ZBTB33 Recombinant Monoclonal Antibody

說明書

中文名稱：

ZBTB33 Recombinant Monoclonal Antibody
貨號：

CSB-RA956726A0HU
規格：

￥1320
圖片：
Flow cytometric analysis of ZBTB33 expression in HepG2 cells using ZBTB33 antibody. Green, isotype control; red, ZBTB33.

Immunocytochemical staining of HepG2 cells with ZBTB33 antibody. Nuclei were stained blue with DAPI; ZBTB33 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using ZBTB33 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ZBTB33 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

Q86T24
基因名：

ZBTB33
別名：

ZBTB33; Zinc Finger And BTB Domain Containing 33; ZNF348; WUGSC:H_DJ525N14.1; ZNF-Kaiso; Zinc Finger And BTB Domain-Containing Protein 33; Transcriptional Regulator Kaiso; Kaiso; Kaiso Transcription Factor; KAISO
反應種屬：

Human, Rat
免疫原：

Recombinant Human ZBTB33 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

24C1
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC, ICC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. Represses expression of MMP7 in conjunction with transcriptional corepressors CBFA2T3, CBFA2T2 and RUNX1T1.
基因功能參考文獻：
1. The level of miR-4262 was found to be significantly decreased in CC tissues and cell lines. Moreover, decreased expression of miR-4262 was closely related to increased expression of Kaiso (ZBTB33). PMID: 28800784
2. extracellular microenvironment signals regulate RhoH and Kaiso to modulate actin-cytoskeleton structure and transcriptional activity during T cell migration PMID: 27574848
3. Kaiso silencing had similar effects as miR-181a overexpression in glioma cells, whereas overexpression of Kaiso in glioma cells partially reversed the inhibitory effects of the miR-181a mimic. PMID: 30036882
4. data show that Kaiso favors an mCpG over a CpG site by 2 orders of magnitude in affinity and that an important component of this effect is the presence of hydrophobic and CH...O contacts involving E535. PMID: 29546986
5. High levels of nuclear Kaiso expression were detected in patients with a higher degree of African heritage compared to their Caucasian counterparts, suggesting a role for Kaiso in triple-negative breast cancer racial disparity. PMID: 28887687
6. Kaiso promotes Jagged-1 expression, which may have implications in Notch-mediated colon cancer progression PMID: 28637464
7. A role for Kaiso in the proliferation. PMID: 28333150
8. the pathological features of the metastatic activity of parental (Kaisopositive) versus Kaisodepleted MDA-231 cells, are reported. PMID: 28880889
9. Suggest a role for Kaiso in the progression of pancreatic ductal adenocarcinomas, involving the epithelial mesenchymal transformation markers, E-cadherin and Zeb1. PMID: 27424525
10. a novel mechanism by which ZBTB33 mediates the cyclin D1/cyclin E1/RB1/E2F pathway, controlling passage through the G1 restriction point and accelerating cancer cell proliferation. PMID: 27694442
11. GR is a putative target gene of Kaiso. PMID: 26424557
12. Kaiso high expression correlated with invasion of prostate cancer. PMID: 26734997
13. Results show that Kaiso binding to unmethylated Kaiso binding site in the human ICR1 is necessary for ICR1 methylation maintenance and affects transcription rates of the lncRNA H19. PMID: 27152123
14. Kaiso modulates HIF1A gene expression by binding to the methylated HIF1A promoter in a region proximal to the autoregulatory HIF-1 binding site, primarily during hypoxia. PMID: 26514431
15. NF-kappaB response element, located close to the p53RE#1, mediates APAF1 transcriptional repression by affecting interaction between KAISO and p53 PMID: 26183023
16. KAISO is a regulator of p53-mediated transcription of CDKN1A and apoptotic genes PMID: 25288747
17. Data indicate that Kaiso protein participates in the regulation of beta-catenin mRNA expression by interacting with p120-catenin in the lung cancer cell lines. PMID: 24498333
18. Nuclear localization of Kaiso promotes the poorly differentiated phenotype and EMT in infiltrating ductal carcinomas. PMID: 24570268
19. The optimized knockdown with p120 and Kaiso siRNAs further expands the size of HCEC monolayers without endothelial mesenchymal transition (EMT) via selective activation of p120/Kaiso signaling that requires the RhoA-ROCK-noncanonical BMP-NFkB signaling. PMID: 24474278
20. Transcription factor Kaiso does not interact with hydroxymethylated DNA within CTGCNA sequence context PMID: 23888785
21. presence of MTG16 in this complex, and its contributions to transcriptional repression both required Kaiso binding to its binding site on DNA, establishing MTG16-Kaiso binding as functionally relevant in Kaiso-dependent transcriptional repression PMID: 23251453
22. Kaiso represses the cell cycle gene cyclin D1 via sequence-specific and methyl-CpG-dependent mechanism. PMID: 23226276
23. These findings establish a defined oncogenic role of Kaiso in promoting the progression of prostate cancer. PMID: 22974583
24. High cytoplasmic kaiso expression is associated with thymoma. PMID: 22833212
25. nuclear Kaiso is common in clinically aggressive ductal breast cancer, while cytoplasmic Kaiso and a p120-mediated relief of Kaiso-dependent transcriptional repression characterize invasive lobular carcinoma (ILC). PMID: 22662240
26. Molecular basis for recognition of methylated and specific DNA sequences by the zinc finger protein Kaiso PMID: 22949637
27. Selective activation of p120ctn-Kaiso signaling to unlock contact inhibition of ARPE-19 cells without epithelial-mesenchymal transition. PMID: 22590627
28. Data show that Kaiso requires all three zinc fingers plus adjacent protein regions for DNA recognition. PMID: 22300642
29. Results suggest that p120ctn isoforms 1 and 3 up-regulate cyclin D1, and thereby cyclin E, resulting in the promotion of cell proliferation and cell cycle progression in lung cancer cells. PMID: 22276175
30. when released from E-cadherin by Wnt3a-stimulated phosphorylation, p120-catenin controls the activity of Kaiso, enhancing its binding to repressed promoters and relieving its inhibition of the beta-catenin-Tcf-4 transcriptional complex. PMID: 21670201
31. Increased delta-catenin expression is critical for maintenance of the malignant phenotype of lung cancer, making delta-catenin a candidate target protein for future cancer therapeutics. PMID: 21070476
32. In endothelial cells, p120ctn has a transcription repression function through regulation of Kaiso, possibly as a cofactor with the transcription factor. PMID: 20382170
33. Data strongly imply that Kaiso's function as a transcriptional regulator might be linked to the control of the cell cycle and to cell proliferation in cancer. PMID: 20169156
34. Bifunctional role of domain zinc fingers of methyl-DNA-binding protein Kaiso PMID: 20586187
35. differential expression of p120(ctn) and Kaiso mRNA was observed in human coronary artery endothelial cells depending on how laminar shear stress was applied in relation to the wounding process PMID: 14699141
36. Kaiso translocates to the nucleus to regulate transcription of genes with diverse roles in cell growth and development PMID: 15564377
37. Data imply an unexpected influence of the microenvironment on Kaiso expression and localizationin colorectal adenocarcinoma. PMID: 15781635
38. Kaiso associates with the matrilysin promoter in vivo. PMID: 15817151
39. KAISO binds specifically to the methylated, but not the unmethylated, sequence in the first exon of the tyrosine hydroxylase [TH]gene and thus may play an important role in modulating human TH gene expresison. PMID: 15953356
40. the Kaiso-CTCF interaction negatively regulates CTCF insulator activity PMID: 16230345
41. Our study suggests that this gene is not implicated in the RTT molecular pathogenesis PMID: 16530985
42. p120 and Kaiso regulate Helicobacter pylori-induced expression of matrix metalloproteinase-7 PMID: 18653469
43. Kaiso represses methylated tumor suppressor genes and can bind in a methylation-dependent manner to the CDKN2A in human colon cancer cell lines. Kaiso depletion induced tumor suppressor gene expression without affecting DNA methylation levels. PMID: 18794111
44. Kaiso plays some specific role in centrosome assembly and function, which is not restricted to mitosis PMID: 19502788
45. Data suggest cytoplasmic Kaiso expression is associated with poor prognosis of NSCLC and various subcellular localizations of Kaiso may play differential biological roles in NSCLC. PMID: 19508730
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亞細胞定位：

Nucleus. Cytoplasm. Note=Also cytoplasmic in cells grown at high densities.
組織特異性：

Expressed in vascular endothelium.
數據庫鏈接：

HGNC: 16682

OMIM: 300329

KEGG: hsa:10009

STRING: 9606.ENSP00000314153

UniGene: Hs.143604

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