MATR3 Recombinant Monoclonal Antibody
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中文名稱：MATR3 Recombinant Monoclonal Antibody
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貨號：CSB-RA221924A0HU
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規(guī)格：￥1320
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圖片：
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Western Blot
Positive WB detected in: K562 whole cell lysate(30μg), NIH/3T3 whole cell lysate(30μg)
All lanes: Matrin 3 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 95 kDa
Observed band size: 125 kDa
Exposure time：1min -
IHC image of CSB-RA221924A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
IHC image of CSB-RA221924A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
Immunofluorescence staining of SH-SY5Y cell with CSB-RA221924A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA221924A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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Uniprot No.：
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基因名：MATR3
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別名：Matrin-3, MATR3, KIAA0723
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反應種屬：Human, Mouse
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免疫原：A synthesized peptide from human MATR3 protein
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：10C3
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, WB, IHC, IF, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：May play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. In association with the SFPQ-NONO heteromer may play a role in nuclear retention of defective RNAs. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway. May bind to specific miRNA hairpins.
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基因功能參考文獻：
- Depletion of SAFB1 reduced FUS's localization to chromatin-bound fraction and splicing activity, suggesting SAFB1 could tether FUS to chromatin compartment thorough N-terminal DNA-binding motif. Moreover, FUS interacts with another nuclear matrix-associated protein, Matrin3. PMID: 27731383
- Using a novel RNA pulldown approach that utilized endogenous S1-tagged PINCR (a p53-regulated long noncoding RNA), the authors show that PINCR associates with the enhancer region of these genes by binding to RNA-binding protein Matrin 3 that, in turn, associates with p53. PMID: 28580901
- Matrin3 physically interacts with intronic pyrimidine-rich sequences and controls alternative splicing. PMID: 28695676
- It may not be a common genetic factor in Chinese amyotrophic lateral sclerosis patients. PMID: 26708275
- Its mutations strengthens the role of RNA metabolism in amyotrophic lateral sclerosis etiology. PMID: 26493020
- A missense mutation in MATR3 was identified in myopathy patients undergoing a needle electromyography. PMID: 26899464
- Our findings indicate that mutations in Matrin 3 that are associated with ALS and myopathy do not dramatically alter the normal localization of the protein or readily induce inclusion formation. PMID: 26528920
- Three-dimensional mapping of the lamin A-matrin-3 interface showed that the LMNA truncating mutation Delta303, which lacks the matrin-3 binding domain, was associated with an increased distance between lamin A and matrin-3. PMID: 25948554
- Suppressing Matrin 3 powers a heightened and broader ZAP restriction of HIV-1 gene expression. PMID: 26129669
- MATR3 mutation is identified to be a possible cause of amyotrophic lateral sclerosis. PMID: 25771394
- No mutations were identified, indicating that MATR3 mutations are not a common cause of Amyotrophic lateral sclerosis in Australian familial cases with predominately European ancestry PMID: 25523636
- MATR3 gene disruption is associated with bicuspid aortic valve, aortic coarctation and patent ductus arteriosus. PMID: 25574029
- Mutations in MATR3 are rare in French familial ALS and ALS with FTLD patients. PMID: 25158920
- This study shows a high risk of abnormal respiratory function with progressive worsening in MATR3 myopathy. PMID: 25677933
- Nuclear matrix protein Matrin3 regulates alternative splicing and forms overlapping regulatory networks with PTB. PMID: 25599992
- the pY RNA1-s2/Matr3 interaction could play a role in vision PMID: 24558381
- Sixteen patients from 6 families with late onset distal myopathy associated with the p.S85C MATR3 mutation were characterized. PMID: 25154462
- This study identified mutations in MATR3 in ALS kindreds. also observed MATR3 pathology in ALS-affected spinal cords with and without MATR3 mutations. PMID: 24686783
- Data suggest that the cellular level of MATR3, known to be highly regulated, modulates the stability of a group of gene transcripts. PMID: 21858232
- Rev needs MATR3 to promote the cytoplasmic accumulation and translation of unspliced RRE-containing mRNA. PMID: 21771348
- MATR3 binds viral RNA and is required for the Rev/RRE mediated nuclear export of unspliced HIV-1 RNAs. PMID: 21771346
- Matrin 3 binds Rev RNA to stabilize HIV-1 transcripts leading to increased cytoplasmic expression. PMID: 21771347
- MATR3 to be a novel ATM target in response to DNA damage. PMID: 20421735
- matrin 3 plays a significant role in controlling cell growth and proliferation PMID: 21182838
- study of the association of matr3 with chromosome territories and identification of potential interacting proteins PMID: 19562669
- Data show that the nuclear matrix protein matrin 3, cytoskeletal motor protein HMP, and the circadian clock protein lark were significantly decreased in fetal Down syndrome brain. PMID: 12469345
- These results suggest that the functions of matrin 3 could be regulated by both Ca(2+)-dependent interaction with calmodulin and caspase-mediated cleavage. PMID: 17658460
- the spatial proximities among a constellation of functionally related sites that are found within euchromatic regions of the cell nucleus including: HP1gamma, RNA polymerase II, matrin 3, and SAF-A sites PMID: 18618731
- nonconservative S85C missense mutation in vocal cord and pharyngeal weakness with distal myopathy PMID: 19344878
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相關疾病：Amyotrophic lateral sclerosis 21 (ALS21)
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亞細胞定位：Nucleus matrix.
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數據庫鏈接：
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