Acetyl-Histone H2B type 1-B (K20) Recombinant Monoclonal Antibody

說明書

中文名稱：

乙酰-組蛋白 H2B type 1-B (K20)重組抗體
貨號：

CSB-RA010402A20acHU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in Hela whole cell lysate treated by 15mM sodium butyrate for 30min
All lanes Acetyl-Histone H2B type 1-B(K20)antibody at 0.135μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 15 KDa
Observed band size: 15 KDa

Immunocytochemistry analysis of CSB-RA010402A20acHU diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with CSB-RA010402A20acHU at 1:84,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Overlay histogram showing Hela cells stained with CSB-RA010402A20acHU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA010402A20acHU是針對組蛋白H2B第20位賴氨酸乙酰化修飾（Acetyl-Histone H2B type 1-B K20）開發的重組單克隆抗體。組蛋白H2B作為核小體核心成分，其K20位點的乙酰化修飾在表觀遺傳調控中發揮重要作用，參與染色質結構重塑、轉錄激活及DNA損傷修復等生物學過程。本產品經過ELISA、Western Blot（WB）、免疫細胞化學（ICC）、免疫熒光（IF）及流式細胞術（FC）等多平臺驗證，在WB實驗中表現出高特異性，推薦使用1:5000-1:10000稀釋比例可清晰檢測目標條帶；ICC/IF應用推薦1:50-1:500及1:30-1:200稀釋范圍，適用于細胞定位分析，能精準顯示核內乙酰化修飾信號；FC檢測則支持細胞群體表觀遺傳狀態分析。該抗體適用于染色質動態研究、組蛋白翻譯后修飾機制探索，以及發育生物學、腫瘤微環境等領域中表觀遺傳調控網絡的科研工作，為探索基因表達調控、干細胞多能性維持及疾病相關信號通路提供可靠工具。
Uniprot No.：

P33778
基因名：

HIST1H2BB
別名：

HIST1H2BB antibody; H2BFFHistone H2B type 1-B antibody; Histone H2B.1 antibody; Histone H2B.f antibody; H2B/f antibody
反應種屬：

Human
免疫原：

A synthesized peptide
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

21F11
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, ICC, IF, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:5000-1:10000
ICC	1:50-1:500
IF	1:30-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
基因功能參考文獻：
1. Functional crosstalk between histone H2B ubiquitylation and H2A modifications and variants has been uncovered. PMID: 29643390
2. RNF20 and H2Bub1 promotes chronic colonic inflammation and inflammation-associated colorectal cancer in mice and humans, partly by augmenting NF-kappaB activity and attenuating the antitumoral T cell response. PMID: 26854224
3. RNF20-mediated H2B ubiquitination at DSBs plays a critical role in HRR through chromatin remodeling PMID: 21362548
亞細胞定位：

Nucleus. Chromosome.
蛋白家族：

Histone H2B family
數據庫鏈接：

HGNC: 4751

OMIM: 602803

KEGG: hsa:3018

STRING: 9606.ENSP00000350580

UniGene: Hs.553494

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