CASP2 Recombinant Monoclonal Antibody

說明書

中文名稱：

CASP2重組抗體
貨號：

CSB-RA782200A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: K562 whole cell lysate, 293 whole cell lysate
All lanes: Caspase-2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 51, 35, 11 kDa
Observed band size: 51 kDa

IHC image of CSB-RA782200A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA782200A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CASP2 Recombinant Monoclonal Antibody（CSB-RA782200A0HU）是針對半胱天冬酶2（Caspase-2）開發的高特異性抗體，該靶點作為細胞凋亡執行蛋白家族成員，在調控程序性細胞死亡、維持基因組穩定性及DNA損傷應激響應中發揮重要作用。本抗體經多種實驗嚴格驗證，可在ELISA實驗中實現精準的抗原結合檢測，在Western Blot中高效識別天然及重組CASP2蛋白，并在免疫組化（IHC）中清晰呈現組織樣本中亞細胞水平的表達定位。優化后的工作濃度范圍（WB 1:500-1:5000；IHC 1:50-1:200）可滿足不同實驗體系需求，其低背景信號和高批次穩定性確保實驗結果的重復性。該產品適用于探究細胞凋亡信號通路調控機制、DNA損傷修復研究、腫瘤發生相關蛋白互作分析，以及神經退行性疾病模型中CASP2的功能解析。通過嚴格的交叉反應性測試驗證，本抗體對同源蛋白無顯著交叉識別，為體外培養細胞、組織切片等樣本的靶蛋白檢測提供可靠工具。
Uniprot No.：

P42575
基因名：

CASP2
別名：

Caspase-2 (CASP-2) (EC 3.4.22.55) (Neural precursor cell expressed developmentally down-regulated protein 2) (NEDD-2) (Protease ICH-1) [Cleaved into: Caspase-2 subunit p18, Caspase-2 subunit p13, Caspase-2 subunit p12], CASP2, ICH1 NEDD2
反應種屬：

Human
免疫原：

A synthesized peptide derived from human Caspase-2
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

3D4
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. Associates with PIDD1 and CRADD to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis in response to genotoxic stress.
基因功能參考文獻：
1. these data identify a novel caspase-2-interacting factor, FAN, and expand the role for the enzyme in seemingly non-apoptotic cellular mechanisms. PMID: 29621545
2. Results suggest that TG-induced macrophage cell death is mediated via the caspase-2. PMID: 28768565
3. study demonstrates that apoptosis inhibitor 5 (API5/AAC11) is an endogenous and direct inhibitor of caspase-2. API5 protein directly binds to the caspase recruitment domain (CARD) of caspase-2 and impedes dimerization and activation of caspase-2. PMID: 28336776
4. This peptide, Ac-VDTTD-AFC, was efficiently cleaved by purified caspase-2 and auto-activating caspase-2 in mammalian cells, and exhibited better selectivity for caspase-2 relative to caspase-3 than reagents that are currently available. PMID: 27919034
5. There results support a special role for miR-149 in malignant glioma by targeting Caspase-2 PMID: 27049919
6. Whole exome sequencing (WES) of an affected fetus, and subsequent Sanger sequencing of the second fetus, revealed a homozygous frameshift variant in CRADD, which encodes an adaptor protein that interacts with PIDD and caspase-2 to initiate apoptosis PMID: 28686357
7. This study shows that human procaspase-2 interaction with 14-3-3 zeta is governed by phosphorylation at both S139 and S164. PMID: 28943433
8. NPM1-dependent nucleolar PIDDosome is a key initiator of the caspase-2 activation cascade. PMID: 28432080
9. Sensitization of colon carcinoma cells to radiation-induced cell death and DNA-damage by HuR knockdown critically depends on caspase-2. PMID: 28219770
10. BCL9L dysfunction contributes to aneuploidy tolerance in both TP53-WT and mutant cells by reducing basal caspase-2 levels and preventing cleavage of MDM2 and BID. PMID: 28073006
11. CASP2 down-regulation had a reverse relationship with miR-383 down-regulation in regulating epithelial ovarian cancer development. PMID: 27567588
12. Results suggest that mutations at all three cleavage sites of caspase-2 protein neither affect the macromolecular core complex assembly, nor modify caspase-2 activity upon DNA damage. Consequently, caspase-2 activation occurs in the macromolecular complex without its dissociation. PMID: 27193717
13. These findings indicate that miR-125a-5p decreases after HOTAIR knockdown to promote cancer cell apoptosis by releasing caspase 2. PMID: 26962687
14. these studies elucidate a Caspase-2-p53 signaling network that impacts lung tumorigenesis and chemotherapy response in vivo. PMID: 25301067
15. We have also demonstrated that these correlations are tissue specific being reduced (CASP9 and CASP10) or different (CASP2) in the liver PMID: 25330190
16. the initiator caspase-2 is required for robust death of ovarian cancer cells induced by FASN inhibitors PMID: 25151963
17. HuR sensitizes adenocarcinoma cells to the intrinsic apoptotic pathway by upregulating the translation of caspase-2. PMID: 25010987
18. Authors have demonstrated in vitro and in vivo that loss of function of caspase-2 allows to escape oncogenic stress induced senescence. PMID: 25114039
19. Data strongly argue against a critical role for caspase-2 in ER-stress-induced apoptosis. PMID: 24292555
20. axon regeneration promoted by suppression of CASP2 and CASP6 is CNTF-dependent and mediated through the JAK/STAT signalling pathway PMID: 24727569
21. Our results reveal a novel mechanism of caspase-2 pre-mRNA splicing. PMID: 24321384
22. TRIM16 can promote apoptosis by directly modulating caspase-2 activity in cancer cells. PMID: 23404198
23. The role of caspase-2 isoforms in the progression of breast cancer may considerably differ between pre- and post-chemotherapy patients. PMID: 23469978
24. MiR-708 may act as an oncogene and induce the carcinogenicity of bladder cancer by down-regulating Caspase-2 level. PMID: 23568547
25. caspase-2 has a role as an initiator caspase in lipoapoptosis PMID: 23553630
26. Data indicate induction of caspase-2 by sorting nexin 5 (SNX5) in papillary thyroid carcinoma PMID: 22486813
27. activated human caspase-2 shares remarkably overlapping protease specificity with the prototype apoptotic executioner caspases-3 and -7, suggesting that caspase-2 could function as a proapoptotic caspase once released from the activating complex. PMID: 22825847
28. IRE1alpha regulates translation of a proapoptotic protein, Caspase-2, through terminating microRNA biogenesis, and noncoding RNAs are part of the ER stress response PMID: 23042294
29. These results revealed a thus far unknown, obligatory role for caspase-2 as an initiator caspase during pore-forming toxins -mediated apoptosis. PMID: 22531785
30. caspase-2 acts upstream of caspase-3 and that caspase-2 functions in response to DNA damage in both PhSe-T- and MeSe-T-induced apoptosis. PMID: 22002103
31. Tumor-suppressing function of caspase-2 requires catalytic site Cys-320 and site Ser-139 in mice. PMID: 22396545
32. TAp73alpha represses caspase-2 enzymatic activity and by this means reduce caspase-2 induced Bax activation, loss of mitochondrial transmembrane potential and resulting apoptosis in small cell lung carcinoma cells. PMID: 22201672
33. Data suggest that this novel role of caspase-2 as a translational regulator of p21 expression occurs not only independently of its enzymatic activity but also does not require known caspase-2-activating platforms. PMID: 21475302
34. Findings suggest that XPC enhances DNA damage-induced apoptosis through inhibition of caspase-2 (casp-2S) transcription. PMID: 22174370
35. Studies indicate that DNA damage may trigger caspase 2 activation. PMID: 22077397
36. somatic mutation of caspase-2 is rare in gastric and colorectal carcinomas. PMID: 21940110
37. ras-induced down-regulation of caspase-2 represents a novel mechanism by which oncogenic Ras protects malignant intestinal epithelial cells from anoikis PMID: 21903589
38. Structural and enzymatic insights into caspase-2 protein substrate recognition and catalysis. PMID: 21828056
39. Caspase-2 directly cleaves the E3 ubiquitin ligase Mdm2 at Asp 367, leading to loss of the C-terminal RING domain responsible for p53 ubiquitination. PMID: 21726810
40. analyzed cancer tissues from acute leukemias, breast cancers, lung cancers, and liver cancers for the detection of caspase-2 somatic mutations PMID: 21332795
41. The changes of caspase-2 and caspase-5 activities could be indicative of their involvement in the cervical malignancy mechanisms. PMID: 21051981
42. Activation of caspase-9, but not caspase-2 or caspase-8, is essential for heat-induced apoptosis in Jurkat cells. PMID: 20978129
43. This review discusses recent advances that have been made to help elucidate the true role of caspase 2 and the potential contribution of caspase-2 to the pathology of human diseases including cancer. PMID: 20158568
44. caspase-2 activation is commonly associated with induction of IFN-beta-induced apoptosis in IFN-beta-sensitive melanoma cells PMID: 20187765
45. we suggest that caspase-2 and/or -8 plays an important role in regulating gamma-secretase and may possibly provide a basis for the development of therapeutics targeting apoptosis PMID: 20143425
46. The expressions of PIDD and RAIDD are upregulated during tumour progression in renal cell carcinomas. PMID: 20208132
47. caspase 2 functions as an endogenous inhibitor of NFkappaB-dependent cell survival and this mechanism may contribute to tumor suppression in humans. PMID: 19935698
48. Data show that the N-t-boc-Daidzein induced apoptosis is characterized by caspase activation, XIAP and AKT degradation. PMID: 19738422
49. Data indicate that caspase activity was not essential for docetaxel-induced cytotoxicity since cell death associated with lysosomal membrane permeabilization still occurred in the presence of caspase inhibitors. PMID: 19715609
50. role in cytochrome C release and apoptosis from the nucleus PMID: 11823470
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蛋白家族：

Peptidase C14A family
組織特異性：

Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.
數據庫鏈接：

HGNC: 1503

OMIM: 600639

KEGG: hsa:835

STRING: 9606.ENSP00000312664

UniGene: Hs.368982

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