抗體采用重組單克隆技術(shù)制備，具有高批次一致性和特異性，經(jīng)多種平臺(tái)嚴(yán)格驗(yàn)證：適用于ELISA、Western Blot、免疫熒光（IF）及免疫沉淀（IP）等實(shí)驗(yàn)，推薦工作濃度分別為WB 1:500-1:5000、IF 1:20-1:200、IP 1:200-1:1000。實(shí)驗(yàn)數(shù)據(jù)顯示其對(duì)內(nèi)源性磷酸化RPS6KB1具有優(yōu)異識(shí)別能力，背景信號(hào)低，適用于檢測(cè)細(xì)胞或組織樣本中該蛋白的活化狀態(tài)。該產(chǎn)品為研究mTORC1信號(hào)通路調(diào)控機(jī)制、能量代謝相關(guān)疾病模型及腫瘤微環(huán)境中異常增殖信號(hào)網(wǎng)絡(luò)提供可靠工具，可廣泛應(yīng)用于分子生物學(xué)、細(xì)胞生物學(xué)等基礎(chǔ)研究領(lǐng)域。
Uniprot No.：

P23443
基因名：

RPS6KB1
別名：

70 kDa ribosomal protein S6 kinase 1 antibody; KS6B1_HUMAN antibody; p70 alpha antibody; P70 beta 1 antibody; p70 ribosomal S6 kinase alpha antibody; p70 ribosomal S6 kinase beta 1 antibody; p70 S6 kinase alpha antibody; P70 S6 Kinase antibody; p70 S6 kinase; alpha 1 antibody; p70 S6 kinase; alpha 2 antibody; p70 S6K antibody; p70 S6K-alpha antibody; p70 S6KA antibody; p70(S6K) alpha antibody; p70(S6K)-alpha antibody; p70-alpha antibody; p70-S6K 1 antibody; p70-S6K antibody; P70S6K antibody; P70S6K1 antibody; p70S6Kb antibody; PS6K antibody; Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody; Ribosomal protein S6 kinase beta 1 antibody; Ribosomal protein S6 kinase beta-1 antibody; Ribosomal protein S6 kinase I antibody; RPS6KB1 antibody; S6K antibody; S6K-beta-1 antibody; S6K1 antibody; Serine/threonine kinase 14 alpha antibody; Serine/threonine-protein kinase 14A antibody; STK14A antibody
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from Human Phospho-RPS6KB1 (T421+S424)
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號(hào)：

3B6
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IF, IP

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:20-1:200
IP	1:200-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR. Following activation by mTORC1, phosphorylates EPRS and thereby plays a key role in fatty acid uptake by adipocytes and also most probably in interferon-gamma-induced translation inhibition.
基因功能參考文獻(xiàn)：
1. Expression of miRNAs Targeting mTOR and S6K1 Genes of mTOR Signaling Pathway Including miR-96, miR-557, and miR-3182 in Triple-Negative Breast Cancer PMID: 29862445
2. Studied human ribosomal protein S6 kinase B1 ribosomal protein (p70S6K) expression in pterygium and in normal conjunctival tissues, results show p70S6K activation promotes the transdifferentiation of pterygium fibroblasts to myofibroblasts. PMID: 29270715
3. Akt and p70S6K signaling pathway was highly activated in estrogen receptor-negative (ER-) premalignant breast lesions and ER(-) breast cancer. In addition, p70S6K activation induced transformation of ER(-) human mammary epithelial cells (hMEC). PMID: 28877935
4. ADAR1 contributes to gastric cancer development and progression via activating mTOR/p70S6K/S6 ribosomal protein signaling axis. PMID: 27863387
5. PICT-1 triggers pro-death autophagy through inhibition of rRNA transcription and the inactivation of AKT/mTOR/p70S6K pathway in glioblastoma cells. PMID: 27729611
6. Study found that p70S6K1 plays an important role in gemcitabine chemoresistence. MiR-145 is a tumor suppressor which directly targets p70S6K1 for inhibiting its expression in pancreatic adenocarcinoma. PMID: 27765914
7. These findings suggested that fenofibrate indeed significantly inhibited the proliferation of PC-3cells via apoptotic action, which is associated with the inactivation of the mTOR/p70S6K-dependent cell survival pathway. PMID: 29305864
8. modulation of rDNA transcription initiation, elongation and rRNA processing is an immediate, co-regulated response to altered amino acid abundance, dependent on both mTORC1 activation of S6K1 and MYC activity PMID: 27385002
9. In summary, our data suggested that PYK2 via S6K1 activation modulated AR function and growth properties in prostate cancer cells. Thus, PYK2 and S6K1 may potentially serve as therapeutic targets for PCa treatment. PMID: 27492635
10. Modulation of IL-2, IL-4, IFN-gamma and/or TNF-alpha levels, or inhibitors of Erk1/2 or S6K1 may be a new approach to prevent BAFF-induced aggressive B-cell malignancies. PMID: 27235588
11. Overexpression of AIM2 in hepatocellular carcinoma (HCC) cells suppressed mammalian target of rapamycin (mTOR)-S6K1 pathway and further inhibited proliferation of HCC cells. PMID: 27167192
12. Data show that ribosomal protein S6 kinases, 70-kDa (p70S6K) and interleukin-6 (IL-6) were upregulated in high-metastatic head and neck squamous cell carcinoma (HNSCC) cell lines that underwent epithelial-mesenchymal transition (EMT) when compared to paired low-metastatic cell lines. PMID: 27174914
13. S6K plays a critical role in dopaminergic neuronal differentiation in human neural stem cells. PMID: 26143260
14. Elevated levels of p-Mnk1, p-eIF4E and p-p70S6K proteins are associated with tumor recurrence and poor prognosis in astrocytomas. Overexpression of p-eIF4E and co-expression of p-Mnk1, p-eIF4E and p-p70S6K proteins could be used as novel independent poor prognostic biomarkers for patients with astrocytomas. PMID: 27900644
15. ULK1 has a role in RPS6KB1-NCOR1 repression of NR1H/LXR-mediated Scd1 transcription and augments lipotoxicity in hepatic cells PMID: 27846372
16. function mimicked by the viral protein kinase encoded by open reading frame 36 of Kaposi's sarcoma-associated herpesvirus PMID: 27342859
17. our data suggest that RPS6KB1 is over-activated as p-RPS6KB1 in non-small cell lung cancer, rather than just the total protein overexpressing. The phosphorylation level of RPS6KB1 might be used as a novel prognostic marker for NSCLC patients. PMID: 28792981
18. p54-S6K2 interactome is predominant to the nucleus, whereas p70-S6K1 is predominant to cytosol. PMID: 27493124
19. S6K1 is involved in the regulation of mitochondria morphology and function in HeLa cells. PMID: 27634387
20. S6K1 acts through multiple targets of the mTOR pathway to promote self-renewal and leukemia progression PMID: 27294524
21. S6K1 is a promising tumor-specific target for the enhancement of NSCLC radiosensitivity and its effects may be mediated by increased expression of PDCD4. PMID: 28276898
22. Spheroids showed relative lower activities in the AKT, mammalian target of rapamycin (mTOR) and S6K (also known as RPS6KB1) signaling pathway compared to cells cultured in two dimensions. PMID: 27663511
23. S6K1 phosphorylation of H2B mediates EZH2 trimethylation of H3 early in adipogenesis, contributing to the promotion of obesity. PMID: 27151441
24. Findings indicate that similar to overall cell size growth, Golgi growth is modulated by the "cell growth checkpoint" at late G1 phase through the activities of S6 kinase 1 (S6K1). PMID: 27325676
25. these findings suggest that activation of S6K1 in an adjuvant trastuzumab setting may represent a reliable early tumor marker predicting patient response to trastuzumab, allowing clinicians to further stratify patients for personalized and effective therapy. PMID: 27993682
26. Data indicate YAP1 as a candidate marker to predict cell lines that were most sensitive to MSC2363318A, suggesting clinical development of a dual AKT/P70S6K inhibitor. PMID: 28376174
27. RPS6KB1 single nucleotide polymorphism association with colorectal cancer patients survival PMID: 28138309
28. These data suggest that S6K1-mediated PIPKIgamma90 phosphorylation regulates cell migration and invasion by controlling PIPKIgamma90 degradation. PMID: 27780861
29. Notch3 and pS6 are significantly related to ovarian epithelial cancer development and prognosis, and their combination represents a potential biomarker and therapeutic target in ovarian tumor angiogenesis. PMID: 27445438
30. Taken together, our data provide the first evidence that FXR suppresses proliferation of human liver cancer cells via the inhibition of the mTOR/S6K signaling pathway. FXR expression can be used as a biomarker of personalized mTOR inhibitor treatment assessment for liver cancer patients. PMID: 27109477
31. These results indicated that p-p70S6K may participate in the invasion and metastasis in the development of ESCC and downregulation of the expression of p-p70S6K could improve the sensitivity of cells to rapamycin in ESCC. PMID: 27595116
32. RPS6KB1 SNPs associated with susceptibility to multiple sclerosis in Iranian population. PMID: 28079472
33. We found that S6K1 Iso-2 overexpression in cancer cells promoted cell growth and inhibited apoptosis, denotes its important role on NSCLC survival. PMID: 27460085
34. S6K phosphorylation via the PI3K-PD1 pathway is involved in tau pathology in neurofibrillary tangles and abnormal neurites as well as actin pathology in Hirano bodies. PMID: 26582459
35. These results indicate that the inhibitory effect of rapamycin may be due mainly to increased p14, p15, and p57 expression via promoter demethylation and decreased mTOR and p70S6K expression in ALL cell lines. PMID: 26362858
36. The newly identified miR-195-RPS6KB1 axis partially illustrates the molecular mechanism of prostate cancer progression and represents a novel potential therapeutic target for prostate cancer treatment. PMID: 26080838
37. eIF3 has a role in controlling cell size independently of S6K1-activity PMID: 26172298
38. MiR-497 decreases cisplatin resistance in ovarian cancer cells by targeting mTOR/P70S6K1. PMID: 26238185
39. This study report that protein levels of the p70 S6 kinase was increased in Progressive Supranuclear Palsy and Corticobasal Degeneration brains. PMID: 26818518
40. Collectively, our findings suggested that both in vitro and in vivo differentiation of Th17 cells were positively regulated by p70(S6K1) PMID: 26514620
41. Our results suggest that silencing of AT1R inhibits EMT induced by HG in HK-2 cells via inactivation of mTOR/p70S6K signaling pathway. PMID: 26626074
42. Results suggest that blocking both the mTOR kinase downstream targets 4E-BP1 protein and p70 S6 kinase 1, but not p70 S6 kinase 1 alone, prevents the migration of retinal pigment epithelium (RPE) cells. PMID: 26427479
43. Our study indicated that Microcystin-LR exposure promoted HL7702 cell proliferation and the main mechanism was the activation of Akt/S6K1 cascade. PMID: 26506538
44. This is the first study highlighting the activation of S6K1 by palmitic acid as a common and novel mechanism by which its inhibition by oleic acid prevents endoplasmic reticulum stress, lipoapoptosis and insulin resistance in hepatocytes. PMID: 25846498
45. These data suggest that S6K1 may represent a molecular link between aging and Alzheimer disease. PMID: 26468204
46. The increased level of S6K1 is positively associated with obesity, insulin resistance and inflammation. PMID: 25118997
47. mTORC1 regulates cell adhesion through S6K1 and 4E-BP1 pathways, but mTORC2 regulates cell adhesion via Akt-independent mechanism PMID: 25762619
48. pS6 expression is associated with the characteristics of a high Ki-67 subset in ER+ and HER2- breast cancer whose proliferation seemed to be affected by activation possibly of the mTOR/S6 pathway. PMID: 25600244
49. Data show that leucine alone stimulates mTORC1 signaling and ribosomal protein s6 kinase 1 (S6K1) phosphorylation. PMID: 26169935
50. Inactivated Sendai virus induces apoptosis and autophagy via the PI3K/Akt/mTOR/p70S6K pathway in human non-small cell lung cancer cells. PMID: 26235873
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亞細(xì)胞定位：

Cell junction, synapse, synaptosome. Mitochondrion outer membrane. Mitochondrion. Note=Colocalizes with URI1 at mitochondrion.; [Isoform Alpha I]: Nucleus. Cytoplasm.; [Isoform Alpha II]: Cytoplasm.
蛋白家族：

Protein kinase superfamily, AGC Ser/Thr protein kinase family, S6 kinase subfamily
組織特異性：

Widely expressed.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 10436

OMIM: 608938

KEGG: hsa:6198

STRING: 9606.ENSP00000225577

UniGene: Hs.463642

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