MCM3 Recombinant Monoclonal Antibody
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中文名稱：MCM3 Recombinant Monoclonal Antibody
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貨號：CSB-RA145595A0HU
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規格：￥1320
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圖片：
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Flow cytometric analysis of MCM3 expression in HepG2 cells using MCM3 antibody. Green, isotype control; red, MCM3. -
Immunocytochemical staining of HepG2 cells with MCM3 antibody. Nuclei were stained blue with DAPI; MCM3 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. -
Western blotting analysis using MCM3 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MCM3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
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其他：
產品詳情
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Uniprot No.：
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基因名：
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別名：MCM3; Minichromosome Maintenance Complex Component 3; DNA Polymerase Alpha Holoenzyme-Associated Protein P1; DNA Replication Licensing Factor MCM3; RLF Subunit Beta; P1-MCM3; P102; MCM3 Minichromosome Maintenance Deficient 3 (S. Cerevisiae); Minichromosome Maintenance Deficient (S. Cerevisiae) 3; MCM3 Minichromosome Maintenance Deficient 3; Replication Licensing Factor, Beta Subunit; Minichromosome Maintenance Deficient 3; Cervical Cancer Proto-Oncogene 5; DNA Replication Factor MCM3; HRlf Beta Subunit; EC 3.6.4.12; HCC5; P1.H; RLFB
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反應種屬：Human, Mouse, Rat
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免疫原：Recombinant Human MCM3 protein
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：5A9
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, FC, ICC
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推薦稀釋比：
Application Recommended Dilution WB 1:1000-1:5000 FC 1:200-1:2000 ICC 1:100-1:1000 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
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基因功能參考文獻：
- this study shows that MCM3 is a novel proliferation marker in oral squamous cell carcinoma PMID: 27258565
- High MCM3 expression is associated with bone metastasis and advanced human prostate cancer. PMID: 28424404
- reveal the Minichromosome Maintenance Complex to be a critical and directly regulated node within the miR-183 signaling network in MYCN-amplified neuroblastoma cells. Randomly selected MCMs 3 and 5 were experimentally confirmed as direct targets of miR-183. PMID: 27239679
- The results of our preliminary study emphasize the need for future research on MCM-3 as a sensitive proliferation marker, providing an alternative to Ki-67, in cases of various major salivary gland epithelial tumors in children and adolescents. PMID: 28547962
- These data establish new functions for KEAP1 within the nucleus and identify MCM3 as a novel substrate of the KEAP1-CUL3-RBX1 E3 ligase. PMID: 27621311
- The aim of this study is to analyze the immunoexpression of Ki67, p53, MCM3 and PCNA markers in epithelial remnants of dental follicles of impacted teeth and to identify a possible correlation between the immunoexpression of these markers in dentigerous cysts and keratocystic odontogenic tumors. PMID: 27516012
- High MCM-3 expression correlated with Cutaneous T-cell Lymphomas. PMID: 26504025
- normal DNA replication and replication checkpoint activation is regulated through the novel phosphorylation of MCM3 by Chk1 PMID: 25809478
- The relatively lower MCM3 protein expression in FVPTC. PMID: 23821456
- Expression of KB cell MCM3 was not affected by doxorubicin. PMID: 24344040
- present study aimed to investigate the relationship between expression of minichromosome maintenance proteins (MCM-3, MCM-7), metallothioneins (MT-I/II, MT-III), and Ki-67 in 103 ovarian cancer cases, mostly of the serous histological type PMID: 24324072
- High MCM3 expression is associated with mucoepidermoid carcinomas and adenoid cystic carcinomas in salivary gland tumors. PMID: 23886132
- Relatively lower MCM3 protein expression in follicular variant of papillary thyroid carcinoma comparing to classical type could be due to a different tumorigenic pathway favored in this type of tissue. PMID: 23821456
- These findings provide validation of the utility of MCM3 expression as an independent biomarker for prognostication of patients with primary melanoma PMID: 22805320
- excess of MCM3 up-regulates the phosphorylation of CHK1 Ser-345 and CDK2 Thr-14. PMID: 21965652
- MCM3 gene expression is decreased in follicular variant of papillary thyroid carcinoma, contrary to expectation. PMID: 21509594
- Using U373MG cells we found that HCMV IE86 protein was bound to Mcm3, but did not inhibit the cellular DNA synthesis. PMID: 20545442
- MCM3 may be a more reliable proliferation marker than Ki-67 in accessing the growth of tumor and evaluating tumor aggressiveness of papillary thyroid carcinoma. PMID: 19818763
- The MCM3 acetylase MCM3AP inhibits initiation, but not elongation, of DNA replication via interaction with this protein PMID: 12226073
- Elevated MCM3 protein expression is associated with neoplasms PMID: 15623617
- MCM3 is overexpressed in human astrocytic tumors and elicits a cancer-restricted humoral immune response in some patients with brain neoplasms PMID: 15671553
- ORC1 and Mcm3 proteins are required for DNA replication in protein extracts from human cells PMID: 16537544
- analysis of carboxyl-terminal MCM3 phosphorylation sites and their motifs PMID: 17244605
- These data reveal that CDK-dependent MCM3 phosphorylation contributes to the regulated formation of the MCM2-7 complex. PMID: 18524952
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亞細胞定位：Nucleus. Chromosome.
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蛋白家族：MCM family
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