WARS1 Recombinant Monoclonal Antibody
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中文名稱：WARS1 Recombinant Monoclonal Antibody
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貨號：CSB-RA293319A0HU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in: A-431 whole cell lysate(30μg), HeLa whole cell lysate, HL-60 whole cell lysate(30μg)
All lanes: WARS1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 3min -
Overlay Peak curve showing HepG2 cells stained with CSB-RA293319A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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Uniprot No.：
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基因名：
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別名：Gamma 2 antibody; GAMMA-2 antibody; Gamma2 antibody; hWRS antibody; IFI 53 antibody; IFI53 antibody; IFP 53 antibody; IFP53 antibody; Interferon induced protein 53 antibody; Interferon-induced protein 53 antibody; SYWC_HUMAN antibody; T2-TrpRS antibody; TrpRS antibody; Tryptophan tRNA ligase; cytoplasmic antibody; Tryptophan tRNA ligase 1 cytoplasmic antibody; Tryptophan tRNA ligase antibody; Tryptophan--tRNA ligase antibody; Tryptophanyl tRNA synthetase antibody; Tryptophanyl tRNA synthetase cytoplasmic antibody; WARS antibody; WARS protein antibody; WRS antibody
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反應種屬：Human
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免疫原：A synthesized peptide derived from human WARS1
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：8D4
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.
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基因功能參考文獻：
- Based on these results, secretion of full-length tryptophanyl-tRNA synthetase appears to work as a primary defence system against infection, acting before full activation of innate immunity. PMID: 27748732
- findings establish WARS as a gene whose mutations may cause distal hereditary motor neuropathy and alter canonical and non-canonical functions of tryptophanyl-tRNA synthetase. PMID: 28369220
- Overexpression of WARS predicts no recurrence and good survival for triple-negative breast cancer patients. PMID: 26209610
- Tryptophanyl-tRNA synthetase expression is up-regulated in patients with rheumatoid arthritis. PMID: 24515434
- Genes within recently identified loci associated with waist-hip ratio (WHR) exhibit fat depot-specific mRNA expression, which correlates with obesity-related traits. Adipose tissue (AT) mRNA expression of 6 genes (TBX15/WARS2, STAB1, PIGC, ZNRF3, GRB14) PMID: 23670221
- Indoleamine2,3-dioxygenase and tryptophanyl-tRNA synthetase may play critical roles in the immune pathogenesis of chronic kidney disease. PMID: 23651343
- Naturally occurring fragments of the two proteins involved in translation, TyrRS and TrpRS, have opposing activities on angiogenesis. PMID: 21442253
- Tryptophanyl-tRNA synthetase down-regulation by hypoxia may be a factor responsible for low TrpRS in pancreatic tumors with high metastatic ability. PMID: 21926542
- Mini-tryptophanyl-tRNA synthetase inhibited ischemic angiogenesis in rats. PMID: 20963594
- Tryptophanyl-tRNA synthetase is a multidomain protein exhibiting excellent allosteric communication, and this research has provided valuable structural as well as functional insights into the protein. PMID: 19768679
- Low tryptophanyl-tRNA synthetase is associated with recurrence in colorectal cancer. PMID: 19900940
- In this study, we show that a recombinant form of a COOH-terminal fragment of TrpRS is a potent antagonist of vascular endothelial growth factor-induced angiogenesis in a mouse model and of naturally occurring retinal angiogenesis in the neonatal mouse PMID: 11773625
- Thus, protein synthesis may be linked to the regulation of angiogenesis by a natural fragment of TrpRS PMID: 11773626
- Recognition by tryptophanyl-tRNA synthetases of discriminator base on tRNATrp from three biological domains PMID: 11834741
- The recently discovered antiangiogenic and cell-signaling activities of tryptophanyl-tRNA synthetase bioactive fragments are discussed in this review. PMID: 12416978
- TrpRS may have a role in the maintenance of vascular homeostasis PMID: 14630953
- results suggest that mammalian and bacterial tryptophanyl-tRNA synthetase might use different mechanisms to recognize the substrate and modeling studies indicate that transfer RNA binds with the dimeric enzyme PMID: 14660560
- A crystal structure of human tryptophanyl-tRNA synthetase was solved at 2.1 A with a tryptophanyl-adenylate bound at the active site PMID: 14671330
- May play an important role in the intracellular regulation of protein synthesis under conditions of oxidative stress. PMID: 15628863
- These crystals captured two conformations of the human tryptophanyl-tRNA synthetase and tRNATrp complex, which are nearly identical with respect to the protein and a bound tryptophan. PMID: 16724112
- The first crystal structure of human tryptophanyl-tRNA synthetase (hTrpRS) in complex with tRNA(Trp) and Trp which, together with biochemical data, reveals the molecular basis of a novel tRNA binding and recognition mechanism. PMID: 16798914
- Results provide the first evidence of the involvement of heme in regulation of TrpRS aminoacylation activity. PMID: 17877375
- the annexin II-S100A10 complex, which regulates exocytosis, forms a ternary complex with TrpRS. PMID: 17999956
- Analysis of the molecular basis of the mechanisms of the substrate recognition and the activation reaction by tryptophanyl-tRNA synthetase. PMID: 18180246
- Indoleamine 2,3-dioxygenase (IDO)-expression in antigen-presenting cells (APCs) may control autoimmune responses by depleting the available tryptophan, whereas tryptophanyl-tRNA synthetase (TTS) may counteract this effect PMID: 19363598
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亞細胞定位：Cytoplasm.
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蛋白家族：Class-I aminoacyl-tRNA synthetase family
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數據庫鏈接：
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