STAU1 Recombinant Monoclonal Antibody
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中文名稱：STAU1 Recombinant Monoclonal Antibody
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貨號：CSB-RA193685A0HU
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規格：￥1320
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圖片：
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Flow cytometric analysis of STAU1 expression in HT-1080 cells using STAU1 antibody. Green, isotype control; red, STAU1. -
Immunocytochemical staining of HT-1080 cells with STAU1 antibody. Nuclei were stained blue with DAPI; STAU1 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. -
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using STAU1 antibody. Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm. -
Western blotting analysis using STAU1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STAU1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
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其他：
產品詳情
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Uniprot No.：
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基因名：STAU1
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別名：STAU1; Staufen Double-Stranded RNA Binding Protein 1; PPP1R150; STAU; Double-Stranded RNA-Binding Protein Staufen Homolog 1; Protein Phosphatase 1, Regulatory Subunit 150; Staufen, RNA Binding Protein, Homolog 1 (Drosophila); Staufen (Drosophila, RNA-Binding Protein); Staufen, RNA Binding Protein (Drosophila); Staufen, RNA Binding Protein, Homolog 1
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反應種屬：Human, Mouse, Rat
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免疫原：Recombinant Human STAU1 protein
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：20A2
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, FC, ICC, IHC
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推薦稀釋比：
Application Recommended Dilution WB 1:1000-1:5000 FC 1:200-1:2000 ICC 1:100-1:1000 IHC 1:100-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Binds double-stranded RNA (regardless of the sequence) and tubulin. May play a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site.; (Microbial infection) Plays a role in virus particles production of many viruses including of HIV-1, HERV-K, ebola virus and influenza virus. Acts by interacting with various viral proteins involved in particle budding process.
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基因功能參考文獻：
- recruited to mutant ATXN2 aggregates in spinocerebellar ataxia type 2 fibroblasts PMID: 30194296
- Staufen1 has differential roles in embryonal versus alveolar rhabdomyosarcoma through the control of proliferative and apoptotic pathways, respectively. PMID: 28211476
- Mechanistically, we suggest that SNHG5 stabilizes the target transcripts by blocking their degradation by STAU1. Accordingly, depletion of STAU1 rescues the apoptosis induced after SNHG5 knockdown. Hence, we characterize SNHG5 as a lncRNA promoting tumour cell survival in colorectal cancer. PMID: 28004750
- E2F1 induces TINCR transcriptional activity and accelerates gastric cancer progression via activation of TINCR/STAU1/CDKN2B signaling axis. PMID: 28569791
- ADAR1p110 isoform competitively inhibits binding of Staufen1 to the 3'-untranslated-region dsRNAs and antagonizes Staufen1-mediated mRNA decay. PMID: 28436945
- Stau1 is a stress response gene that remains efficiently translated during hypoxia and ER stress despite the substantial global inhibition of cap-dependent protein translation, promoting cell recovery following stress PMID: 27644878
- Our findings suggest that HCV may appropriate Stau1 to its advantage to prevent PKR-mediated inhibition of eIF2alpha, which is required for the synthesis of HCV proteins for translocation of viral RNA genome to the polysomes for efficient translation and replication. PMID: 27106056
- together, these data highlight the broad impact of Stau1 as a splicing regulator and suggest that Stau1 may act as a disease modifier in DM1. PMID: 26824521
- expression of human Staufen1 is essential for proper dendritic arborisation during neuroblastoma cell differentiation, yet it is not necessary for maintenance of differentiated state, and suggest potential human Stauf1 mRNA targets involved in the process PMID: 25423178
- The changing pattern of STAU distribution during meiotic maturation of human oocytes implicates a novel mechanism for the regulation of protein synthesis based on mRNA localization PMID: 25620022
- in vivo atlas of mRNA secondary structures recognized by Staufen 1 PMID: 25799984
- TINCR, together with Staufen1, seems to stabilize a subset of mRNAs required for epidermal differentiation. PMID: 24019000
- A common sequence signature consisting of two opposite-polarity Alu motifs was present in the hStau1-associated mRNAs and was shown to be sufficient for binding to hStau1 and hStau1-dependent stimulation of protein expression. PMID: 24470147
- Staufen1 is an important factor in HCV replication and it might play a role early in the HCV replication cycle, rather than virion morphogenesis. PMID: 23907398
- STAU1 binding to a 3'-UTR SBS was previously shown to trigger STAU1-mediated mRNA decay (SMD) by directly recruiting the ATP-dependent RNA helicase UPF1 PMID: 24056942
- These results suggest a new role for Staufen-1 as a cellular Rec and human endogenous retrovirus family HERV-K Gag cofactor. PMID: 23926355
- this study reports the identification of two new hGIP-interacting partners, DTX1 and STAU1. PMID: 23395680
- The regulation of STAU1 mediated mRNA decay efficiency by NS1 may contribute to building a more favorable cellular environment for viral replication. PMID: 23722113
- STAU1 binding to 3' UTR IRAlus functions along with 3' UTR IRAlus-mediated nuclear retention to suppress the shutdown of cellular translation triggered by PKR binding to endogenous cytoplasmic dsRNAs PMID: 23824540
- SSM-mediated hSTAU1 dimerization increases the efficiency of SMD by augmenting hSTAU1 binding to the ATP-dependent RNA helicase hUPF1 PMID: 23524536
- TDP-43 is physically associated with fragile X mental retardation protein (FMRP) and Staufen (STAU1) to form a functional complex. PMID: 22584570
- For Stau1-mediated mRNA decay, Stau1 binds to the 3' untranslated region of target mRNA and recruits Upf1 to elicit rapid mRNA degradation. PMID: 22503102
- our results unravel a novel function for Staufen1 in splicing regulation and indicate that it may positively modulate the complex DM1 phenotype PMID: 22431750
- the specific region responsible for the interaction between Stau1 and influenza A Virus NS1 was identified. PMID: 21945618
- findings that the binding of STAU1 to mRNAs can be transactivated by long non-coding RNAs uncovers an unexpected strategy that cells use to recruit proteins to mRNAs and mediate the decay of these mRNAs PMID: 21307942
- Gag specifically recruited Staufen1 to the detergent insoluble membranes supporting a key function for this host factor during virus assembly. PMID: 20459747
- These results indicate a role for hStau1 in late events of the influenza virus infection, possibly during virus morphogenesis. PMID: 20504931
- Work provides new evidence that Staufen1-containing HIV-1 RNPs preferentially form over other cellular silencing foci and are involved in assembly, localization and encapsidation of vRNA. PMID: 20053637
- The oligomeric composition of Stau1-containing complexes and the presence of specific mRNAs have been confirmed by biochemical approaches involving two successive immunoprecipitations of Stau1-tagged molecules followed by qRT-PCR amplification. PMID: 20075165
- Data show that the Staufen isoform Stau(55)co-fractionates with ribosomes, suggesting that it plays some role in translation. PMID: 12133005
- These data show that Staufen, pr55(Gag), and genomic RNA are part of the same intracellular complex and support a role for Staufen in pr55(Gag) function in viral assembly, genomic RNA encapsidation, and the generation of infectious viral particles. PMID: 15024055
- The identity of proteins present in staufen complexes from RNA granules indicates that staufen has a role in transport and translation of messenger RNAs. PMID: 15121898
- Stau1 influences HIV-1 assembly by modulating pr55Gag-pr55Gag interactions, as shown in a live cell interaction assay PMID: 17428849
- Stau1 influences the expression of a wide variety of physiologic transcripts and metabolic pathways. PMID: 17510634
- Stau1- and Stau2-mRNPs associate with distinct but overlapping sets of cellular mRNAs. PMID: 18094122
- 12 amino acids at the N-terminal end of Stau1 is necessary to increase Pr55Gag multimerization and particle release. PMID: 18498651
- isolated a novel transcript of STAU1, coding a novel isoform that has six amino acids more (SFPLKQ) than isoform a PMID: 18806890
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亞細胞定位：Cytoplasm. Rough endoplasmic reticulum. Note=Localizes exclusively with the rough reticulum endoplasmic (RER).
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組織特異性：Widely expressed. Expressed in brain, pancreas, heart, skeletal muscles, liver, lung, kidney and placenta.
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