PREPL Recombinant Monoclonal Antibody

說明書

中文名稱：

PREPL Recombinant Monoclonal Antibody
貨號：

CSB-RA161851A0HU
規格：

￥1320
圖片：
Flow cytometric analysis of prolyl endopeptidase like expression in H9c2 cells using prolyl endopeptidase like antibody. Green, isotype control; red, prolyl endopeptidase like.

Immunocytochemical staining of HeLa cells with Prolyl endopeptidase like antibody. Nuclei were stained blue with DAPI; Prolyl endopeptidase like was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Immunohistochemistry was performed on paraffin-embedded mouse brain using prolyl endopeptidase like antibody. Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Western blotting analysis using prolyl endopeptidase like antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with prolyl endopeptidase like antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

Q4J6C6
基因名：

PREPL
別名：

Prolyl Endopeptidase Like; Prolyl Endopeptidase-Like; KIAA0436; Putative Prolyl Oligopeptidase; Prolylendopeptidase-Like; EC 3.4.21.83; EC 3.4.21.-; EC 3.4.21; CMS22
反應種屬：

Human, Mouse, Rat
免疫原：

Recombinant Human PREPL protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

15E11
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC, ICC, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000
IHC	1:100-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Serine peptidase whose precise substrate specificity remains unclear. Does not cleave peptides after a arginine or lysine residue. Regulates trans-Golgi network morphology and sorting by regulating the membrane binding of the AP-1 complex. May play a role in the regulation of synaptic vesicle exocytosis.
基因功能參考文獻：
1. we report the first homozygous PREPL point mutation in a girl with typical PREPL deficiency. This syndrome should be considered in the differential diagnosis of hypotonic neonates exhibiting myasthenic symptoms, hyperphagia, and various degrees of ID. PMID: 29483676
2. Deletion of PREPL is associated with Hypotonia-cystinuria syndrome. PMID: 23794250
3. Two novel deletions encompassing the SLC3A1 and PREPL genes have been identified in unrelated hypotonia-cystinuria syndrome patients. PMID: 22796000
4. description of L-leucine transport into bladder carcinoma cells PMID: 12225859
5. A 638-residue variant of PREPL, PREPL A, was identifiied, expressed in Escherichia coli and purified; its secondary structure was similar to that of oligopeptidase B. PMID: 16143824
6. Deletion of PREPL, a gene encoding a putative serine oligopeptidase, in patients with hypotonia-cystinuria syndrome. PMID: 16385448
7. Deletion of the gene results in hypotonia at birth, failure to thrive and growth hormone deficiency PMID: 16913837
8. a deletion of PREPL causes atypical hypotonia-cystinuria syndrome PMID: 18234729
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相關疾病：

Hypotonia-cystinuria syndrome (HCS); Myasthenic syndrome, congenital, 22 (CMS22)
亞細胞定位：

Cytoplasm, cytosol. Golgi apparatus, trans-Golgi network. Cytoplasm, cytoskeleton. Golgi apparatus. Nucleus.
蛋白家族：

Peptidase S9A family
組織特異性：

Expressed in pyramidal neurons of the temporal cortex and neocortex (at protein level). Widely expressed. Expressed at higher level in brain, skeletal muscle, heart and kidney. Expressed at the endplates in the neuromuscular junction.
數據庫鏈接：

HGNC: 30228

OMIM: 606407

KEGG: hsa:9581

STRING: 9606.ENSP00000260648

UniGene: Hs.444349

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