USP14 Recombinant Monoclonal Antibody

說明書

中文名稱：

USP14重組抗體
貨號：

CSB-RA587609A0HU
規(guī)格：

￥1320
圖片：
Western Blot
Positive WB detected in: PC3 whole cell lysate, Ntera-2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, U251 whole cell lysate
All lanes: USP14 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 57, 53, 55 kDa
Observed band size: 55-72 kDa

Immunofluorescence staining of PC-3 cell with CSB-RA587609A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 517-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

USP14重組單克隆抗體（CSB-RA587609A0HU）是一種高特異性抗體，靶向泛素特異性蛋白酶14（USP14）。USP14作為去泛素化酶家族成員，通過調(diào)控蛋白酶體介導(dǎo)的蛋白質(zhì)降解通路參與細(xì)胞周期、應(yīng)激反應(yīng)及神經(jīng)系統(tǒng)疾病相關(guān)機(jī)制研究。本產(chǎn)品經(jīng)ELISA、蛋白質(zhì)印跡（WB）和免疫熒光（IF）多平臺驗(yàn)證，展現(xiàn)出優(yōu)異的靈敏度和特異性。在WB實(shí)驗(yàn)中，推薦稀釋比例為1:500至1:2000，適用于多種樣本類型的靶蛋白檢測；IF實(shí)驗(yàn)推薦1:50至1:200稀釋度，可清晰定位細(xì)胞內(nèi)USP14的亞細(xì)胞分布。該抗體適用于研究蛋白質(zhì)穩(wěn)態(tài)失衡相關(guān)疾病模型（如神經(jīng)退行性疾病）、腫瘤發(fā)生機(jī)制探索以及藥物靶點(diǎn)篩選等科研領(lǐng)域。其穩(wěn)定的批次間一致性和低交叉反應(yīng)性，為定量分析、蛋白互作研究及功能調(diào)控實(shí)驗(yàn)提供了可靠工具，尤其適用于需要高分辨率成像或低豐度蛋白檢測的實(shí)驗(yàn)設(shè)計(jì)。
Uniprot No.：

P54578
基因名：

USP14
別名：

Ubiquitin carboxyl-terminal hydrolase 14 (EC 3.4.19.12) (Deubiquitinating enzyme 14) (Ubiquitin thioesterase 14) (Ubiquitin-specific-processing protease 14), USP14, TGT
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from human USP14
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

28C4
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點(diǎn)詳情

功能：

Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation.
基因功能參考文獻(xiàn)：
1. By displacing USP14, TRIM11 changes proteasome composition and suppresses both catalytic and non-catalytic effects of USP14 PMID: 29581427
2. Usp14 and Ube3c cycle together on and off proteasomes, and the presence of ubiquitinated substrates promotes their association. This mechanism enables proteasome activity to adapt to the supply of substrates. PMID: 28396413
3. Study detected elevated USP14 expression at both mRNA and protein levels, and was significantly associated with distant metastasis. PMID: 28509417
4. These findings suggested that USP14 induces NF-kappaB activity and ERK1/2 phosphorylation triggered by microbial infection. PMID: 28364380
5. Ubiquitin-specific protease 14 regulates cell proliferation and apoptosis in oral squamous cell carcinoma PMID: 27592452
6. our report demonstrates significant value in targeting USP14/UCHL5 with VLX1570 in drug-resistant Waldenstrom macroglobulinemia (WM) and carries a high potential for clinical translation PMID: 27813535
7. USP14 promotes prostate cancer progression. PMID: 28151478
8. without a ubiquitinated substrate present, Usp14 suppresses multiple proteasomal activities, especially basal ATP consumption and degradation of non-ubiquitinated proteins. These allosteric effects thus reduce ATP hydrolysis by inactive proteasomes and nonspecific proteolysis and enhance proteasomal specificity for ubiquitinated proteins. PMID: 28416611
9. USP14 regulates autophagy by negatively controlling K63 ubiquitination of Beclin 1 PMID: 27542828
10. USP14 plays an important role in the progression and metastasis of esophageal squamous cell carcinoma. PMID: 27629392
11. USP14 participates in CAM-DR of MM through acting as a bridge between Bcl-xl apoptotic pathway and Wnt-signaling pathways and may be represented as a good candidate for pursuing clinical trials in MM. PMID: 26710889
12. Results show that USP14 mRNA is overexpressed in hepatocellular carcinoma (HCC) tissues and inversely correlates with miR-4782-3p level which binds to USP14 3'UTR to regulate its expression. PMID: 26782643
13. Results found USP14 amplification and overexpression in many different cancers. Its overexpression in low-expression cell lines promoted cell proliferation and migration, whereas its downregulation suppressed tumor cell proliferation, increased apoptosis rate, and decreased cell migration and invasion suggesting an oncogenic role in various types of cancer. PMID: 26938858
14. Increased USP14 expression in patients with breast cancer was associated with a poorer prognosis. PMID: 26712154
15. Akt-mediated phosphorylation of USP14 at Ser432, which normally blocks its catalytic site in the inactive conformation, activates its deubiquitinating activity in vitro and in cells. PMID: 26523394
16. our findings suggested that USP14 is involved in the progression of hepatocellular carcinoma(HCC) and may be a useful therapeutic target in HCC PMID: 26397990
17. Cell surface ubiquitination precedes endocytosis, after which USP14 acts as an ubiquitin-binding protein that targets the ubiquitinated GABA B receptor to lysosomal degradation and promotes its deubiquitination. PMID: 26817839
18. USP14 shows a marked preference for ubiquitin-cyclin B conjugates that carry more than one ubiquitin modification or chain PMID: 27074503
19. our findings suggest that USP14 is involved in the progression of epithelial ovarian cancer PMID: 25429837
20. Downregulation of USP14 expression arrested the cell cycle, which may be related to beta-catenin degradation. PMID: 23702845
21. b-AP15 is an inhibitor of deubiquitylating enzyme USP14 and UCHL5 induces apoptosis in multiple myeloma and overcomes bortezomib resistance. PMID: 24319254
22. overexpression of HA-USP14 increased the LPS-, TNFalpha-, or Escherichia coli-induced IL-8 release in human lung epithelial cells. PMID: 23615914
23. the decrease in proteolysis of proteasomal substrates during aging is independent of the increased USP14 activity and that the reciprocal regulation of USP14 and proteasomal catalytic activity may be necessary to maintain cellular ubiquitin homeostasis. PMID: 23291607
24. High Ubiquitin-specific protease 14 expression associated with intrahepatic cholangiocarcinoma cell differentiation. PMID: 21627382
25. the catalytic cleft leading to the active site of USP14 is blocked by two surface loops. Binding by ubiquitin induces a significant conformational change thereby allowing access of the ubiquitin C-terminus to the active site. PMID: 16211010
26. Results show the overall survival rate was worse in patients with a high USP14/TGT60 kD expression level and suggest that USP14/TGT60 kD also controls the fate of proteins that regulate tumor invasion and metastasis. PMID: 16465409
27. Neuronal expression of full-length USP14 is able to restore the levels of monomeric ubiquitin in the brains of transgenic ataxia mice. PMID: 17079671
28. USP14 were over-expressed in ovarian serous cystadenocarcinoma tissues, suggesting that the activity of ubiquitin-proteasome system is obviously enhanced in ovarian cancer. PMID: 17553343
29. These findings suggest that USP14 is a novel player in the unfolded protein response by serving as a physiological inhibitor of ER-associated degradation under the non-stressed condition. PMID: 19135427
30. Data from transgenic mice define a critical role for Usp14 at mammalian synapses and suggest a requirement for local ubiquitin recycling by the proteasome to control the development and function of neuromuscular junctions. PMID: 19726649
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亞細(xì)胞定位：

Cytoplasm. Cell membrane; Peripheral membrane protein.
蛋白家族：

Peptidase C19 family, USP14/UBP6 subfamily
數(shù)據(jù)庫鏈接：

HGNC: 12612

OMIM: 607274

KEGG: hsa:9097

STRING: 9606.ENSP00000261601

UniGene: Hs.464416

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