Acetyl-Histone H2A type 1-B/E (K9) Recombinant Monoclonal Antibody
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中文名稱：乙酰-組蛋白 H2A type 1-B/E (K9)重組抗體
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貨號：CSB-RA010385A09acHU
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規格：￥1320
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圖片：
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Immunocytochemistry analysis of CSB-RA010385A09acHU diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with CSB-RA010385A09acHU at 1:56,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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其他：
產品詳情
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產品描述：CSB-RA010385A09acHU重組單克隆抗體特異性識別組蛋白H2A亞型1-B/E第9位賴氨酸乙酰化修飾（Acetyl-Histone H2A type 1-B/E K9），該表觀遺傳標記在染色質重塑和基因轉錄調控中發揮重要作用。本抗體通過重組表達技術制備，具有高批次一致性和優異特異性，經ELISA、免疫細胞化學（ICC）及免疫熒光（IF）等多平臺驗證，推薦使用濃度分別為ICC 1:50-1:500和IF 1:30-1:200。實驗數據顯示其能精準識別內源性乙酰化H2A蛋白，適用于探究細胞分化、DNA損傷修復及表觀遺傳調控機制等研究領域。在染色質動態分析、干細胞重編程模型及表觀遺傳藥物篩選等科研場景中表現卓越，為研究組蛋白修飾與基因表達調控網絡提供可靠工具。
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Uniprot No.：
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基因名：HIST1H2AB
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別名：H2a 615 antibody; H2A antibody; H2A GL101 antibody; H2A histone family member A antibody; H2A.1 antibody; H2A.2 antibody; H2A/a antibody; H2A/m antibody; H2A/O antibody; H2A/q antibody; H2A1B_HUMAN antibody; H2AFA antibody; H2AFE antibody; H2AFL antibody; H2AFM antibody; H2AFO antibody; H2AFQ antibody; HIST1H2AE antibody; HIST1H2AJ antibody; HIST2H2AA antibody; HIST2H2AA3 antibody; HIST2H2AB antibody; HIST2H2AC antibody; Histone 1 H2ae antibody; Histone 2 H2aa3 antibody; Histone 2 H2ab antibody; Histone 2 H2ac antibody; Histone H2A type 1 B antibody; Histone H2A type 1 C antibody; Histone H2A type 1 E antibody; Histone H2A type 1 J antibody; Histone H2A type 1-B/E antibody; Histone H2A.2 antibody; Histone H2A/a antibody; Histone H2A/m antibody; MGC74460 antibody
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反應種屬：Human
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免疫原：A synthesized peptide
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：3H5
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, ICC, IF
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推薦稀釋比：
Application Recommended Dilution ICC 1:50-1:500 IF 1:30-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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靶點詳情
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功能：Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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亞細胞定位：Nucleus. Chromosome.
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蛋白家族：Histone H2A family
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數據庫鏈接：
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