Histone H2B type 1-K Recombinant Monoclonal Antibody
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中文名稱：組蛋白 H2B type 1-K重組抗體
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貨號：CSB-RA010411A0HU
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規(guī)格：￥1320
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圖片：
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Western Blot
Positive WB detected in: Mouse brain tissue, Rat liver tissue, Rat lung tissue
All lanes: Histone H2B type 1-K antibody at 0.16μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 KDa
Observed band size: 14 KDa -
IHC image of CSB-RA010411A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CSB-RA010411A0HU為Histone H2B type 1-K重組單克隆抗體，靶向真核生物染色質(zhì)核心組分組蛋白H2B家族成員，該蛋白通過與DNA及其他組蛋白協(xié)同組裝核小體，參與維持基因組穩(wěn)定性和表觀遺傳調(diào)控。本抗體經(jīng)多種實驗驗證，在Western Blot中展現(xiàn)出高特異性，可清晰檢測目標(biāo)蛋白條帶，推薦使用1:5000-1:10000稀釋度；在免疫組化（IHC）中能有效定位細胞核內(nèi)信號，建議稀釋度為1:50-1:500。此外，該抗體亦適用于ELISA檢測，為研究者提供多平臺兼容性。其低交叉反應(yīng)性設(shè)計確保了對Histone H2B type 1-K亞型的高度選擇性，適用于染色質(zhì)結(jié)構(gòu)分析、表觀遺傳修飾研究及基因表達調(diào)控機制探索等科研領(lǐng)域，可為細胞周期、DNA損傷修復(fù)或轉(zhuǎn)錄調(diào)控相關(guān)實驗提供可靠工具。
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Uniprot No.：
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基因名：HIST1H2BK
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別名：H2BC12 antibody; H2BFT antibody; HIRIP1 antibody; HIST1H2BK antibody; Histone H2B type 1-K antibody; H2B K antibody; HIRA-interacting protein 1 antibody
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反應(yīng)種屬：Human
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免疫原：A synthesized peptide
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：4G10
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IHC
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推薦稀釋比：
Application Recommended Dilution WB 1:5000-1:10000 IHC 1:50-1:500 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情
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功能：Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.; Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.
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基因功能參考文獻：
- Histone H2B antimicrobial peptides exhibit antibacterial activity and contribute to the formation of the antimicrobial barrier of the colonic epithelium PMID: 15019208
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亞細胞定位：Nucleus. Chromosome.
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蛋白家族：Histone H2B family
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數(shù)據(jù)庫鏈接：
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