NXF1 Recombinant Monoclonal Antibody

說明書

中文名稱：

NXF1 Recombinant Monoclonal Antibody
貨號：

CSB-RA160494A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: THP-1 whole cell lysate(30μg), Hela whole cell lysate(30μg), HEK293T whole cell lysate(30μg), K562 whole cell lysate(30μg)
All lanes: NXF1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time：2min

IHC image of CSB-RA160494A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA160494A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of Hela cell with CSB-RA160494A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing jurkat cells stained with CSB-RA160494A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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靶點詳情

產品詳情

Uniprot No.：

Q9UBU9
基因名：

NXF1
別名：

Nuclear RNA export factor 1 (Tip-associated protein) (Tip-associating protein) (mRNA export factor TAP), NXF1, TAP
反應種屬：

Human
免疫原：

A synthesized peptide from human NXF1 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

23F2
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex. ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export. Also involved in nuclear export of m6A-containing mRNAs: interaction between SRSF3 and YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export.
基因功能參考文獻：
1. Gain or loss-of-function experiments revealed NXF1 selectively regulates TLR7-driven IRF5 transcriptional activity, suggesting a new role for NXF1 in the IRF5 signaling pathway. PMID: 28578407
2. repeat RNA-sequestration of SRSF1 triggers the NXF1-dependent nuclear export of C9ORF72 transcripts retaining expanded hexanucleotide repeats PMID: 28677678
3. Hepatitis B virus core protein associates with the cellular NXF1-p15 complex via the nuclear export signal motif of hepatitis B virus core protein.Nuclear export of human hepatitis B virus core protein and pregenomic RNA depends on the NXF1-p15 machinery. PMID: 25360769
4. Complementary structural, biochemical and cellular techniques indicated that the formation of a symmetric RNA binding platform generated by dimerization of NXF1:NXT1 facilitates the recognition of CTE-RNA and promotes its nuclear export PMID: 25628361
5. Gag polyprotein synthesis was decreased by NXF1 knockdown. PMID: 25212909
6. TREX is the major complex used to recruit Nxf1 to mRNA in human cells. PMID: 22893130
7. the organization of the NXF1 proline-tyrosine nuclear localization signal reveal unexpected redundancy in the nuclear import pathways used by NXF1. PMID: 21965294
8. The U2AF65 protein served as an adaptor to link expanded CAG RNA to NXF1 for RNA export. PMID: 21725067
9. The crystal structure of the RNA recognition and leucine-rich repeat motifs of TAP bound to one symmetrical half of the CTE RNA is solved. PMID: 21822283
10. these data revealed a redox-regulated chaperone function of PDI in delivering antigenic peptides from TAP to MHC-I. PMID: 21299467
11. Tpr plays an important role in quality control of mRNA trafficked on the Nxf1 pathway. PMID: 21613532
12. These results showed that the interaction between herpes simplex virus type 1 ICP27 and human TAP/NXF1 occurred in living cells upon head-to-tail intramolecular association of ICP27. PMID: 21060739
13. Varicella-zoster virus IE4 protein interacts with SR proteins and exports mRNAs through the TAP/NXF1 pathway PMID: 19924249
14. Depletion of NXF1 or 5,6-dichloro-1-beta-d-ribofuranosyl-benzimidazole treatment had similar effects, inhibiting the nuclear export of several of the H5N1 influenza virus mRNAs. PMID: 20071484
15. The structural and mutational data define a novel RNA-binding site on the Tap protein PMID: 11854490
16. Structure of the C-terminal FG-nucleoporin binding domain of Tap/NXF1 PMID: 11875519
17. Karyopherin beta2B is a nuclear export factor and mediates mRNA export in cooperation with TAP PMID: 12384575
18. structural basis for interaction of UBA domain and FG nucleoporins PMID: 12581645
19. nuclear export sequences regulate human TAP function in mRNA export by direct nuclear pore interactions PMID: 14963046
20. hypophosphorylation of ASF in mRNPs coincides with its higher affinity for TAP PMID: 15184380
21. SR proteins 9G8 and ASF/SF2 exhibit higher affinity for TAP/NXF1 when hypophosphorylated PMID: 15210956
22. concluded that herpes simplex virus type 1 ICP27 and the viral RNAs to which it binds are exported via the TAP/NXF1 export receptor. PMID: 15767397
23. results indicate that Tap regulates expression of its own intron-containing RNA through a CTE (constitutive transport element)-mediated mechanism PMID: 16971948
24. RNA transport element RTE evolved as a high affinity RBM15 ligand to provide a splicing-independent link to NXF1, thereby ensuring efficient nuclear export and expression of retrotransposon transcripts PMID: 17001072
25. evidence presented that the export factor TAP/NXF1 binds poorly to XIST RNA in comparison to exported mRNAs, suggesting that reduced TAP/NFX1 binding may contribute to nuclear retention of XIST RNA PMID: 17333237
26. ORF57 interacts with the cellular export factor REF and with RNA, suggesting that it may provide target mRNAs with access to REF, which mediates nuclear RNA export by binding to TAP/NXF1. PMID: 17609285
27. Homotypic Tap complex can interact with both Nxt1 and nucleoporins in vitro. PMID: 17978099
28. The efficiency and stability of the approach are demonstrated by reconstructing the structure of a two domain region of the 31 kDa nuclear export factor TAP (TIP-associated protein). PMID: 18670889
29. ICP27 is the major export adaptor for HSV-1 mRNA and that it links bound transcripts to the TAP/NXF1 export receptor PMID: 19019956
30. The RNA-binding motif protein 15B (RBM15B/OTT3) acts as cofactor of the nuclear export receptor NXF1. PMID: 19586903
31. oligomerization of Tap affects its interactions with nucleoporins PMID: 17978099
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亞細胞定位：

Nucleus. Nucleus, nucleoplasm. Nucleus speckle. Nucleus, nuclear pore complex. Nucleus envelope. Cytoplasm. Cytoplasm, Stress granule.
蛋白家族：

NXF family
組織特異性：

Expressed ubiquitously.
數據庫鏈接：

HGNC: 8071

OMIM: 602647

KEGG: hsa:10482

STRING: 9606.ENSP00000294172

UniGene: Hs.523739

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