BUB1 Recombinant Monoclonal Antibody

說明書

中文名稱：

BUB1重組抗體
貨號：

CSB-RA281837A0HU
規(guī)格：

￥1320
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate
All lanes: Bub1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 123, 116, 120 kDa
Observed band size: 130 kDa
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

CSB-RA281837A0HU BUB1重組單克隆抗體是針對有絲分裂檢查點關(guān)鍵調(diào)控因子BUB1研發(fā)的高特異性檢測工具。該抗體靶向的BUB1蛋白在細胞周期調(diào)控中發(fā)揮重要作用，通過參與紡錘體組裝檢查點（SAC）機制確保染色體精確分離，其功能異常與基因組不穩(wěn)定性及腫瘤發(fā)生密切相關(guān)。經(jīng)嚴格驗證，本產(chǎn)品在Western Blot（WB）實驗中能特異性識別內(nèi)源性BUB1蛋白，在推薦1:500-1:5000稀釋范圍內(nèi)呈現(xiàn)清晰單一的目標條帶，且背景干擾低；同時適用于ELISA平臺檢測，展現(xiàn)優(yōu)異的抗原結(jié)合能力。采用重組單克隆抗體技術(shù)生產(chǎn)，確保批次間高度一致性，經(jīng)多指標質(zhì)控驗證其靈敏度與穩(wěn)定性。該抗體適用于細胞周期調(diào)控機制、有絲分裂檢查點功能、染色體分離異常等相關(guān)研究領(lǐng)域，可應(yīng)用于腫瘤細胞模型、基因編輯細胞系或藥物處理樣本中BUB1蛋白表達的定量檢測及動態(tài)變化分析，為探索基因組穩(wěn)定性調(diào)控網(wǎng)絡(luò)提供可靠工具。本產(chǎn)品嚴格限定于科學(xué)研究用途。
Uniprot No.：

O43683
基因名：

BUB1
別名：

Mitotic checkpoint serine/threonine-protein kinase BUB1 (hBUB1) (EC 2.7.11.1) (BUB1A), BUB1, BUB1L
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from human Bub1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

9C11
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.
基因功能參考文獻：
1. Conserved domain 1 (CD1) in human Bub1 binds directly to Mad1 and a phosphorylation site exists in CD1 that stimulates Mad1 binding and SAC signalling. PMID: 28604727
2. Finally, the authors show that the kinetochore localization of BUB1 and BUBR1 decreases with the age of the oocyte donors. This could contribute to oocyte aneuploidy. PMID: 28287092
3. Small sequence differences in Bub1 and BubR1 direct Bub3 to different phosphorylated targets in the spindle assembly checkpoint signaling cascade. PMID: 28943088
4. MiR-490-5p could regulate TGFbeta/Smad signaling pathways by inhibiting BUB1. PMID: 28810242
5. Therefore, Mps1 promotes checkpoint activation through sequentially phosphorylating Knl1, Bub1, and Mad1. This sequential multi-target phosphorylation cascade makes the checkpoint highly responsive to Mps1 and to kinetochore-microtubule attachment. PMID: 28072388
6. Chromosomal rearrangement resulting in BUB1 haploinsufficiency identified in a patient with polycystic liver disease. BUB1 may imply germline causes of genetic instability in PLD. PMID: 27552964
7. Results report that BubR1 and RepoMan bind directly to PP2A-B56 using an LSPIxE short linear motif (SLiM), where phosphorylation of the Ser residue enhances binding. RepoMan and BubR1 bind B56 using both hydrophobic and electrostatic interactions. PMID: 27998540
8. Inhibition of Bub1 kinase impaired chromosome arm resolution but exerted only minor effects on mitotic progression or spindle assembly checkpoint function. PMID: 26885717
9. Study shows that BUB1beta and PDZ binding kinase are up-regulated in breast cancer tumors and their expression is associated with improved overall survival (OS) in basal-like tumors but worse OS in luminal and untreated patients. PMID: 26897635
10. Bub1 may be associated with cancer stem cell potential. PMID: 26522589
11. Both Bub1 and BubR1 bind stably to Bub3 that is required for kinetochore localization of the proteins PMID: 26260062
12. Bub1-Plk1-mediated phosphorylation of Cdc20 constitutes an anaphase-promoting complex or cyclosome-inhibitory mechanism that is parallel, but not redundant, to mitotic checkpoint complex formation. PMID: 26912231
13. Data show that mitotic checkpoint kinase Bub1 is an active kinase regulated by intra-molecular phosphorylation at the P+1 loop. PMID: 26658523
14. human BUB1 does not associate stably with the MAD2 activator MAD1 (also known as MAD1L1) and, although required for accelerating the loading of MAD1 onto kinetochores, BUB1 is dispensable for the maintenance of steady-state levels of MAD1 there PMID: 26148513
15. MAD2L1 and BUB1 may play important roles in breast cancer progression, and measuring the expression of these genes may assist the prediction of breast cancer prognosis. PMID: 26287798
16. Data provide novel insight into the regulation and kinetochore residency of Bub1 and indicate that its localization is dynamic and tightly controlled through feedback autophosphorylation. PMID: 26399325
17. Bub1 coordinates checkpoint signalling by distinct domains for RZZ and BubR1 recruitment and localizes antagonistic checkpoint activities. PMID: 26031201
18. BUB1B expression was highly correlated to CDC20 and CCNB1 expression in multiple myeloma cells, leading to increased cell proliferation.Bub1 expression promotes cell proliferation and leads to poor survival in multiple myeloma patients PMID: 25698537
19. BubR1 is a component of the Mitotic checkpoint complex and was reported to be overexpressed in Breast cancer. BubR1 overexpression was correlated with poor survival in early stage Breast cancer patients. PMID: 25731686
20. Bub1 in complex with LANA recruits PCNA to regulate Kaposi's sarcoma-associated herpesvirus latent replication and DNA translesion synthesis. PMID: 26223641
21. Study provides structural and functional insights into the regulation of the key mitotic kinase Bub1 and highlights a mode of substrate-specific kinase activation through intramolecular autophosphorylation of the substrate binding loop. PMID: 25308863
22. The kinase activity of the Ser/Thr kinase BUB1 promotes TGF-beta signaling. PMID: 25564677
23. Data show sequential multisite regulation of the microtubule-associated protein KNL1-kinase-adaptor complex BUB1/BUB3 interaction. PMID: 25661489
24. Bub1, but not BubR1, enhances binding of Bub3 to phosphorylated kinetochores. PMID: 25611342
25. ABBA motifs in BUBR1 and BUB1 are necessary for the spindle assembly checkpoint to work at full strength and to recruit CDC20 to kinetochore PMID: 25669885
26. The bioinformatics analysis suggested that this variant may regulate the transcriptional ability of BUB1 PMID: 24711138
27. BUB1 is co-expressed with AURKA and AURKB suggesting biological relationship between these spindle cell components in primary and metastatic advanced-stage ovarian serous carcinoma PMID: 24756216
28. BUB1 and BUBR1 inhibition decreases proliferation and shows radiosensitizing effects on pediatric glioblastoma cells PMID: 23728478
29. Bub1-mediated H2A phosphorylation penetrates kinetochores and that this histone mark contributes to a tension-sensitive Sgo1-based molecular switch for chromosome segregation. PMID: 24055156
30. Studied the significance of budding uninhibited by benzimidazoles-1 (Bub1) and mitotic arrest deficient-2 (Mad2) expression in endometrial carcinoma. PMID: 24654460
31. Mutations in BUB1 and BUB3 cause mosaic variegated aneuploidy and increase the risk of colorectal cancer at a young age. PMID: 23747338
32. These results all indicated that BUB1 played important roles in the proliferation and/or progression of the breast carcinoma, and nuclear BUB1 immunohistochemical status is also considered a potent prognostic factor in human breast cancer patients. PMID: 23288590
33. IRAK1, CHEK1 and BUB1 may play an important role in ovarian cancer. PMID: 23383610
34. the molecular mechanism of DNA damage-induced Bub1 activation and highlight a critical role of Bub1 in DNA damage response PMID: 22071147
35. ATM-mediated Bub1 Ser314 phosphorylation is required for Bub1 activity and is essential for the activation of the spindle checkpoint. PMID: 22099307
36. Bub1 expression could be regulated by estradiol and paclitaxel in endometrial carcinoma Ishikawa cells. PMID: 21092550
37. Results establish that Bub1 has oncogenic properties and suggest that Aurora B is a critical target through which overexpressed Bub1 drives aneuploidization and tumorigenesis. PMID: 21646403
38. Findings strongly suggest that the loss of spindle assembly checkpoint proteins, such as Bub1 and Mad2, may cause spontaneous miscarriages. PMID: 20643875
39. h-KNL1 functions to connect Bub1 and BubR1 with the hMis12, Ndc80, and Zwint-1 complexes. PMID: 21199919
40. findings show phosphorylation of histone H3-threonine 3 mediated by Haspin cooperates with Bub1-mediated histone 2A-serine 121 phosphorylation in targeting the chromosomal passenger complex to the inner centromere in fission yeast and human cells PMID: 20929775
41. the interaction between LANA and the kinetochore proteins CENP-F and Bub1 is important for KSHV genome tethering and its segregation to new daughter cells PMID: 20660191
42. Sequence alterations of mitotic checkpoint genes, hBUB1 and hBUBR1, were examined, and their gene transcripts were quantified using on-line, real-time quantitative reverse transcription-PCR in surgically resected human colorectal cancers PMID: 11782350
43. As part of a common complex in checkpoint-activated cells, Bub1 monitors different spindle events compared to BubR1 and integrates different signals into a single signal that is then relayed to the downstream cell cycle machinery. PMID: 11792804
44. Reduced expression and aberrant transcription of the hBUB1 gene were detected in acute myeloid leukemia specimens. PMID: 11999574
45. mutations in leukemia and lymphoma cells PMID: 12096343
46. Results suggest that mutation of the Bub1 gene might not play a role in human stomach and oral carcinogenesis. PMID: 12375025
47. mitotic checkpoint kinases BubR1 and BuB1, by binding to beta-adaptins, may play novel roles in the regulation of vesicular intracellular traffic PMID: 12419313
48. The checkpoint pathway can be abrogated by mutations in BUB1 in the pancreatic cancer cell line Hs766T. PMID: 12655561
49. No apparent selection trend was established with neoplastic progression or aneuploidy for BUB1 in Barrett esophagus. PMID: 15013707
50. Bub1 protects centromeric cohesion through Shugoshin in mitosis PMID: 15604152
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亞細胞定位：

Nucleus. Chromosome, centromere, kinetochore.
蛋白家族：

Protein kinase superfamily, Ser/Thr protein kinase family, BUB1 subfamily
組織特異性：

High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index.
數(shù)據(jù)庫鏈接：

HGNC: 1148

OMIM: 602452

KEGG: hsa:699

STRING: 9606.ENSP00000302530

UniGene: Hs.469649

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