HDAC2 Recombinant Monoclonal Antibody

說明書

中文名稱：

HDAC2重組抗體
貨號：

CSB-RA593846A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate
All lanes: HDAC2 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 56, 52 kDa
Observed band size: 60 kDa

IHC image of CSB-RA593846A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

HDAC2 Recombinant Monoclonal Antibody（CSB-RA593846A0HU）是一款特異性識別組蛋白去乙酰化酶2（HDAC2）的高靈敏度抗體，適用于多種蛋白檢測實驗。HDAC2作為表觀遺傳調控的核心成員，通過介導組蛋白去乙酰化參與染色質重塑、基因沉默及細胞周期調控，其異常表達與腫瘤發生、神經退行性疾病及代謝紊亂密切相關。經嚴格驗證，該抗體在Western Blot（WB）中可精準識別天然及重組HDAC2蛋白，推薦使用稀釋度為1:500-1:5000；在免疫組化（IHC）實驗中能清晰呈現組織樣本中HDAC2的亞細胞定位，推薦稀釋度為1:50-1:200，同時支持ELISA平臺檢測。其高特異性和批次一致性已通過多種實驗體系驗證，可滿足表觀遺傳學機制研究、癌癥生物學探索及神經科學領域的基礎科研需求，適用于細胞模型、組織切片及體外蛋白分析等場景，為研究HDAC2在疾病相關信號通路中的功能提供可靠工具。本產品僅限科學研究使用。
Uniprot No.：

Q92769
基因名：

HDAC2
別名：

Histone deacetylase 2 (HD2) (EC 3.5.1.98), HDAC2
反應種屬：

Human
免疫原：

A synthesized peptide derived from human HDAC2
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

9E9
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A.
基因功能參考文獻：
1. Results found that HDAC2 expression is up-regulated in patients with chronic diabetic foot ulcer and in high glucose induced endothelial progenitor cells. PMID: 30063937
2. Comparative molecular docking studies of the lead RH01652 with class I HDACs (HDAC1, HDAC2, HDAC3, and HDAC8) shows higher binding affinity towards HDAC2. Thus, lead RH01652 could serve as template to design novel and potent inhibitor of HDAC2. PMID: 29932788
3. human immortalized telencephalic/mesencephalic microglial cells reveal significant upregulation of HDAC2 in the presence of the potent microglial activator lipopolysaccharide PMID: 29803514
4. we found that HDAC2 was the direct target of miR-31 by binding to 3'-UTR from the results of luciferase reporter assays, qRT-PCR, and western blotting. HDAC2 played an activation role in tumor growth, whose expression is upregulated and inversely associated with miR-31 levels PMID: 29333444
5. data indicate that the obesity impacts on H4ac levels and that strenuous exercise leads to an enhanced chronic low-grade inflammation profile in obesity via an imbalance on H4ac/HDAC2. PMID: 29142617
6. Knockdown of HDAC2 completely mimicked the effects of 1,25(OH)2D3 on PTEN gene expression. PMID: 28737824
7. Muscle biopsies of chronic obstructive pulmonary disease significantly correlated with HDAC2 decrease compared to controls. PMID: 28526090
8. With tissue microarrays of hepatocellular carcinoma (HCC) patients, we determined the prognostic values of the core genes in the network and found that RAD21, CDK1, and HDAC2 expression levels were negatively associated with overall survival for HCC patients. PMID: 28434945
9. Combined treatment with the histone deacetylase inhibitors (HDACi) suberoylanilide hydroxamic acid plus 5-fluorouracil (5-FU) and oxaliplatin (Oxa) reduced the level of HDAC2 expression in HT-29 cells. PMID: 27283986
10. miR-223 controls the expression of CX3CL1 by targeting HDAC2 in chronic obstructive pulmonary disease patients and mouse models of the disease. PMID: 26864305
11. Data show that BRCA2 was required for HDAC2/3 association with acetylated BubR1 in nocodazole (Noc)-arrested cells. PMID: 28985013
12. study defines two classes of HDAC2 targets in human cells, with a dependence of HDAC1 on HDAC2 at one class of targets, and distinguishes unique functions for HDAC2 PMID: 28982113
13. This study demonstrated that the Expression of HDAC2 Transcript Is Reduced in Dorsolateral Prefrontal Cortex of Patients with Schizophrenia. PMID: 27959513
14. The findings suggest that miR-455-3p plays a critical role during chondrogenesis by directly targeting HDAC2/8 and promoting histone H3 acetylation. PMID: 27638301
15. report the interaction between CFTR and HDAC2, and its involvement in the development of Ph+ leukemia PMID: 28235656
16. USP4 inhibits p53 and NF-kappaB through deubiquitinating and stabilizing HDAC2 PMID: 26411366
17. HDAC2 controls ciliogenesis independently of Kras, which facilitates Aurora A expression. These studies suggest that HDAC2 is a novel regulator of primary cilium formation in PDAC cells. PMID: 28028031
18. the current findings implicate the HDAC2/miR-101/AMPK pathway as a critical mediator of AD pathogenesis. These studies also highlight the importance of epigenetics in AD and provide novel therapeutic targets. PMID: 28202389
19. HDAC2 upregulation is associated with hepatocellular carcinoma. PMID: 27342975
20. Suggest that HDAC2 can trigger migration and invasion of non-small cell lung carcinoma cells by activating NF-kappaB to up-regulate fibronectin expression. PMID: 27665474
21. KLF4 acts as a tumor suppressor or oncogene to activate or repress target gene transcription depending on its acetylation status, which is regulated by p21 and CK2 interaction-mediated HDAC2 phosphorylation PMID: 26729194
22. Decreased HDAC2 expression is associated with cisplatin resistance in ovarian cancer. PMID: 26683361
23. In cigarette smoke extract-exposed airway epithelial cells and macrophages, HDAC2 is excessively ubiquitinated and degraded in the proteasome attributed to low expression of USP17. PMID: 26617781
24. The results imply that HDAC2 is involved in the transcriptional regulation of human odontoblasts in vivo. PMID: 22297573
25. Endothelial-Mesenchymal Transition is initiated by recruitment of aberrantly phosphorylated DNMT1 to the RASAL1 CpG island promoter by HDAC2, causing aberrant promoter methylation and transcriptional suppression. PMID: 26815200
26. Selective inhibition of HDAC2 in lung tumor cell causes survivin downregulation through activation of p53, which is mediated by downregulation of Mdm2. PMID: 25605253
27. our study revealed that HDAC2 is overexpressed in colorectal cancer (CRC) cells; its knockdown can increase the sensitivity of CRC cells to doxorubicin via upregulation of ABCB1 PMID: 26846508
28. Pancreatic adenocarcinoma patients with enhanced HDAC-1 and -6 expression showed significantly longer survival times compared to those with low expression, while a borderline association concerning HDAC-2 expression was noted. PMID: 26502922
29. Lymphocyte senescence in COPD is associated with loss of HDAC2 in CD28nullCD8+ T and NKT-like cells. PMID: 26498345
30. HDAC2 may not confer susceptibility to Schizophrenia in Han Chinese. PMID: 26063464
31. In endothelial dysfunction, HDAC2 levels were reciprocally regulated by ectopic expression of NEDD8 and the de-NEDDylating enzyme SENP8. PMID: 25655932
32. Aberrant overexpression of HDACs in basal cells of IPF lungs may contribute to the bronchiolisation process in this disease. Similarly, generation and apoptosis resistance of IPF fibroblasts are mediated by enhanced activity of HDAC enzymes. PMID: 26359372
33. HDAC2 sumoylation is important for NF-kappaB-dependent gene expression and for the resistance of cell against stress PMID: 25704882
34. HDAC2 inhibition reduces proliferation and induces apoptosis via the caspase-dependent pathway in human glioma cell lines, possibly by activating the p53 signaling pathway. PMID: 25523932
35. Data indicate that that hstone deacetylase 2 (HDAC2) has a specific role in leukemogenesis. PMID: 25473896
36. HDAC3 is an essential target to disrupt HIV-1 latency, and inhibition of HDAC2 may also contribute to the effort to purge and eradicate latent HIV-1 infection. PMID: 25136952
37. BRG1/HDAC2 and beta-catenin constitute a manipulative apparatus at the transcription start site to play opposite but complementary roles in regulating hTERT expression. PMID: 25486475
38. HDAC2 plays a central role in coupling lysine acetylation to synaptic plasticity and mediates many of the effects of HDAC inhibition in cognition and disease. PMID: 25492968
39. Data suggest that p15RS (p15INK4b-related sequence) acts as an intrinsic transcriptional repressor for Wnt/beta-catenin-mediated gene transcription through recruiting HDAC2 histone deacetylase. PMID: 25697359
40. Simultaneous loss of Hdac1 and Hdac2 resulted in loss of hematopoietic stem cells. PMID: 24763403
41. our data suggested that cPA may have beneficial effects in inflammation-related cardiovascular disease by controlling HDAC2 regulation PMID: 25013374
42. HDAC2 silencing in HCC cells also strongly inhibited PPARgamma signaling. PMID: 24958469
43. inhibitory of cell growth by melittin might be led by HDAC2-mediated PTEN upregulation, Akt inactivation, and inhibition of the PI3K/Akt signaling pathways PMID: 24788349
44. Osteocalcin levels were decreased, an effect induced at the transcriptional level, and were strongly correlated with inhibition of HDAC2. PMID: 24105979
45. these results indicated that the potential of specific inhibition of HDAC2 by small molecular chemicals may lead to future therapeutic agents in human renal cancer treatment. PMID: 24390319
46. The proliferation inhibition and cell cycle arrest mediated by downregulated HDAC2 expression may be tightly associated with the decrease of cyclin D1, cyclin E, and cdk2 proteins expression. PMID: 24965412
47. PELP1 regulates tumor metastasis by controlling the expression and functions of the tumor metastasis suppressors miR-200a and miR-141 PMID: 23975430
48. HDAC2 is a critical regulator of Arg2 expression and thereby endothelial nitric oxide and endothelial function. PMID: 24833798
49. Data indicate prominent changes in urothelial cancer cell lines (UCC) were HDAC2 and/or HDAC8 up-regulation. PMID: 22944197
50. our study has found no association between HDAC2/HDAC3 gene polymorphisms and schizophrenia in the Chinese Han population PMID: 23857786
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亞細胞定位：

Nucleus. Cytoplasm.
蛋白家族：

Histone deacetylase family, HD type 1 subfamily
組織特異性：

Widely expressed; lower levels in brain and lung.
數據庫鏈接：

HGNC: 4853

OMIM: 605164

KEGG: hsa:3066

STRING: 9606.ENSP00000430432

UniGene: Hs.3352

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