NDUFA13 Recombinant Monoclonal Antibody

說明書

中文名稱：

NDUFA13 Recombinant Monoclonal Antibody
貨號：

CSB-RA104843A0HU
規格：

￥1320
圖片：
Western Blot
, Positive WB detected in: Hela whole cell lysate(30μg), HepG2 whole cell lysate(30μg), HEK293 whole cell lysate(30μg), MCF7 whole cell lysate(30μg), U251 whole cell lysate(30μg), THP-1 whole cell lysate(30μg)
All lanes: NDUFA13 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time：120s

IHC image of CSB-RA104843A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA104843A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Overlay Peak curve showing jurkat cells stained with CSB-RA104843A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

Q9P0J0
基因名：

NDUFA13
別名：

2700054G14Rik antibody; AU022060 antibody; B16.6 antibody; CDA016 antibody; Cell death regulatory protein antibody; Cell death regulatory protein GRIM-19 antibody; CGI-39 antibody; CGI39 protein antibody; CI-B16.6 antibody; Complex I-B16.6 antibody; FLJ58045 antibody; FLJ59191 antibody; Gene associated with retinoic and IFN-induced mortality 19 protein antibody; Gene associated with retinoic and interferon-induced mortality 19 protein antibody; Gene associated with retinoic interferon induced mortality 19 protein antibody; GRIM-19 antibody; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13 antibody; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 antibody; NADH ubiquinone oxidoreductase B16.6 subunit antibody; NADH-ubiquinone oxidoreductase B16.6 subunit antibody; NDUAD_HUMAN antibody; NDUFA13 antibody; RGD1565358 antibody
反應種屬：

Human
免疫原：

A synthesized peptide from human NDUFA13 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

4B1
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Prevents the transactivation of STAT3 target genes. May play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes.
基因功能參考文獻：
1. These results indicated that rAd-Grim-19 may regulate tumor cell growth and apoptosis. Additionally, the results demonstrated that rAdGrim19 led to beneficial outcomes and prolonged the survival of esophageal tumorbearing mice. In conclusion, the present study demonstrated that rAdGrim19 may have potential as an antitumor agent for esophageal neoplasms PMID: 29488605
2. Low GRIM19 expression is associated with radiation resistance in osteosarcoma. PMID: 30005830
3. GRIM-19 is a potential predictor of prognosis and disease recurrence in high grade serous carcinoma PMID: 29254797
4. Promoting the expression of GRIM19 may yield therapeutic benefits in the treatment of RA. PMID: 29306209
5. MiR-6743-5p may act as an oncomiRNA in glioma by targetting GRIM-19 and STAT3. PMID: 29074558
6. GRIM-19 suppresses the proliferation and invasion of cutaneous squamous cell carcinoma cells associated with downregulation of STAT3 signaling. PMID: 28926927
7. Mitochondrial GRIM-19 could not only serve as an valuable prognostic biomarker for gastric cancer development, but also as a potential therapeutic target for STAT3-dependent carcinogenesis of Gastric cancer. PMID: 27167343
8. GRIM-19 expression in cervical cancer cells could inhibit telomerase activity by inhibiting the transactivation of the hTERT promoter by E6, thereby promoting cervical cancer cell senescence. PMID: 27142689
9. NDUFA13 deficiency may be associated with asthenozoospermia through the disturbance of spermatozoa mitochondrial membrane potential and by increasing apoptosis and intracellular reactive oxygen species PMID: 27789183
10. GRIM-19 overexpression suppressed hepatocellular carcinoma (HCC) growth and downregulated AKT1 expression, suggesting that GRIM-19 might play a crucial role in hepatocarcinogenesis through negatively regulating the PI3K/AKT signaling pathway. PMID: 25550785
11. Low GRIM-19 expression is associated with paclitaxel resistance in cervical cancer. PMID: 26810068
12. activation of AMPKalpha by metformin was associated with a reversal of the suppressed GRIM-19 expression in H9C2 cells, the fold of changes in GRIM-19 expression by metformin were much less in HeLa cells PMID: 27101310
13. Data suggest that tumor expression of Ki67 (antigen Ki-67) and GRIM19 correlate with malignancy in thyroid Hurthle cell (HC) tumors; variable expression of Ki67 and GRIM19 may helped differentiate HC carcinoma from HC adenoma. PMID: 26188382
14. Transfection with eukaryotic plasmid for the simultaneous expression of GRIM19 and LKB1 more effectively suppressed the growth of breast cancer in vitro and in vivo, and may therefore have therapeutic potential for the treatment of human breast cancer. PMID: 26458553
15. Aberrant endometrial expression of GRIM-19 was associated with adenomyosis through the regulation of apoptosis and angiogenesis. PMID: 26769301
16. We established here a correlation between the first mutation identified in the NDUFA13 gene, which induces Mitochondrial complex I instability and a severe but slowly evolving clinical presentation affecting the central nervous system. PMID: 25901006
17. Upregulation of GRIM-19 also suppressed the secretion of urokinase-type plasminogen activator (u-PA), matrix metalloproteinase (MMP)-2, MMP-9 and vascular endothelial growth factor (VEGF). PMID: 25955394
18. GRIM-19 expression is closely associated with colorectal cancer progression and might be a very promising prognostic biomarker PMID: 26363526
19. Upregulation of GRIM-19 in oral squamous cell carcinoma cells significantly inhibited cell proliferation, migration and invasion in vitro and suppressed tumor growth in vivo. PMID: 25174621
20. decreased GRIM-19 expression due to promoter hypermethylation may be important in head and neck carcinogenesis by promoting cell proliferation and regulating metabolic activity PMID: 25575809
21. Simultaneous expression of ADAM10-specific siRNA and GRIM19 in HepG2 cancer cells significantly inhibited the proliferation, migration and invasion. PMID: 25242535
22. The GRIM-19 deficiency in the villus may be associated with missed abortion via increasing apoptosis and affecting angiogenesis. PMID: 25455534
23. GRIM-19 may regulate the differentiation of normal cervical tissue, and a decrease in GRIM-19 may be the result of HR-HPV infection, which in turn leads to the malignant transformation of the cells. PMID: 24690422
24. GRIM-19 mutations are associated with oral squamous cell carcinoma. PMID: 23851499
25. role in macrophage apoptosis induced by H5N1 virus PMID: 23529854
26. silencing of survivin and over-expression of GRIM-19 can significantly inhibit the growth and induce the apoptosis of Hep-2 laryngeal cancer cells in vitro and in vivo. PMID: 24133585
27. Downregulation of GRIM-19 promotes HIF1alpha synthesis. PMID: 23580587
28. Expressions of GRIM-19, NDUFS3, and extracellular matrix elements are correlated with invasive capabilities of breast cancer cell lines. PMID: 23630608
29. Down-regulation of GRIM-19 is associated with STAT3 overexpression in breast carcinomas. PMID: 23618357
30. GRIM-19 expression is closely correlated with histological grading and p-STAT3 in hepatocellular carcinoma PMID: 22492280
31. Our study found that GRIM-19 expression in lung cancer exhibits a relationship with the histological type and clinical stage PMID: 22573109
32. Tumor-derived mutations in the gene associated with retinoid interferon-induced mortality (GRIM-19) disrupt its anti-signal transducer and activator of transcription 3 (STAT3) activity and promote oncogenesis PMID: 23386605
33. import of the transcription factor STAT3 into mitochondria depends on GRIM-19, a component of the electron transport chain PMID: 23271731
34. GRIM-19 inhibits the STAT3 signaling pathway and sensitizes gastric cancer cells to radiation. PMID: 23124042
35. GRIM-19 plays a critical role not only at the origin but also in the invasive progression of hepatocellular carcinoma. PMID: 22105514
36. GRIM-19 expression was lower in gliomas & negatively correlated with malignancy. Downregulation enhanced cell proliferation & migration, but overexpression did the opposite. GRIM-19 exerts its role through the non-STAT3 signaling pathway. PMID: 21827581
37. GRIM-19 function in cancer development PMID: 21664299
38. The expressions of survivin and GRIM-19 may be closely correlated with the pathogenesis of prostate cancer. PMID: 21351527
39. These data suggest that GRIM-19 can block E6/E6AP complex; and synergistically suppress cervical tumor growth with p53. PMID: 21765936
40. reduced mRNA and protein levels in lung adenocarcinoma tissues PMID: 21040996
41. GRIM-19 expression is down-regulated in non-small cell lung cancer. PMID: 19622307
42. Results identify a major role for the N terminus of GRIM-19 in mediating its tumor-suppressive actions. PMID: 20595633
43. Alternatively spliced GRIM-19 mRNA is associated with kidney cancer. PMID: 20505682
44. GRIM-19/CDKN2A synergistically suppressed cell cycle progression via inhibiting E2F1-driven gene expression. PMID: 20522552
45. Loss of GRIM-19 is associated with invasion and metastasis of human gastric cancer. PMID: 20478305
46. Data show that restoration of GRIM-19 levels reestablishes the control over STAT3-dependent gene expression and tumor growth in vivo. PMID: 19642906
47. results collectively indicate that viral interferon regulatory factor 1 modulates interferon/retinoic acid-cell death signals via interactions with GRIM19 PMID: 12163600
48. GRIM-19 is an inhibitor of signal transducer and activator of transcription 3 PMID: 12867595
49. GRIM-19 may be a key component in NOD2-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes PMID: 15753091
50. The IFN-beta- and tretinoin-induced GRIM-19 is upregulated during focal cerebral ischemia. PMID: 17523870
顯示更多

收起更多
相關疾?。?/div>
Hurthle cell thyroid carcinoma (HCTC)
亞細胞定位：

Mitochondrion inner membrane; Single-pass membrane protein; Matrix side. Nucleus.
蛋白家族：

Complex I NDUFA13 subunit family
組織特異性：

Widely expressed, with highest expression in heart, skeletal muscle, liver, kidney and placenta. In intestinal mucosa, down-regulated in areas involved in Crohn disease and ulcerative colitis.
數據庫鏈接：

HGNC: 17194

OMIM: 607464

KEGG: hsa:51079

STRING: 9606.ENSP00000423673

UniGene: Hs.534453

Most popular with customers

YWHAG Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
YWHAB Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF, FC

Species Reactivity: Human, Mouse, Rat
XRCC5 Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF

Species Reactivity: Human
XPNPEP1 Recombinant Monoclonal Antibody

Applications: ELISA, IHC, FC

Species Reactivity: Human
XDH Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
WT1 Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
WDR5 Recombinant Monoclonal Antibody

Applications: ELISA, IHC, FC

Species Reactivity: Human
VIM Recombinant Monoclonal Antibody

Applications: ELISA, IHC, IF

Species Reactivity: Human