CCNE1 Recombinant Monoclonal Antibody

說明書

中文名稱：

CCNE1重組抗體
貨號：

CSB-RA968740A0HU
規格：

￥1320
圖片：
IHC image of CSB-RA968740A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA968740A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of Hela Cells with CSB-RA968740A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CCNE1 Recombinant Monoclonal Antibody（CSB-RA968740A0HU）是針對細胞周期調控關鍵蛋白CCNE1開發的高特異性檢測工具。該抗體靶向的CCNE1蛋白通過調控G1/S期轉換參與細胞周期進程，其異常表達與乳腺癌、卵巢癌等多種惡性腫瘤的發生發展密切相關。本產品經ELISA、免疫組化（IHC）和免疫熒光（IF）等多平臺驗證，在IHC應用中推薦使用1:50-1:200稀釋比例，IF實驗建議采用1:20-1:200稀釋范圍，實驗數據顯示其能清晰識別石蠟切片中的抗原定位及細胞內的特異性熒光信號，且表現出良好的批次間一致性和重復性。適用于腫瘤發生機制研究、細胞周期相關信號通路分析、癌癥生物標志物檢測以及靶向治療藥物開發等科研領域，特別推薦用于臨床前模型中腫瘤組織樣本的蛋白表達水平檢測及亞細胞定位研究。該抗體采用重組單克隆技術制備，具有高親和力和低交叉反應性優勢，可為癌癥基礎研究提供可靠的實驗支持。
Uniprot No.：

P24864
基因名：

CCNE1
別名：

G1/S-specific cyclin-E1, CCNE1, CCNE
反應種屬：

Human
免疫原：

A synthesized peptide derived from human Cyclin E1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

3A5
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:20-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Essential for the control of the cell cycle at the G1/S (start) transition.
基因功能參考文獻：
1. miR-874 inhibits CCNE1 expression during growth factor deprivation and that miR-874 down-regulation in osteosarcomas leads to CCNE1 up-regulation and more aggressive growth phenotypes. PMID: 29109143
2. Amplified/cyclin E1(hi) and non-amplified/cyclin E1(hi) tumors have different pathological and biological characteristics and clinical outcomes indicating that they are separate subsets of cyclin E1(hi) HGSOC. PMID: 30209015
3. Breast cancer recurrence-free interval was significantly worse in patients with cyclin E (LMW-E)-positive tumors who received aromatase inhibitor (AI) neoadjuvant therapy, compared with those with LMW-E negative tumors. PMID: 28947566
4. in a series of human biopsies, non-metastatic SCCs displayed a higher degree of chromosomal alterations and higher expression of the S phase regulator Cyclin E and the DNA damage signal gammaH2AX than the less aggressive, non-squamous, basal cell carcinomas. However, metastatic Squamous cell carcinoma lost the gammaH2AX signal and Cyclin E, or accumulated cytoplasmic Cyclin E. PMID: 28661481
5. Cytoplasmic cyclin E identifies patients with the highest likelihood of recurrence consistently across different patient cohorts and subtypes. These patients may benefit from alternative therapies targeting the oncogenic isoforms of cyclin E. PMID: 27881578
6. our results validate the assumption that CBX7 is a tumor suppressor of gliomas. Moreover, CBX7 is a potential and novel prognostic biomarker in glioma patients. We also clarified that CBX7 silences CCNE1 via the combination of CCNE1 promoter and the recruitment of HDAC2. PMID: 28460453
7. Our findings suggest that gene copy-number gain and upregulation of CCNE1 occur in ovarian clear cell carcinoma and are associated with a worse clinical outcome, dictating the survival of early-stage patients. PMID: 27767100
8. YAP/ TAZ pathways contribute to the proliferation/quiescence switch during colon cancer 5FU treatment according to the concerted regulation of Cyclin E1 and CREB PMID: 27527859
9. Silencing of CDCA5 suppresses proliferation of gastric cancer cells by inducing G1-phase arrest via downregulating CCNE1. PMID: 29326043
10. Analysis of genomic data from TCGA demonstrated coamplification of CCNE1 and AKT2 Overexpression of Cyclin E1 and AKT isoforms, in addition to mutant TP53, imparted malignant characteristics in untransformed fallopian tube secretory cells, the dominant site of origin of high-grade serous ovarian cancer PMID: 27663592
11. Finding suggest that amplification of CCNE1 serves as one mechanism for the development of some serous tubal intraepithelial carcinomas. PMID: 27443516
12. Prognostic gene sets based on the 13 genes were developed, and their prognostic values were verified in three independent patient cohorts (n=501). Among them, a signature of CCNE1 and its coexpressed genes was significantly associated with disease progression and validated in the independent cohorts. PMID: 28082741
13. Cyclin E1 mRNA and protein expressions were suppressed. PMID: 26603262
14. The opposing effects of ORC1 (represor) and CDC6 (gene activator) in controlling the level of Cyclin E ensures genome stability and a mechanism for linking directly DNA replication and cell division commitment. PMID: 27458800
15. High cyclin E expression is associated with breast cancer. PMID: 28760857
16. High CCNC1 expression is associated with inflammatory breast cancer. PMID: 28107181
17. Inhibition of cell division cycle associated 2 (CDCA2) suppressed the proliferation of lung adenocarcinoma (LAC) cells via G1 phase arrest by downregulating cyclin E1(CCNE1), while overexpression of CDCA2 promoted LAC cells proliferation by upregulating CCNE1. PMID: 28423619
18. a PI3K/PKCiota/cyclin E signaling pathway as a therapeutic target during ovarian tumorigenesis PMID: 26279297
19. Amplification of 19q12 CCNE1/URI was found in 10.4% (28/270) and was significantly associated with type II endometrial cancer (EC) high grade, advanced FIGO stage, and aberrant tumor supressor p53 expression. PMID: 27582547
20. Results show that cyclin E1 and CDK2 participate in STC1 promoting cell proliferation of prostate neoplasm cells. PMID: 28350121
21. cyclin E is specifically dephosphorylated at S384 by the PP2A-B56 phosphatase, thereby uncoupling cyclin E degradation from cyclin E-CDK2 activity PMID: 28137908
22. These results provide evidence that ARTD1 regulates cell cycle re-entry and G1/S progression via cyclin E expression and p27(Kip 1) stability independently of its enzymatic activity, uncovering a novel cell cycle regulatory mechanism. PMID: 27295004
23. These results demonstrate a repressor role for NFAT1 in cell cycle progression and Cyclin E expression in B lymphocytes, and suggest a potential function for NFAT1 protein in B cell malignancies. PMID: 27399331
24. High CCNE1 amplification and expression is associated with breast cancer. PMID: 26810187
25. CCNE1/REL gene interaction might play pivotal roles in the occurrence and development of Postmenopausal Osteoporosis. PMID: 26676054
26. These results indicate that miR-25 has anti-apoptosis roles in AGS cells, possibly via inhibiting FBXW7 and thus promoting oncogenes, such as CCNE1 and MYC. PMID: 27120728
27. Cyclin E-driven OvCa cells appeared addicted to glucose metabolism via TCA. Combined CDKi with modalities targeting TCA, like SDHA inhibition showed promising effects for this genotype. Combined blockade of CDK and SDH, both genetically and pharmaceutically, showed synergy and resulted in inhibited proliferation, migration, invasion and migration in A2780 cells PMID: 26826064
28. Over-expression of CCNE1 is associated with non-small cell lung cancer. PMID: 26771237
29. High levels of cyclin E are a predicator of poor prognosis among patients with gastrointestinal cancer. [meta-analysis] PMID: 25627202
30. The effects of CCNE1 knockdown were dependent on the CCNE1 expression status. PMID: 26647729
31. Data show that melanoma antigen, family C, 2 protein (MAGE-C2) binds with RING-box protein 1 (Rbx1) and Cullin 1, and regulates cyclin E stability in melanoma cells. PMID: 26540345
32. The findings suggest that the role of cyclin E and tumor specific low molecular weight isoforms as mediators of tumorigenesis is in part dependent on p53 context. PMID: 26625764
33. Ad-cycE can target cyclin E overexpression in cancer cells and repress tumor growth in syngeneic mouse models. PMID: 26475304
34. ovary tumors with elevated CCNE1 expression may be staged for Cdk2-targeted therapy PMID: 26204491
35. our findings identify a novel mechanism of cyclin E-mediated Mcl-1 regulation in human cancer PMID: 26219338
36. miR-15b might be involved in termination of osteoblastic cells proliferation by arresting them at G0/G1 phase through direct targeting cyclin E1. PMID: 26007664
37. A significant correlation between cyclin E1 amplification and deletions at a number of the genomic loci in breast cancer. PMID: 25959964
38. These results indicate cyclin E1 is downregulated by both miR-497 and miR-34a, which synergistically retard the growth of human lung cancer cells. PMID: 25909221
39. MicroRNA-30c-2-3p negatively regulates NF-kappaB signaling and cell cycle progression through downregulation of TRADD and CCNE1 in breast cancer. PMID: 25732226
40. Results show that miR-16 and HuR co-regulate the cyclin E1 mRNA without influencing each other's binding or expression. miR-16 regulation predominates, blocking upregulation of cyclin E1 by HuR. PMID: 25830480
41. Expression of Notch1, -2, and -3, CDK2, and CCNE1 was significantly decreased by upregulation of ALDH1A1 in A549 cells, but increased by its interruption in A549s cells. PMID: 24671051
42. Results show that CCNE1 and BRD4 on chromosome 19 were amplified/overexpressed in a substantial cases of epithelial ovarian cancer with no involvement of BRCA genes. PMID: 25892415
43. This study indicates that CCNE1 rs1406 polymorphism may contribute to BC risk PMID: 25159285
44. Suppression of NF90 caused a decrease in the half-life of cyclin E1 mRNA PMID: 25399696
45. miR-144-5p functions as tumour suppressor in BC cells. CCNE1 and CCNE2 were directly regulated by miR-144-5p and might be good prognostic markers for survival of bladder cancer patients PMID: 26057453
46. HOXA7 promotes cell proliferation, and these changes are mediated by cyclin E1/CDK2 PMID: 25501982
47. Aurora-A/HURP relays cell transforming signal to NF-kappaB, and the HURP/NF-kappaB complex is engaged in the regulation of cyclin E1 expression. PMID: 25289861
48. Contrary to previous literature, we found a correlation between cyclin E expression and prognosis. Further large-scale studies are required to confirm our findings. PMID: 26026100
49. Global gene expression profiling identifies ALDH2, CCNE1 and SMAD3 as potential prognostic markers in upper tract urothelial carcinoma. PMID: 25408144
50. The cell cycleassociated proteins cyclin E and p27kip1 may have contributed to this antitumor effect. PMID: 25310086
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亞細胞定位：

Nucleus.
蛋白家族：

Cyclin family, Cyclin E subfamily
組織特異性：

Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
數據庫鏈接：

HGNC: 1589

OMIM: 123837

KEGG: hsa:898

STRING: 9606.ENSP00000262643

UniGene: Hs.244723

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