MAS1L Recombinant Monoclonal Antibody
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中文名稱：MAS1L重組抗體
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貨號：CSB-RA971033A0HU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Jurkat whole cell lysate, Rat liver tissue
All lanes: MAS1L antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43 kDa
Observed band size: 36-50 kDa -
Immunofluorescence staining of MCF-7 cell with CSB-RA971033A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 595-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Immunofluorescence staining of Hela cell with CSB-RA971033A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 595-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Overlay Peak curve showing Hela cells stained with CSB-RA971033A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions.
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其他：
產品詳情
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產品描述：CSB-RA971033A0HU是靶向MAS1L蛋白的重組單克隆抗體，適用于多種實驗場景的科研需求。該抗體針對MAS1L受體特異性設計，可精確識別其天然構象表位，在多種驗證實驗中展現出優異的性能：Western Blot（WB）推薦稀釋比例1:500-1:2000，可清晰檢測目標蛋白條帶；免疫熒光（IF）和流式細胞術（FC）分別在1:50-1:200稀釋范圍內呈現高信噪比染色效果，適用于亞細胞定位及表面標志物分析。MAS1L作為G蛋白偶聯受體家族成員，參與調控細胞信號轉導和組織穩態，本產品可支持其在細胞功能研究（如增殖調控、分化機制探索）及疾病模型（如心血管或腫瘤相關基礎研究）中的表達分析。經ELISA驗證，該抗體具備高批次穩定性與低交叉反應性，可滿足復雜樣本的定量檢測需求。適用于體外培養細胞、組織裂解液等樣本類型，為受體功能研究、信號通路解析及分子互作實驗提供可靠工具。
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Uniprot No.：
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基因名：
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別名：Mas-related G-protein coupled receptor MRG (MAS-R) (MAS1-like), MAS1L, MRG
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反應種屬：Human, Rat
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免疫原：A synthesized peptide derived from human MAS1L
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：15B8
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, IF, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IF 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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基因功能參考文獻：
- Mas, MrgD, and MRG mediated Ang IV-stimulated AA release that was highest for Mas. While Ang III activated Mas and MrgX2, Ang II stimulated AA release via Mas and MRG. PMID: 18636314
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亞細胞定位：Cell membrane; Multi-pass membrane protein.
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蛋白家族：G-protein coupled receptor 1 family, Mas subfamily
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數據庫鏈接：
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