RPL13 Recombinant Monoclonal Antibody
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中文名稱：RPL13 Recombinant Monoclonal Antibody
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貨號：CSB-RA595981A0HU
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規(guī)格：￥1320
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圖片：
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Western Blot
Positive WB detected in: MCF-7 whole cell lysate(30μg), HEK293T whole cell lysate(30μg), HepG2 whole cell lysate(30μg), Hela whole cell lysate(30μg), Jurkat whole cell lysate(30μg), HT-29 whole cell lysate(30μg), Rat liver tissue lysate(30μg)
All lanes:RPL13 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 24 kDa
Observed band size: 28 kDa
Exposure time：30s -
IHC image of CSB-RA595981A0HU diluted at 1:100 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
IHC image of CSB-RA595981A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
Immunofluorescence staining of MCF-7 cell with CSB-RA595981A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Overlay Peak curve showing jurkat cells stained with CSB-RA595981A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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其他：
產(chǎn)品詳情
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Uniprot No.：
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基因名：RPL13
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別名：60S ribosomal protein L13 antibody; BBC1 antibody; Breast basic conserved gene 1 antibody; Breast basic conserved protein 1 antibody; D16S444E antibody; D16S44E antibody; FLJ27453 antibody; FLJ27454 antibody; L13 antibody; MGC117342 antibody; MGC71373 antibody; OK/SW cl.46 antibody; OK/SWcl.46 antibody; Ribosomal protein L13 antibody; RL13_HUMAN antibody; rpl13 antibody
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反應(yīng)種屬：Human, Rat
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免疫原：A synthesized peptide from human RPL13 protein
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：7C2
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IHC, IF, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IHC 1:50-1:200 IF 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules (Probable). The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain (Probable). The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel (Probable). As part of the LSU, it is probably required for its formation and the maturation of rRNAs. Plays a role in bone development.
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基因功能參考文獻(xiàn)：
- Ribosomal protein L13 plays an essential role in the progression of some gastrointestinal malignancies. PMID: 16786168
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亞細(xì)胞定位：Cytoplasm.
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蛋白家族：Eukaryotic ribosomal protein eL13 family
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組織特異性：Higher levels of expression in benign breast lesions than in carcinomas.
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數(shù)據(jù)庫鏈接：
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