NCF2 Recombinant Monoclonal Antibody

說明書

中文名稱：

NCF2 Recombinant Monoclonal Antibody
貨號：

CSB-RA991818A0HU
規格：

￥1320
圖片：
IHC image of CSB-RA991818A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA991818A0HU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Overlay Peak curve showing jurkat cells stained with CSB-RA991818A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
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靶點詳情

產品詳情

Uniprot No.：

P19878
基因名：

NCF2
別名：

Neutrophil cytosol factor 2 (NCF-2) (67 kDa neutrophil oxidase factor) (NADPH oxidase activator 2) (Neutrophil NADPH oxidase factor 2) (p67-phox), NCF2, NOXA2 P67PHOX
反應種屬：

Human
免疫原：

A synthesized peptide from human NCF2 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

9B8
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, FC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
基因功能參考文獻：
1. We analyzed the clinical and laboratory findings of CGD with mutations in the NCF2 gene from amongst our cohort of CGD patients. A homozygous mutation (c.835_836delAC, p.T279fsX294), a deletion in NCF2 gene was found in two cases. In the third case, two heterozygous mutations were detected, IVS13-2A>T on one allele and c.1099C>T (p.) on the other allele. PMID: 28035544
2. All investigated patients presented the same mutation (c.257 + 2T > C) in NCF2 gene. We show that this mutation is responsible for a drastic decrease of p67phox mRNA and leads to the skipping of exon 3 detected in the low amount of residual mRNA. PMID: 27220316
3. Phosphoinositol 3-phosphate regulates reactive oxygen species production by maintaining p40phox and p67phox at the phagosomal membrane. PMID: 28096301
4. TLR4- and TLR2-induced IRAK-ERK pathway cross-talks with p67phox-Nox-2 for reactive oxygen species generation, thus regulating IL-1beta transcription and processing in monocytic cells. PMID: 26320741
5. Skeletal muscle protein expression of the NADPH oxidase subunits p22(phox), p47(phox), and p67(phox) was increased in obese relative to lean subjects, where p22(phox) and p67(phox) expression was attenuated by exercise training in obese subjects. PMID: 27765769
6. A novel homozygous mutation in NCF2. PMID: 26272171
7. results reveal an essential role for the Cys-Gly-Cys triad in Nox2 in binding p67(phox), seconded by an additional binding region, comprising residues C terminal to Cys-Gly-Cys. The 2 regions interact with distinct partner sites in p67(phox). PMID: 26160850
8. This model assigns a central role to Arg-395 in the structure and stability of the quaternary NCF2/NCF4/VAV1/RAC1 NADPH oxidase complex. PMID: 25795782
9. Data indicate that arachidonic acid induces the direct interaction of Rac-GTP-bound p67(phox) with the C-terminal cytosolic region of phagocyte NADPH oxidase Nox2. PMID: 25056956
10. Four novel mutations in the NCF1, NCF2, and CYBB genees have been identified in chronic granulomatous disease patients in Morocco. PMID: 24596025
11. Results not only establish allelic heterogeneity within NCF2 associated with SLE, but also emphasize the utility of multi-ethnic cohorts to identify predisposing variants explaining additional phenotypic variance of complex diseases like SLE. PMID: 24163247
12. NCF2 in Asian populations shows a pattern of diversity characterized by a differentiated haplotype structure. PMID: 23821607
13. Results provide insight into the redox-sensitive signaling mechanism that mediates cell survival involving p53 and its novel target NCF2/p67phox. PMID: 23187810
14. Case Report: report defects in NCF-2, teh gene encoding p67-phox, in four cases of chronic granulomatous disease. PMID: 23264412
15. Eight novel mutations in CYBB and NCF2 genes were identified in patients with chronic granulomatous disease. PMID: 22562447
16. This variant reduced binding of the NCF2 gene product p67(phox) to RAC2. This study found a novel genetic association of RAC2 with Crohn's disease (CD) and replicated the previously reported association of NCF4 with ileal CD. PMID: 21900546
17. NCF2 is strongly associated with increased risk of childhood- and adult-onset systemic lupus erythematosus through a single nonsynonymous coding mutation (H389Q) in exon 12. PMID: 22203994
18. p67(phox) has a critical role to support for reactive oxygen species production on the level of individual phagosomes. PMID: 21954286
19. Association analysis identified five SLE susceptibility genes reaching genome-wide levels of significance : NCF2 ,IKZF1 ,IRF8 ,IFIH1 , and TYK2 PMID: 22046141
20. The genetic variation in the NCF2 gene was found to associate with SLE in US and European populations PMID: 20842512
21. High NCF2 expression in the cytoplasm is associated with uterine cervix carcinogenesis. PMID: 21119665
22. the extended activation domain of p67(phox) (amino acids 190-210) containing the D(Y/F)LGK motif plays an essential role in oxidase activation probably by interacting with gp91(phox). PMID: 20679349
23. mutations in CYBB, NCF1, CYBA or NCF2 may play a role in chronic granulomatous disease PMID: 18546332
24. There is an increased expression of NADPH oxidase p47(-PHOX) and p67(-PHOX) factor in idiopathic pulmonary fibrosis patients. PMID: 17651608
25. All mutations and some polymorphisms identified in the NCF2 gene in the autosomal forms of chronic granulomatous disease are listed. Review. PMID: 20167518
26. Here we show that p67(phox)adopts an elongated conformation when it exists not only as a monomer but also as the heterotrimer PMID: 20375610
27. These findings identify the activation of PKC delta and NADPH oxidase as crucial steps in retinoic acid-induced neuroblastoma cell differentiation. PMID: 20074641
28. Alu-induced deletion of the TPR4 domain of p67-phox leads to loss of function and accelerated degradation of the protein. PMID: 19953534
29. In a cell-free system, covalent binding between C-terminal-truncated p67phox and rac in the correct fusion order produces a more stable complex than the individual components and significantly influences the duration of fusion-produced oxidase activation. PMID: 11705402
30. detailed study of the protein-protein interactions that occur in the p40-p47-p67(phox) complex of the resting oxidase PMID: 11796733
31. p22(phox), gp91(phox), p47(phox), p67(phox), and p40(phox) existed as a functional complex in the cytoskeletal fraction. PMID: 11893732
32. Val204 in p67(phox), previously shown to be required for NADPH oxidase activity under cell-free conditions, was found to be essential for superoxide production by intact COS-phox cells. PMID: 11929750
33. effect of cPLA2 on its translocation PMID: 12101222
34. NAD(P)H oxidase subunits p47(phox) and p67(phox) are expressed in platelets; and NAD(P)H oxidase-dependent platelet superoxide anion release increases platelet recruitment. PMID: 12130503
35. p67phox and p47phox have roles in regulating a change of conformation in cytochrome b558, which initiates the electron transfer in NADPH oxidase activation PMID: 12719414
36. NOXO1, p47phox, and p67phox regulate Nox3 PMID: 15181005
37. NAD(P)H oxidase activity is associated with increased protein levels of p22phox, p47phox, and p67phox, and increased p22phox and nox2 (gp91phox) mRNA expression. PMID: 15256399
38. Increased expression and activity of NAD(P)H oxidase subunits and xanthine oxidase, in part mediated through angiotensin II and PKC-dependent pathways, are important mechanisms underlying increased oxidative stress in human coronary artery disease PMID: 16293794
39. Here we show that the p47(phox)-p67(phox) interaction is disrupted not only by deletion of the PRR but also by substitution for basic residues in the extra-PRR (K383E/K385E). PMID: 16297854
40. Expression of p67(phox) is regulated through mechanisms that include modulation of transcription and translation. PMID: 16310324
41. These results indicate that Hcy (homocysteine)-stimulated superoxide anion production in monocytes is regulated through PKC-dependent phosphorylation of p47phox and p67phox subunits of NADPH oxidase. PMID: 16626305
42. NADPH oxidase assembly from p67phox was studies at the single-cell level. PMID: 16987007
43. chemoattractant-stimulated superoxide production can be amplified by a positive feedback loop in which p67(phox) targets Vav1-mediated Rac activation PMID: 17060455
44. These data clearly identify PLAGL2 as a novel regulator of NCF2/p67phox gene expression as well as NADPH oxidase activity and contribute to a greater understanding of the transcriptional regulation of NCF2. PMID: 17462995
45. a novel single nucleotide polymorphism in the promoter region PMID: 17712795
46. Single nucleotide polymorphism leads to alternative splicing without altering gene expression or respiratory burst activity. PMID: 17910042
47. p40(phox) translocates p67(phox) to the region of the cytochrome and subsequently switches the oxidase to an activated state dependent upon PtdIns(3)P and SH3 domain engagement. PMID: 18029359
48. As(2)O(3) induced phosphorylation and membrane translocation of the NADPH oxidase subunit p47(phox) and it also increased translocation of Rac1 and p67(phox). PMID: 18424721
49. autosomal recessive CGD due to NCF-2 gene mutations, and a novel homozygous and hypomorphic NCF-2 gene mutation was found. PMID: 18625437
50. p67(phox)-SH3(N) specifically functions in gp91(phox)/Nox2 activation probably via facilitating oxidase assembly. PMID: 19116138
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相關疾病：

Granulomatous disease, chronic, cytochrome-b-positive 2, autosomal recessive (CGD2)
亞細胞定位：

Cytoplasm.
蛋白家族：

NCF2/NOXA1 family
數據庫鏈接：

HGNC: 7661

OMIM: 233710

KEGG: hsa:4688

STRING: 9606.ENSP00000356505

UniGene: Hs.587558

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