NTHL1 Recombinant Monoclonal Antibody

說明書

中文名稱：

NTHL1 Recombinant Monoclonal Antibody
貨號：

CSB-RA016721A0HU
規格：

￥1320
圖片：
Flow cytometric analysis of Nth like DNA glycosylase 1 expression in HepG2 cells using Nth like DNA glycosylase 1c antibody. Green, isotype control; red, Nth like DNA glycosylase 1.

Immunocytochemical staining of HepG2 cells with Nth like DNA glycosylase 1antibody. Nuclei were stained blue with DAPI; Nth like DNA glycosylase 1 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using Nth like DNA glycosylase 1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Nth like DNA glycosylase 1 antibody and HRP-conjugated goat rabbit secondary antibody respectively.
其他：

產品詳情
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靶點詳情

產品詳情

Uniprot No.：

P78549
基因名：

NTHL1
別名：

NTHL1; Nth Like DNA Glycosylase 1; OCTS3; NTH1; Bifunctional DNA N-Glycosylase/DNA-(Apurinic Or Apyrimidinic Site) Lyase; Endonuclease III-Like Protein 1; DNA Glycosylase/AP Lyase; HNTH1; Bifunctional DNA N-Glycoslyase/DNA-(Apurinic Or Apyrimidinic Site) Lyase; Nth Endonuclease III-Like 1 (E. Coli); Nth (E.Coli Endonuclease III)-Like 1; Nth Endonuclease III-Like 1; DNA Glycoslyase/AP Lyase; EC 4.2.99.18; EC 3.2.2.-; FAP3
反應種屬：

Human, Rat
免疫原：

Recombinant Human NTHL1 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

6G8
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC, ICC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines. Has also 8-oxo-7,8-dihydroguanine (8-oxoG) DNA glycosylase activity. Acts preferentially on DNA damage opposite guanine residues in DNA. Is able to process lesions in nucleosomes without requiring or inducing nucleosome disruption.
基因功能參考文獻：
1. The processing of the lesions was evaluated by purified enzyme cocktails of hNTH1 and hOGG1 as well as with a HeLa cell extract. Interestingly, the yield of double-strand breaks (DSBs) resulting from the processing of the bistranded lesions are appreciably lower when the DNA is treated with the HeLa cell extract compared with the relevant purified enzyme cocktail in both models PMID: 30207305
2. NTHL1 in these extracts was able to excise thymine glycol from both naked DNA and sites in nucleosomes that earlier studies had shown to be sterically accessible. However, the same extracts were able to excise lesions from sterically-occluded sites in nucleosomes only after the addition of Mg2+/ATP. PMID: 28709015
3. WT NTHL1 (human) and Nth (E. coli) are remarkably alike with respect to specificity of glycosylase reaction, and although NTHL1 is a much slower enzyme than Nth, the tighter binding of NTHL1 compensates, resulting in similar kcat/Kd values for both enzymes with each of the substrates tested. For NTHL1 Gln287Ala, specificity for substrates positioned opposite G is lost, but not that of substrates positioned opposite A. PMID: 28292631
4. We therefore found published evidence to support the association between variants in NTHL1 and RPS20 with CRC. PMID: 27713038
5. Both Ntg1 and its human homologue, NTHL1, can be SUMO-modified in response to oxidative stress. PMID: 27839712
6. NTH1 polymorphisms may be associated with non-small cell lung cancer pathogenesis. PMID: 26400813
7. This study extends the description of biallelic mutations in NTHL1 beyond the single c.268C-->T,p.Q90* mutation that was observed previously. PMID: 26559593
8. A homozygous loss-of-function germline mutation in the NTHL1 gene predisposes to a new subtype of BER-associated adenomatous polyposis and colorectal cancer. PMID: 25938944
9. we demonstrated that hNEIL1 and hNTH1 cleave Oz sites as efficiently as 5-hydroxyuracil sites. Thus, hNEIL1 and hNTH1 can repair Oz lesions PMID: 22465744
10. NTH1 is involved in removal of 8-oxoguanine from 8-oxoguanine/guanine mispairs in DNA PMID: 11328882
11. recombinant hNTH1 lacking 55, 72 or 80 residues from the N terminus had four- to fivefold higher activities than the full-length enzyme PMID: 12144783
12. substrate selectivity of mammalian NTH1 and the concomitant selective stimulation of activity by APE1 are indicative of selective repair of oxidative damage in different regions of the genome PMID: 12519758
13. dimerization of hNTH1 involving the N-terminal tail masks the inhibitory effect of this tail and plays a critical role in its catalytic turnover in the cell PMID: 14522981
14. hNTH1 and hNEIL1 but not hNEIL2 excised the two stereoisomers of thymine glycol (5R-Tg and 5S-Tg), but their isomer specificity was markedly different PMID: 14734554
15. search for the factors interacting with NTH1 shows GST-NTH1 fusion protein precipitates proliferating cell nuclear antigen (PCNA) and p53 as well as XPG from human cell-free extracts PMID: 15358233
16. NTH1 is a DNA glycosylase that excise 5-formyluracil, 5-hydroxymethyluracil and Thymine glycol in human cells. PMID: 15533839
17. hNTH1 plays two roles in the processing of radiation damages: repair of potentially lethal single lesions and generation of lethal double strand breaks at clustered damage sites. PMID: 16111924
18. Nth1 released 5R,6S 2'-deoxyribonucleoside diastereoisomer (Tg2) much more rapidly than cis 5S,6R-deoxyribonucleoside diastereoisomer (Tg1) regardless of the opposing purine. Neil1 released Thymine glycol non-stereoselectively. PMID: 16446124
19. The damage specificity of human homologues of Endo III (hNTHl) and Endo VIII (hNEIL1 and hNEIL2) is compared to elucidate the repair role in cells. PMID: 17150535
20. These observations suggest that access to sterically occluded lesions entails the partial, reversible unwrapping of DNA from the histone octamer, allowing hNTH1 to capture its DNA substrate when it is in an unwound state. PMID: 17923696
21. Downregulation of NTH1 is associated with gastric cancer. PMID: 19414504
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相關疾病：

Familial adenomatous polyposis 3 (FAP3)
亞細胞定位：

Nucleus. Mitochondrion.
蛋白家族：

Nth/MutY family
組織特異性：

Widely expressed with highest levels in heart and lowest levels in lung and liver.
數據庫鏈接：

HGNC: 8028

OMIM: 602656

KEGG: hsa:4913

STRING: 9606.ENSP00000219066

UniGene: Hs.66196

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