SOCS7 Recombinant Monoclonal Antibody
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中文名稱：SOCS7重組抗體
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貨號：CSB-RA594821A0HU
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規格：￥1320
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圖片：
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IHC image of CSB-RA594821A0HU diluted at 1:50 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.43% DAB. -
Overlay Peak curve showing HepG2 cells stained with CSB-RA594821A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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產品描述：CSB-RA594821A0HU 重組單克隆抗體是針對SOCS7蛋白開發的高特異性科研工具。SOCS7作為細胞因子信號抑制因子家族成員，通過調控JAK-STAT信號通路參與免疫應答、細胞周期調控及代謝平衡等生物學過程。本抗體經ELISA、免疫組化（IHC）和流式細胞術（FC）多平臺驗證，在推薦稀釋比例（IHC 1:50-1:200，FC 1:50-1:200）下展現出優異的目標蛋白結合能力，可精準識別天然構象及變性狀態的SOCS7，實驗結果具有高重復性與低背景干擾特性。其應用涵蓋體外細胞模型、組織切片及原代細胞分析，適用于探究SOCS7在炎癥反應、腫瘤微環境調控或代謝性疾病中的分子機制研究，為信號轉導通路解析、蛋白質相互作用分析及疾病相關生物標記物檢測提供可靠工具。本產品嚴格遵循質量控制標準，適用于基礎科研領域的蛋白表達定位及功能研究。
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Uniprot No.：
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基因名：
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別名：Suppressor of cytokine signaling 7 (SOCS-7) (Nck, Ash and phospholipase C gamma-binding protein) (Nck-associated protein 4) (NAP-4), SOCS7, NAP4 SOCS6
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反應種屬：Human
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免疫原：A synthesized peptide derived from Human SOCS7
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：23E4
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, IHC, FC
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推薦稀釋比：
Application Recommended Dilution IHC 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Regulates signaling cascades probably through protein ubiquitination and/or sequestration. Functions in insulin signaling and glucose homeostasis through IRS1 ubiquitination and subsequent proteasomal degradation. Inhibits also prolactin, growth hormone and leptin signaling by preventing STAT3 and STAT5 activation, sequestering them in the cytoplasm and reducing their binding to DNA. May be a substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
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基因功能參考文獻：
- This study showed that increased protein levels of SOCS-4 and SOCS-7 in Alzheimer's disease brains. PMID: 25286386
- This SOCS7 knockdown-attributed effect could be due to a precise anti-PLCg-1 role. PMID: 25162020
- genetic variants in the SOCS7 gene do not impact variation in glucose homeostasis traits and only minimally impact risk of T2DM in the Old Order Amish PMID: 23767996
- Insulin Like Growth Factor I treatment and SOCS7 loss have synergistically resulted in increased growth and migration of MCF7 and in increased migration of MDA-MB-231 cells. PMID: 24046004
- The present report describes for the first time associations between common variants in SOCS7 gene and obesity, central obesity, insulin resistance and disorders of lipid metabolism in nondiabetic men from Argentina. PMID: 22397880
- SOCS7 functioned as an oncogene, the finding that revealed a novel mechanism of carcinogenesis in bladder cancer cells. PMID: 23392170
- LNCaP-S17 cells are resistant to exogenous IL-6-induced neuroendocrine differentiation due to increased levels of CIS/SOCS7 that block activation of JAK2-STAT3 pathways. PMID: 22213096
- Higher mRNA expression levels of SOCS1, 3, 4 and 7 are significantly associated with earlier tumour stage and better clinical outcome in human breast cancer. PMID: 20433750
- findings show that PPAR-gamma is involved in the regulation of SOCS-7 expression by hepatitis C virus core protein genotype 3a PMID: 20357037
- Taken together, our data indicate that SOCS-7 interacts with vinexin and the actin cytoskeleton. PMID: 15242778
- Results indicate that SOCS-7 is a dysregulator of prolactin, leptin, and growth hormone signaling and that its mode of action is a variation of SOCS protein inhibition of cytokine-inducible STAT3 and 5-mediated signal transduction. PMID: 15677474
- data suggest that SOCS7 is a potent regulator of glucose homeostasis and insulin signaling PMID: 16127460
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亞細胞定位：Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus. Note=Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Rapidly relocalizes to the nucleus after UV irradiation. Cytoplasmic location depends upon SEPT7 presence.
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組織特異性：Expressed in brain and leukocytes. Also in fetal lung fibroblasts and fetal brain.
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數據庫鏈接：
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